对西花蓟马高效金龟子绿僵菌菌株筛选及在花生田间的应用效果

doi:10.16409/j.cnki.2095-039x.2021.03.013

摘要/Abstract

摘要： 西花蓟马Frankliniella occidentalis是一种世界性检疫害虫，对包括花生在内的农作物造成极大威胁，金龟子绿僵菌在西花蓟马生物防治中可发挥重要作用。本文从23株金龟子绿僵菌中筛选出菌株M18-8-4，经紫外诱变得到F1、F2代菌株，测定了其及两代诱变菌株对西花蓟马的室内毒力，并对筛选到的高毒力菌株进行田间药效试验。室内毒力测定结果显示，在相同浓度孢子菌悬液下，诱变菌株F2-M18-8-4对西花蓟马的毒力最强，致死中时为2.13 d。以西花蓟马的校正虫口减退率、花生校正保叶效果和增产效果评价了金龟子绿僵菌F2-M18-8-4和3种化学农药（吡虫啉、噻虫嗪、乙基多杀菌素）对西花蓟马的田间防治效果。施药7 d、10 d后，金龟子绿僵菌F2-M18-8-4与3种化学农药对西花蓟马校正虫口减退率无差异；施药15 d后，金龟子绿僵菌F2-M18-8-4对西花蓟马的校正虫口减退率显著高于这3种化学农药，达79.52%，花生校正保叶效果最高，达63.58%，其次为乙基多杀菌素58.11%。在增产效果上，金龟子绿僵菌F2-M18-8-4防治西花蓟马对花生增产效果最好，达9.09%；其次为乙基多杀菌素7.40%。本研究结果表明筛选高毒力金龟子绿僵菌菌株，在适合的时机防治西花蓟马，可部分替代化学农药，并发挥良好的防治效果。

关键词: 金龟子绿僵菌, 西花蓟马, 毒力, 防治效果, 花生

Abstract: Frankliniella occidentalis is a worldwide quarantine pest, which can cause serious yield reduction of many leguminous host plants, including peanuts. Metarhizium anisopliae plays an important role in biological control of F. occidentalis. In this study, the M18-8-4 Strains were screened out from 23 M. anisopliae strains, of which the F1 and F2 strains were obtained by UV mutagenesis. In this study, the high toxic strains were obtained through toxicity tests in the laboratory, and their control of F. occidentalis was tested in the field. The results showed that, when tested at the same concentration of spore suspension, the strain F2-M18-8-4 had the strongest laboratory toxicity to F. occidentalis, with a median lethal time of 2.13 d. The control efficacies of F2-M18-8-4 and 3 chemical pesticides on F. occidentalis were evaluated in terms of the corrected population decline rate of F. occidentalis, the corrected peanut leaf protection rate, and the peanut yield increase rate. The corrected population decline rate of F. occidentalis did not differ significantly between F2-M18-8-4 and the 3 chemical pesticides at 7 days and 10 days post application, whereas at 15 days post application, the corrected population decline rate of F. occidentalis was higher in F2-M18-8-4 than in the 3 pesticides reaching 79.52%; and the peanut leaf protection rate was the highest, up to 63.58%, followed by Spinetoram at 58.11%. The peanut yield increase rate of F2-M18-8-4 was 9.09%. The results suggest the highly virulent strains can substitute for chemical pesticides in the control of F. occidentalis.

Key words: Metarhizium anisopliae, Frankliniella occidentalis, virulence, control effect, peanut

中图分类号:

裴松松, 吴轩, 李瑞军, 赵丹, 陆秀君, 郭巍. 对西花蓟马高效金龟子绿僵菌菌株筛选及在花生田间的应用效果[J]. 中国生物防治学报, 2021, 37(4): 732-739.

