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Comparative Transcriptomic Profiles Revealed JA- and SA- Regulated Soft Rot Disease Resistance by Bacillus velezensis BCP6 in Amorphophallus konjac ZHANG Meng, CHEN Sen, TANG Dengguo, HUANG Yan, LONG Zhijian, WANG Boya, HU Shanglian, CAO Ying Chinese Journal of Biological Control    2024, 40 (6): 1375-1385.   DOI: 10.16409/j.cnki.2095-039x.2024.02.060 Abstract （251）      PDF（pc） （4362KB）（7373）       Knowledge map    Save The application of Bacillus velezensis has been proven to significantly enhance the resistance to soft rot in Amorphophallus konjac, yet its precise biological control mechanisms remain unclear. This study explored the effects of B. velezensis BCP6 on the defense pathways of A. konjac in response to P. aroidearum MY11 infection through comparative transcriptome analysis. Differential expression analysis, gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA) revealed that BCP6 application upregulated pathways related to jasmonic acid (JA), reactive oxygen species, and cell wall synthesis, thereby activating the basic defense system of A. konjac. This immune activation facilitated the rapid initiation of defense responses when subjected to subsequent P. aroidearum infection by enhancing the JA-mediated induced systemic resistance (ISR) and SA-dependent systemic acquired resistance (SAR) signaling pathways. Simultaneously, a significant increase in the expression of genes involved in cell wall formation and pathogenesis-related proteins was observed, effectively enhancing resistance to soft rot. Meanwhile, exogenous JA treatment was found to directly stimulate the expression of cell wall-related genes, namely CESA2 and CSI1, while SA significantly induced the expression of SA-dependent defense genes TLP and E13C. The results provided a theoretical basis for biological control and disease-resistant breeding of konjac. Reference | Related Articles | Metrics | Comments（0）

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Research Progress on Green and Sustainable Management Technologies for Spodoptera frugiperda WEI Lei, NIE Ying, LI Yongjun Chinese Journal of Biological Control    2025, 41 (1): 207-219.   DOI: 10.16409/j.cnki.2095-039x.2024.07.004 Abstract （489）      PDF（pc） （530KB）（3868）       Knowledge map    Save The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), is a major worldwide disastrous pest. Since its invasion into China in 2019, the implementation of emergency chemical control measures yielded positive outcomes. However, the occurrence of S. frugiperda in China has now become common and year-round, necessitating a long-term approach for its management. Throughout the history of global pest control, reliance on chemical agents has invariably led to the development of pest resistance. Incidences of pesticide resistance in S. frugiperda have been reported in certain regions of China. Therefore, to mitigate the adverse effects of chemical pesticides and the risk of pesticide resistance, China's management strategies must transition towards green, efficient, precise, and sustainable approaches. On the basis of the regional control strategy and unified management strategy, the Ministry of Agriculture and Rural Affairs has advocated a management framework emphasizing biological control as the focal point, ecological and agricultural control as the foundation, and chemical control as the last resort. This article elaborates on recent technological advancements both domestically and internationally in biological control, ecological and agricultural control methods, with an emphasis on biological control. Additionally, it proposes future research directions and ideas to overcome existing challenges, aiming to provide guidance for the development of green management technologies in China. Reference | Related Articles | Metrics | Comments（0）

