茶尺蠖和灰茶尺蠖幼虫两种共有寄生蜂的鉴定

doi:10.16409/j.cnki.2095-039x.2022.01.003

中国生物防治学报 ›› 2023, Vol. 39 ›› Issue (1): 1-9.DOI: 10.16409/j.cnki.2095-039x.2022.01.003

• 研究论文 • 下一篇

茶尺蠖和灰茶尺蠖幼虫两种共有寄生蜂的鉴定

周孝贵¹, 唐璞², 吴琼², 郭华伟¹, 肖强¹, 陈学新²

1. 中国农业科学院茶叶研究所/农业农村部茶叶质量安全控制重点实验室, 杭州 310008;
2. 浙江大学昆虫科学研究所, 杭州 310058

收稿日期:2021-12-24 出版日期:2023-02-08 发布日期:2023-02-21
通讯作者: 肖强,研究员,E-mail:xqtea@vip.163.com;陈学新,博士,教授,E-mail:xxchen@zju.edu.cn
作者简介:周孝贵,硕士,助理研究员,E-mail:zxg@tricaas.com;唐璞,博士,副教授,E-mail:ptang@zju.edu.cn
基金资助:
国家重点研发计划（2019YFD1002100）；中国农业科学院茶叶研究所基本科研业务费（1610212019002）；农业基础性长期性科研工作（ZX09NK22）；中央高校基本科研业务费（2021FZZX001-31）

Identification of Two Common Larval Parasitic Wasps of Ectropis obliqua and Ectropis grisescens (Lepidoptera: Geometridae)

ZHOU Xiaogui¹, Tang Pu², Wu Qiong², GUO Huawei¹, XIAO Qiang¹, CHEN Xuexin²

1. Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture/Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
2. Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China

Received:2021-12-24 Online:2023-02-08 Published:2023-02-21

摘要/Abstract

摘要： 茶尺蠖Ectropis obliqua(Prout)及其近缘种灰茶尺蠖E.grisescens Warren是我国茶树上最为重要的两种食叶性害虫，茶尺蠖绒茧蜂Apanteles sp.和单白绵绒茧蜂Apanteles sp.是茶尺蠖Ectropis spp.幼虫期最重要的两种寄生性天敌昆虫。为了明确这两种绒茧蜂的物种名称及形态差别，同时探明这两种绒茧蜂是否是茶尺蠖和灰茶尺蠖的共有寄生蜂，本研究采集了我国8省22个地方的茶尺蠖或灰茶尺蠖幼虫，通过室内饲养获得两种绒茧蜂的标本，对标本进行形态鉴定；同时将两种绒茧蜂的COI基因序列与Bold数据库中已知物种进行比对。结果表明，从野生茶尺蠖和灰茶尺蠖幼虫中均饲养出之前报道的茶尺蠖绒茧蜂Apanteles sp.和单白绵绒茧蜂Apanteles sp.两种寄生蜂；形态鉴定和分子比对结果表明，这两种广义绒茧蜂Apanteles spp.实为尺蠖原绒茧蜂Protapanteles immunis(Haliday,1834)和单白绵副绒茧蜂Parapanteles hyposidrae(Wilkinson,1928)。本研究明确了40多年前国内报道的茶尺蠖幼虫期的两种绒茧蜂Apanteles spp.的物种名称和主要形态特征，确定了这两种绒茧蜂是茶尺蠖和灰茶尺蠖的共有寄生蜂，同时报道了一个中国新记录种尺蠖原绒茧蜂。研究结果为今后正确判定本地优势寄生蜂物种进而对茶尺蠖和灰茶尺蠖进行生物防治提供科学依据。

关键词: 尺蠖原绒茧蜂, 单白绵副绒茧蜂, 形态鉴定, DNA条形码, 中国新记录种

Abstract: Ectropis obliqua (Prout) and Ectropis grisescens Warren (Lepidopotera: Geometridae) are two of the most destructive chewing pests in China’s tea plantations. Two of the parasitic wasps, which previously reported as Apanteles spp. (Hymenoptera: Braconidae: Microgastrinae), can play important role in controlling Ectropis spp. during the larval stage in nature. In order to determine the species names and morphological differences of the two wasps, and to confirm whether these two species are common parasitic wasps of E. obliqua and E. grisescens, we identified the wasp specimens emerged from E. obliqua and E. grisescens that were collected from 22 locations in 8 provinces of China and reared in the laboratory, and compared the COI sequences of the voucher specimens with those of known species recorded in the BOLD database. The findings revealed that both parasitic wasps emerged from E. obliqua as well as E. grisescens during the larval stage. Based on morphological and molecular data, these two species of Apanteles spp. were identified as Protapanteles immunis (Haliday, 1834) and Parapanteles hyposidrae (Wilkinson, 1928). In conclusion, this study has confirmed the specific species names and morphological characteristics of the two wasps reported as Apanteles sp. in China more than 40 years ago, which are common larval parasitic wasps of E. obliqua and E. grisescens, and the P. immunis is recorded for the first time in China. These results provide a scientific basis for accurately identifying indigenous dominant parasitic wasps, which might help manage E. obliqua and E. grisescens.

Key words: Protapanteles immunis, Parapanteles hyposidrae, morphological identification, DNA barcoding, new record species for China

中图分类号:

S476.3

周孝贵, 唐璞, 吴琼, 郭华伟, 肖强, 陈学新. 茶尺蠖和灰茶尺蠖幼虫两种共有寄生蜂的鉴定[J]. 中国生物防治学报, 2023, 39(1): 1-9.

ZHOU Xiaogui, Tang Pu, Wu Qiong, GUO Huawei, XIAO Qiang, CHEN Xuexin. Identification of Two Common Larval Parasitic Wasps of Ectropis obliqua and Ectropis grisescens (Lepidoptera: Geometridae)[J]. Chinese Journal of Biological Control, 2023, 39(1): 1-9.

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茶尺蠖和灰茶尺蠖幼虫两种共有寄生蜂的鉴定

Identification of Two Common Larval Parasitic Wasps of Ectropis obliqua and Ectropis grisescens (Lepidoptera: Geometridae)

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