%0 Journal Article
%A DU Chanjuan
%A YANG Di
%A PAN Lianfu
%A YE Yunfeng
%A ZHANG Jin
%A FU Gang
%T Bio-Fertilizer Optimization and Its Control Effect on Banana Fusarium Wilt
%D 2020
%R 10.16409/j.cnki.2095-039x.2020.03.016
%J Chinese Journal of Biological Control
%P 396-404
%V 36
%N 3
%X In order to improve the living conditions of antagonistic fungus in the fertilizer and improve control effect of the bio-fertilizer on banana Fusarium wilt, a variety of factors related to the growth of antagonistic strain were optimized based on the GFP-labeled strain Thichoderma harzianum GFP-gz-2. The effects of the bio-fertilizer on disease control and growth promotion were evaluated before and after optimization, respectively. The results showed that glutinous rice flour was the best one among the 7 carbon sources for the growth of strain GFP-gz-2. The colony diameter and sporulation of strain GFP-gz-2 reached the maximum when the content of glutinous rice flour was 35 g/L. Ca2+ was the best one in the 6 metal ions for the growth of strain GFP-gz-2. The results of multi-factor orthogonal optimization showed that the best conditions for the growth of strain GFP-gz-2 in the bio-fertilizer were listed as follows:C/N ratio of organic fertilizer was 30:1, concentration of Ca2+was 5%, and the inoculation amount of the strain was 2.0×106 spore/g. Under these conditions, the colonization amount of strain GFP-gz-2 in the bio-fertilizer was 1.033×108 spore/g. The control effect of optimized bio-fertilizer on banana Fusarium wilt reached to 84.75%, 15.26% higher than that before optimization. The optimized bio-fertilizer increased the plant height, stem girth, fresh weight and dry weight of banana significantly, which were 23.35%, 22.82%, 68.78% and 50.17% higher than that before optimization, respectively.
%U http://www.zgswfz.com.cn/EN/10.16409/j.cnki.2095-039x.2020.03.016