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Research Progress and Prospect of DNA Barcoding in Parasitoid Wasps SUN Xingxing, JIANG Yingjie, WANG Fan, ZHANG Lisheng Chinese Journal of Biological Control    2025, 41 (2): 473-491.   DOI: 10.16409/j.cnki.2095-039x.2024.07.007 Abstract （205）      PDF（pc） （530KB）（2885）       Knowledge map    Save Parasitoid wasps play a crucial role in the biological control of agricultural and forestry pests, such as lepidoptera and hemiptera. They have significant potential for development and application in biological control strategies. DNA barcoding, a modern molecular technology widely used in animal and plant biology, ecology, and other fields, has emerged as an important tool for classifying parasitic wasps and conducting ecological research. This paper reviews relevant literature to summarize the primary DNA barcodes and primers utilized for parasitoid wasps. It also discusses the applications of DNA barcoding in taxonomic identification, detection of new or cryptic species, analysis of food web relationships, and phylogenetic research on parasitic wasps. Furthermore, this paper analyzes current challenges faced by DNA barcoding research on parasitic wasps while proposing prospects for key issues such as selecting appropriate DNA barcodes, screening suitable phylogenetic models, and utilizing high-throughput sequencing technology. The aim is to provide theoretical references for resource exploration of parasitic wasps, optimization of biological control strategies, and advancements in evolutionary biology. Reference | Related Articles | Metrics | Comments（0）

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Research Progress on Damage Characteristics and Parasitoids of Important Pests in Peach SUN Xingxing, WANG Fan, JIANG Yingjie, ZHANG Lisheng Chinese Journal of Biological Control    2025, 41 (1): 231-250.   DOI: 10.16409/j.cnki.2095-039x.2024.03.018 Abstract （208）      PDF（pc） （668KB）（2170）       Knowledge map    Save Prunus persica is one of the vital fruit resources in China, with their growth, development, and fruit yield significantly affected by a wide range of pests. Parasitoid wasps play a crucial role as key biological control agents in effectively managing pests such as peach aphids, peach borer moths, and peach fruit borers. This article provides a comprehensive review of published literature, focusing on the important pests of peach trees and their damage characteristics, the progress in research on related parasitoid wasp resources, and the application of these wasps in integrated pest management. The review elaborated on 21 pests, including mites, that have a significant economic impact on peach trees, and listed 194 species of parasitoid wasps across 20 families that play a pivotal role in the biological control of peach tree pests. Additionally, the article summarized current issues in research on parasitoid wasps for peach tree pests and proposed future research directions, aiming to offer deeper theoretical support for improving pest management strategies and protecting biodiversity in peach orchards. Reference | Related Articles | Metrics | Comments（0）

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Advance in Push-Pull Strategy for Control of the Asian Citrus Psyllid Diaphorina citri LIN Wenhan, WANG Jing, ZHOU Yutong, SANG Wen, QIU Baoli Chinese Journal of Biological Control    2025, 41 (1): 220-230.   DOI: 10.16409/j.cnki.2095-039x.2024.07.002 Abstract （248）      PDF（pc） （488KB）（2120）       Knowledge map    Save Citrus is one of the important economic crops in the world. With the expansion of production scale, pest control has become a bottleneck restricting its development. Diaphorina citri is the most serious worldwide pest among numerous insects that harm citrus. Its adults and nymphs can not only directly stab and damage rutaceae plants such as citrus and lemon, but also transmit citrus Huanglongbing (HLB) as the only natural vector. The ‘Push-pull strategy’, with the advantages of economy, convenience, high-efficiency and green, is crucial in the ecological regulation and management of pests. Popularizing the ‘push-pull’ strategy could contribute to controlling the citrus psyllid and promoting the green development of the citrus industry. This review summarized the current progress from components of push and pull. It is expected that the development trends of push-pull strategy will provide theoretical reference for the economical, convenient and effective control of citrus psyllid and accelerate the healthy and sustainable development of citrus industry. Reference | Related Articles | Metrics | Comments（0）

