Abstract: To establish a whole season biological control technology for broad bean diseases and pests to promote the production of organic broad beans in Qinghai, we determined the biological activities of 9 biopesticides on Aphis craccivora Koch (Hemiptera: Aphididae), broad bean chocolate spot and fusarium wilt using laboratory and field tests. The laboratory bioassay tests showed that the four biopesticides had significant activities against A. craccivora. Among them, 0.3% matrine AS was the best, with LC₅₀ of 24 h and 48 h of 7.33 mg/L and 5.66 mg/L, respectively. The field tests showed that 0.3% matrine AS and 0.5% veratrine soluble concentrate (SL) had significant control on A. craccivora, with the control efficacy of 76.00%-83.00% at 7 d after application of 500-fold dilution. The mixture of 10% berberine wettable powders (WP) and Bacillus subtilis (100 billion spores/g) WP had a synergistic effect on the control efficacy of broad bean chocolate spot, with the control efficacy of 73.98%-77.79% at 14 d after application of the 500.0 g/hm²preparation. Paenibacillus polymyxa (1 billion CFU/g) WP and Trichoderma harzianum (≥200 million live spores/g) WP were more effective against broad bean fusarium wilt than the other two biopesticides, with the control efficacy of 74.23% and 71.01% at 14 d after application, respectively. Therefore, 0.3% matrine AS, 0.5% veratrine SL, 10% berberine WP, Bacillus subtilis WP, Paenibacillus polymyxa WP and Trichoderma harzianum WP have excellent control on the three main diseases and pests of broad bean. The results are of significance for whole season biological control technology for organic, green and safe production of broad bean in Qinghai province.

Key words: biological pesticides, broad bean, Aphis craccivora, chocolate spot, fusarium wilt