PEI Songsong, WU Xuan, LI Ruijun, ZHAO Dan, LU Xiujun, GUO Wei. Screening of Efficient Metarhizium anisopliae Strains for Frankliniella occidentalis and Its Application in Peanut Field[J]. Chinese Journal of Biological Control, 2021, 37(4): 732-739.

参考文献

[1] Chau A, Heinz K, Fred T, et al. Influences of fertilization on population abundance, distribution, and control of Frankliniella occidentalis on chrysanthemum[J]. Entomologia Experimental et Appicata, 2005, 17(1):17-39.
[2] 庞洪翠, 唐勇斌, 贾彦霞. 西花蓟马在不同辣椒品种上的实验种群生命表[J]. 植物保护, 2019, 5(3):134-137.
[3] 王圣印, 张安盛, 李丽莉, 等. 西花蓟马田间种群对常用杀虫剂的抗性现状及防治对策[J]. 昆虫学报, 2014, 57(5):621-630.
[4] Liu J N, Xu S G, Zhang Y Y, et al. Systematic analysis of gene expression in western flower thrip (Frankliniella occidentalis Pergande) before and after infection with impatiens necrotic spot virus (INSV)[J]. Agricultural Biotechnology, 2017, 6(5):28-31,46.
[5] 陈育华, 许燕, 李辉, 等. 潮汕地区花生主要病虫害及其综合防治技术[J]. 广东农业科学, 2009(3):105-107.
[6] Li D G, Shang X Y, Stuart R, et al. Field resistance to spinosad in western flower thrips Frankliniella occidentalis (Thysanoptera:Thripidae)[J]. Journal of Integrative Agriculture, 2016, 15(12):2803-2808.
[7] 陈雪林, 杜予州, 王建军. 西花蓟马抗药性研究进展[J]. 植物保护, 2011, 37(5):34-38.
[8] 黄鹏, 陈汉鑫, 姚锦爱, 等. 金龟子绿僵菌对石蒜绵粉蚧的室内毒力与防治效果[J]. 中国生物防治学报, 2019, 35(6):884-890.
[9] 葛文超, 杜广祖, 赵永鑫, 等. 西花蓟马高毒力虫生真菌筛选及其生防应用潜力的研究进展[J]. 生物安全学报, 2018, 27(4):240-248.
[10] Ansari M A, Brownbridge M, Shah F A, et al. Efficacy of entomopathogenic fungi against soil-dwelling life stages of western flower thrips, Frankliniella occidentalis, in plant-growing media)[J]. Entomologia Experimentalis et Applicata, 2008, 127(2):80-87.
[11] Mburu D M. Selection of promising fungal biological control agent of the western flower thrips Frankliniella occidentalis (Pergande)[J]. Letters in Applied Microbiology, 2018, 54:487-493.
[12] Maniania N K, Sithanantham S, Ekesi S, et al. A field trial of the entomogenous fungus Metarhizium anisopliae for control of onion thrips, Thrips tabaci[J]. Crop Protection, 2003, 22(3):553-559.
[13] 王兴民, 邵振芳, 王泽清, 等. 绿僵菌素与化学农药混配对西花蓟马的联合毒力[J]. 广东农业科学, 2015, 42(16):60-63.
[14] 徐蕾, 许国庆, 陈彦, 等. 近5年国内大豆、花生主要虫害的生物防治研究进展[J]. 中国植保导刊, 2014, 34(5):15-19.
[15] 曲明静, 刘爱娜, 曾庆朝, 等. 气象因子对青岛市花生田西花蓟马种群数量的影响[J]. 花生学报, 2019, 48(4):43-48.
[16] Wu S Y, Gao Y L, Xu X N, et al. Compatibility of Beauveria bassiana with Neoseiulus barkeri for Control of Frankliniella occidentalis[J]. Journal of Integrative Agriculture, 2015, 14(1):98-105.