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Research Advances in Diapause of Predatory Stink Bugs (Hemiptera) XU Bowen, LI Yuyan, HE Weiwei, XUE Chuanzhen, SHEN Zhongjian, MAO Jianjun, ZHANG Lisheng, WU Huihui Chinese Journal of Biological Control    2025, 41 (1): 193-206.   DOI: 10.16409/j.cnki.2095-039x.2024.03.017 Abstract （229）      PDF（pc） （530KB）（1612）       Knowledge map    Save The predatory stink bugs are economically important natural enemy insects and are effective in the biological control of agricultural and forestry pests. Diapause is a physiological adaptation strategy that insects can enter a developmental arrest state in response to unfavorable environmental conditions. Many species of predatory stink bugs enter diapause as adults or eggs. Understanding the characteristics and the regulation mechanisms of diapause in predatory stink bugs not only helps to decipher the environmental adaptation mechanism of insect development, but also can prolong the storage period of natural enemies, promote their year round mass production and improve the control efficiency by manipulating the diapause process. It is of great significance to promote the large-scale mass production, conservation and utilization of natural enemy insects. In this review, the diapause characteristics of 27 species of predatory stink bugs were summarized. The biological, ecological and physiological features during diapause of these predatory stink bugs were analyzed. In addition, the molecular regulation mechanism of diapause and the post-diapause developmental biology of the predatory stink bugs were also reviewed. Finally, the application prospect of diapause in the mass rearing of predatory stink bugs and their use in biological control was discussed. This review provides an important reference for in-depth research on the diapause manipulation technique and diapause mechanism in predatory stink bugs, and for promotion of their mass production, storage and application. Reference | Related Articles | Metrics | Comments（0）

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Preparation of Wettable Powder Formulation of Trichoderma harzianum M-17 Chlamydospores and Its Field Control Effect on Potato Dry Rot WANG Xigang, GUO Chengjin, JIAO Yang, ZHAO Pei, TIAN Jing, ZHANG Lirong, SHEN Ruiqing Chinese Journal of Biological Control    2024, 40 (6): 1319-1330.   DOI: 10.16409/j.cnki.2095-039x.2024.02.061 Abstract （350）      PDF（pc） （576KB）（1598）       Knowledge map    Save To development an efficient biocontrol antimicrobial agent of potato dry rot and prolong the shelf of the product, this study utilized Trichoderma harzianum M-17 as the primary research object. Single factor selection and formulation optimization were used to obtain the wettable powder (WP). The optimal formulation of WP was chlamydospore powder (20%), attapulgite (68%) as carrier, sodium dodecyl sulfonate (4%) as wetting agent, sodium carboxylcellulose (7%) as dispersion, and ascorbic acid (1%) as UV protective agent. The chlamydospore content of this WP was 3.1×108 CFU/g, the wetting time was 56.5 s, the spore suspension rate was 82.27%, pH 6.79, the moisture content was 2.26%, the ratio transiting standard test sieve with 74 µm was 98%. All indicators meet the national standards. The results of field experiments showed that T. harzianum M-17 chlamydospore wettable powder could promote the growth of potato plants and indicated a good control effect on potato dry rot, with a control efficacy of 70.32% and a yield increase rate of 19.03%. This study lays a technical foundation for the development of T. harzianum M-17 chlamydospore wettable powder into a microbial fungicide for the control of potato soil-borne diseases. Reference | Related Articles | Metrics | Comments（0）

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Screening of Endophytic Fungi of Andrographis paniculata Resistant to Ralstonia solanacearum Infecting Peanuts and Study of Their Inhibitory Components HUANG Jin, HU Jinwen, DING Mei, YANG Fan, LUO Wenrong, DU Qin Chinese Journal of Biological Control    2025, 41 (2): 396-410.   DOI: 10.16409/j.cnki.2095-039x.2025.02.028 Abstract （345）      PDF（pc） （5856KB）（1570）       Knowledge map    Save To identify potential biocontrol strains against peanut bacterial wilt caused by Ralstonia solanacearum, we screened endophytic fungi of Andrographis paniculata leaves using the Oxford cup inhibition method. A strain with strong inhibitory activity against R. solanacearum was isolated and identified as Colletotrichum sp. CXL-11 based on morphological characteristics and 16S rDNA phylogenetic analysis. We further investigated its antibacterial mechanism through co-culture experiments and analyzed the antifungal components in its fermentation extract using LC-MS metabolomics. The bioactivity of selected metabolites against R. solanacearum was also validated. The results showed that the fermentation extract of CXL-11 produced an inhibition zone of 19.08 mm against R. solanacearum, with a minimum inhibitory concentration (MIC) of 0.625 mg/mL. The extract disrupted the membrane integrity of R. solanacearum, causing surface shrinkage and deformation of bacterial cells, and induced oxidative stress through the accumulation of reactive oxygen species. Metabolomic analysis revealed that the primary antifungal components were polyketides and coumarins, including isogentisin, bergapten, scopoletin, scopolin, and esculetin, all of which exhibited inhibitory effects against R. solanacearum. The biosynthesis pathway of cofactors was identified as a key mechanism underlying the antibacterial activity of CXL-11. In conclusion, the endophytic fungus Colletotrichum sp. CXL-11 demonstrates significant potential as a biocontrol agent for peanut bacterial wilt. This study provides theoretical support for the development of natural antimicrobial agents and highlights the promising application of CXL-11 in sustainable disease management. Reference | Related Articles | Metrics | Comments（0）

