斜纹夜蛾取食Cry1Ca蛋白后中肠组织病理变化

doi:10.16409/j.cnki.2095-039x.2022.05.005

摘要/Abstract

摘要： Cry1Ca对斜纹夜蛾、甜菜夜蛾等多种鳞翅目害虫具有很高的杀虫活性，它在转多种Bt蛋白基因策略中，可用于扩大杀虫谱，并可用于当前Bt蛋白的抗性治理，是极具潜力蛋白之一。为明确Cry1Ca对斜纹夜蛾的作用机制，我们利用透射电镜观察了幼虫取食Cry1Ca后中肠组织的病理变化。结果表明斜纹夜蛾取食含Cry1Ca蛋白的饲料2 h后中肠组织开始表现明显的病理变化，且随取食时间的增加病变越来越严重。具体表现为：中肠细胞微绒毛肿胀、扭曲、断裂和脱落；细胞核拉长或畸形、核膜模糊、染色质不均匀或聚集；线粒体变形、空泡化和内脊模糊不清；内质网杂乱无章、肿胀变形和数量变少。研究结果可为更好地利用Cry1Ca防治斜纹夜蛾提供理论依据。

关键词: 斜纹夜蛾, Cry1Ca, 中肠组织, 病理变化

Abstract: Cry1Ca has high toxicity to Spodoptera exigua, Spodoptera litura and some other lepidoptera pests. The protein is of great potential to be applied in polygenic strategy of Bt (Bacillus thuringiensis) transgenic crops to enlarge the insecticidal spectrum and manage the insect resistance caused by the current Bt transgenic crop. The toxicity of Cry1Ca to S. litura was studied through observation of the pathological changes of midgut induced by Cry1Ca using transmission electron microscope. The results indicated that the midgut tissues were characterized by obvious pathological changes when the insects were fed Cry1Ca for 2 h, and the pathological changes became ever more obvious with the extension of feeding duration. The main symptoms included swollen, distorted, fractured, and dropped off cell microvilli; stretched or deformed cell nucleus, blurred boundary of karyotheca in cell nucleus, and uneven or gathered chromatins; deformed, cavitated or ridge-blurred mitochondria; and swollen, disordered and reduced endoplasimic reticulum. These results may facilitate the application of Cry1Ca as an important insecticidal protein in the Bt transgenic crops in the future.

Key words: Spodoptera litura, Cry1Ca protein, midgut tissues, pathological changes

中图分类号:

S476.1

姚雪, 刘臣, 段云鹏, 魏纪珍, 梁革梅. 斜纹夜蛾取食Cry1Ca蛋白后中肠组织病理变化[J]. 中国生物防治学报, 2022, 38(3): 760-766.

YAO Xue, LIU Chen, DUAN Yunpeng, WEI Jizhen, LIANG Gemei. Pathological Changes in Larval Midgut Tissues of Spodoptera litura Feeding on Cry1Ca Protein[J]. Chinese Journal of Biological Control, 2022, 38(3): 760-766.

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