[17] NY/T 1154.8-2007, 农药室内生物测定试验准则杀虫剂第8部分:滤纸药膜法[S]. 中华人民共和国农业部.
[18] GB/T 17980.85-2004, 田间药效试验准则.杀菌剂防治花生叶斑病[S]. 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会.
[19] 李保国. 金龟子绿僵菌的胞外酶与毒力相关性及应用技术研究[D]. 保定:河北农业大学, 2015.
[20] 何发林, 姜兴印, 尚佃龙, 等. 氯虫苯甲酰胺种子处理对花生地上及地下害虫的防治效果[J]. 植物保护学报, 2019, 46(1):239-248.
[21] 谷莉莉, 仇学平, 陈永明, 等. 几种生物农药对设施小白菜小菜蛾的防控效果[J]. 中国植保导刊, 2019, 39(12):76-78.
[22] Liu J N, Chen Z B, Yu L, et al. Effects of plant secondary metabolite nicotine on growth and development of Frankliniella occidentalis[J]. Plant Diseases and Pests, 2018, 9(2):21-24, 36.
[23] 万岩然, 何秉青, 苑广迪, 等. 北京和云南地区西花蓟马对多杀菌素类药剂产生抗药性[J]. 应用昆虫学报, 2016, 53(2):396-402.
[24] Vestergaard S, Gillespie A T, Butt T, et al. Pathogenicity of the hyphomycete fungi Verticillium lecanii and Metarhizium anisopliae to the western flower thrips, Frankliniella occidentalis[J]. Biocontrol Science and Technology, 1995, 5(2):185-192.
[25] Taro S, Michael B. Compatibility of foliage-dwelling predatory mites and mycoinsecticides, and their combined efficacy against western flower thrips Frankliniell aoccidentas[J]. Journal of Pest Science, 2018, 91(4):1291-1300.
[26] Pardey, Alex E B. Evaluation of chemical and biological insecticides to control Frankliniella occidentalis (Thysanoptera:Thripidae) in asparagus crops[J]. Revista Colombiana de Entomologa, 2009, 35(1):12-17.
[27] Se J, Lee S, Kim J, et al. Entomopathogenic Beauveria bassiana granules to control soil-dwelling stage of western flower thrips, Frankliniella occidentalis (Thysanoptera:Thripidae)[J]. BioControl, 2017, 62(5):639-648.
[28] Wu S, He Z, Wang E, et al. Application of Beauveria bassiana and Neoseiulus barkeri for improved control of Frankliniella occidental is in greenhouse cucumber[J]. Crop Protection, 2017, 96(96):83-87.
[29] 刘平. 巴氏新小绥螨的捕食功能反应及保护利用[D]. 兰州:甘肃农业大学, 2014.
[30] Li F M, Jun R Z, Tian T, et al. Biological control efficiency of Orius similis Zheng (Hemiptera:Anthocoridae) on Frankliniella occidentalis (Pergande) under different spatial and caged conditions[J]. Acta Ecologica Sinica, 2013, 33(22):7132-7139.
[31] 李宜儒, 李琼, 胡昌雄, 等. 吡虫啉对南方小花蝽捕食西花蓟马功能反应的影响[J]. 云南农业大学学报(自然科学), 2019, 34(4):590-596.
[32] 常金梅, 何衍彪, 赵燕龙, 等. 绿僵菌致病力的制约因素研究[J]. 安徽农业科学, 2010, 38(6):2815-2816.
[33] 蒋智林, 桂富荣, 刘万学, 等. 西花蓟马对寄主植物的危害及其影响机理研究进展[J]. 安徽农业科学, 2011, 39(19):11529-11530,11550.
[34] 农向群, 张英财, 王以燕. 国内外杀虫绿僵菌制剂的登记现状与剂型技术进展[J]. 植物保护学报, 2015, 42(5):702-714.
[35] 张秀霞, 赵忠范, 毛晓红, 等. 金龟子绿僵菌CQMa421与3种杀虫剂对瓜蚜的联合毒力[J]. 农药, 2020, 59(1):74-78.