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Biocontrol Effects of Bacillus amyloliquefaciens HN11 in Combination with Tobacco Waste Organic Fertilizers on Strawberry Root Rot LIU Miaojiao, LIN Sukun, LIU Rong, ZHANG Junhui, LI Lin, YI Ting, XU Hanhong, ZENG Lingda Chinese Journal of Biological Control    2025, 41 (1): 143-155.   DOI: 10.16409/j.cnki.2095-039x.2025.02.009 Abstract （167）      PDF（pc） （3844KB）（1513）       Knowledge map    Save This study investigates an environmentally friendly approach to control strawberry root rot using biocontrol agents. Laboratory experiments revealed that B. amyloliquefaciens HN11 exhibits antagonistic activity against various soil-borne pathogens, particularly disrupting the mycelial structure of F. oxysporum, the primary pathogen causing strawberry root rot, and interfering with its physiological metabolism. In pot experiments, the combination of HN11 and tobacco waste organic fertilizer achieved a disease control efficacy of 75.00% against strawberry root rot. The organic fertilizer enhanced the colonization ability of HN11, maintaining its population at 104-105 CFU/g in soil and rhizosphere even 30 days after application. Field trials further demonstrated that applying HN11 via root drench at 75 L/h2 in conjunction with 6000 kg/hm2 of tobacco waste organic fertilizer resulted in the best disease control efficacy of 73.85%, while also promoting strawberry growth. High-throughput sequencing analysis indicated that this combined application optimized the soil microbial community structure, increasing the relative abundance of beneficial genera such as Bacillus and Pseudomonas, while reducing the relative abundance of Ascomycota. Overall, the integration of HN11 with tobacco waste organic fertilizer offers a promising green strategy for the management of strawberry root rot. Reference | Related Articles | Metrics | Comments（0）

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Screening of the Inhibitory Effect of Antagonistic Bacteria Compounded with Herbal Extracts on Sphaeropsis sapinea NIU Anqi, ZHOU Deming, MIAO Xinyu, CHEN Xinggang, ZHOU Guoying, LIU Junang Chinese Journal of Biological Control    2024, 40 (6): 1355-1365.   DOI: 10.16409/j.cnki.2095-039x.2024.02.069 Abstract （152）      PDF（pc） （4439KB）（1444）       Knowledge map    Save Pine wilt is a worldwide pine disease caused by Sphaeropsis sapinea,The main control methods are chemical control and biological control. Biological control is favored because it is based on nature and has the inherent advantages of green environmental protection. In order to screen effective antagonistic microorganisms, a strain of Bacillus amyloliquefaciens CSUFT-CSBA69 was selected from rhizosphere soil of Pinus massoniana. The inhibition rate was 76.18%. The crude extracts of traditional Chinese medicine were tested and their toxicity was determined. The results showed that Coptis Chinensis had the best inhibitory effect. The EC50 value was 0.033 mg/mL, the MIC was 0.3125 mg/mL, the MBC was 0.625 mg/mL, and the bacteriostatic effect was still present when the concentration decreased to 0.0097 mg/mL. In order to solve the realistic problem of the unstable effect of a single biocontrol factor in production practice and meet the demand of reducing the cost of biocontrol agents, this study adopted the compound method to explore the synergistic control effect of strain CSUFT-CSBA69 and Coptis chinensis low-concentration extract on S. sapinea. The bacterial inhibition was 97.45% when the Coptis chinensis was 0.05mg/mL and the inoculated amount of aseptic filtrate was 10%. The study demonstrated that the extract of Coptis Chinensis has a good inhibitory effect on pathogenic bacteria and could be combined with biocontrol bacteria. This study provided a new idea for the preparation of bio-derived pesticides. Reference | Related Articles | Metrics | Comments（0）

