Explore sip Gene from Bt Strain DQ89 and Its Insecticidal Activity against Colaphellus bowringi Baly

doi:10.16409/j.cnki.2095-039x.2015.04.023

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Explore sip Gene from Bt Strain DQ89 and Its Insecticidal Activity against Colaphellus bowringi Baly

ZHANG Jinbo¹, LI Haitao^1,2, LIU Rongmei¹, SHU Changlong², GAO Jiguo¹

1. College of Life Science, Northeast Agricultural University, Harbin 150030, China;2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2014-12-03 Revised:1900-01-01 Online:2015-08-08 Published:2015-08-08

Abstract

Abstract: Bacillus thuringiensis insecticidal crystal proteins (ICPs), vegetative insecticidal protein (VIP) and secreted insecticidal protein (SIP) were widely used as a kind of safe bio-insecticides. ICP and VIP have been reported widely, while few studies have concerned on SIP. In this study, PCR method was used to clone and identify the sip gene from Bt strains isolated from different environments. A sip gene was obtained from the strain DQ89, which is of 1188 bp and encodes 395 amino acids, showing a 87% similarity of amino acid residues with the registered Sip1A protein. The nucleotide sequencing was submitted to GenBank with an Accession No. of KP114614. The protein expressed in E. coli showed an insecticidal activity against Colaphellus bowringi Baly, with LC₅₀ of 1.542 μg/mL.

CLC Number:

S476.11

ZHANG Jinbo¹, LI Haitao^1,2, LIU Rongmei¹, SHU Changlong², GAO Jiguo¹. Explore sip Gene from Bt Strain DQ89 and Its Insecticidal Activity against Colaphellus bowringi Baly[J]. , DOI: 10.16409/j.cnki.2095-039x.2015.04.023 .

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Explore sip Gene from Bt Strain DQ89 and Its Insecticidal Activity against Colaphellus bowringi Baly

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