%0 Journal Article %A ZHAO Weisong %A GUO Qinggang %A ZHANG Xiaoyun %A WANG Peipei %A SU Zhenhe %A HU Qing %A LU Xiuyun %A MA Ping %A LI Shezeng %T Development of Microbial Agent Bacillus amyloliquefaciens PHODG36 and Its Effect on Disease Control and Yield Increase of Potato %D 2020 %R 10.16409/j.cnki.2095-039x.2020.03.014 %J Chinese Journal of Biological Control %P 381-387 %V 36 %N 3 %X Bacillus amyloliquefaciens PHODG36 is a multifunctional strain which has significant control efficiency on diseases and growth-promotion effect on plants. In this study, microbial agent with different spore concentrations was prepared using strain PHODG36 raw powder as active ingredient and talc powder as filler. Pot experiments were conducted to investigate potato seedling emergence after seed dressing, and field plot experiments were carried out to investigate the effect of microbial agent PHODG36 on control efficacy of potato Verticillium wilt and potato yield in northern and southern Hebei Province. The results showed that microbial agent PHODG36 had a light yellow appearance and no agglomeration. The microbial agent had an average particle size of (38.25±0.46) μm and pH value of (6.65±0.15). There was no caking and sticking after storage for the agent, and the concentrations were ranged from 1.5×109 to 2.0×1010 CFU/g. Compared with control and chemical treatment, there was no significant difference on the corrected emergence rate among different concentrations of microbial agent PHODG36 after seed dressing, but the corrected emergence rate decreased with the increase of concentration. Field experiments in different areas showed that the control efficacy on disease index of Verticillium wilt was 43.08% to 58.46%, and the yield increased from 8.87% to 30.37% for susceptible varieties, 1.85%~9.0% for resistant varieties, when the concentration of microbial agent PHODG36 were ranged from 1.5×109 to 1.0×1010 CFU/g. Comprehensive analysis showed that the application of microbial agent PHODG36 at 1.5×109 to 1.0×1010 CFU/g could effectively reduce the incidence and disease index of potato Verticillium wilt, save the cost of investing, and significantly increase production. The results indicated that green products were provided for control of Verticillium wilt, which laid a foundation for the further extensive application of microbial agent PHODG36. %U http://www.zgswfz.com.cn/EN/10.16409/j.cnki.2095-039x.2020.03.014