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Isolation and Identification of Bacillus subtilis ZF517 and Its Control Effect on Potato Common Scab WEI Xinchen, LIU Yi, YANG Ruwei, DUAN Guangrong, XIE Xuewen, SHI Yanxia, CHAI Ali, LI Baoju, LI lei Chinese Journal of Biological Control    2025, 41 (2): 411-422.   DOI: 10.16409/j.cnki.2095-039x.2025.02.020 Abstract （236）      PDF（pc） （3392KB）（1223）       Knowledge map    Save Potato common scab, a severe soilborne disease caused by Streptomyces scabies, has become increasingly prevalent in major potato-producing regions in China, causing significant yield and quality losses. However, effective and environmentally friendly control strategies remain limited. In this study, a biocontrol strain, ZF517, exhibiting strong antagonistic activity against S. scabies, was isolated. Based on morphological characteristics, physiological and biochemical tests, and multi-gene phylogenetic analysis, ZF517 was identified as Bacillus subtilis. Antagonistic spectrum analysis revealed that ZF517 exhibited potent inhibitory effects against six pathogenic fungi and three pathogenic bacteria. Biocontrol-related traits analysis showed that ZF517 secretes proteases and siderophores and harbours biosynthetic genes for Surfactin, Macrolactin, Fengycin, Difficidin, Bacillibactin, Bacillaene, Bacilysin, and Iturin. Moreover, the fermentation broth of ZF517 significantly inhibited the mycelial growth of S. scabies. A radish seedling assay confirmed that the fermentation broth effectively suppressed S. scabies infection, achieving a biocontrol efficacy of 63.94%. In pot experiments, ZF517 exhibited a biocontrol efficacy of 58.07% against potato common scab, significantly surpassing the efficacy of the chemical control treatment (20% thiazole zinc suspension concentrate). These findings indicate that ZF517 holds great potential as a biocontrol agent for managing potato common scab. Reference | Related Articles | Metrics | Comments（0）

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Sub-lethal Effect of Chlorantraniliprole on Predation Function of Harmonia axyridis HUANGFU Jiayi, LI Ping, GAO Dongmei, GUO Xiao Chinese Journal of Biological Control    2024, 40 (6): 1398-1405.   DOI: 10.16409/j.cnki.2095-039x.2024.03.004 Abstract （210）      PDF（pc） （1044KB）（1170）       Knowledge map    Save To identify the toxicity of chlorantraniliprole against Harmonia axyridis and how the insecticide impacts the natural enemy’s predation ability, insecticide-impregnated filter method was used to determine non-target toxicity of chlorantraniliprole, and its predation ability on Spodoptera frugiperda was also evaluated by predator-prey functional response model. The results showed that chlorantraniliprole induced toxic effect in H. axyridis (2nd instar larvae), with LC50(25.216 mg/L). Sub-lethal concentration of chlorantraniliprole would not change the type of functional response, which was still Holling II, but the model parameters changed. After treatment with sub-lethal concentration LC10, the attack rates of 4thinstar larvae, female and male adults respectively decreased by 18.61%, 28.03%, and 10.41%, the handling time respectively increased by 61.54%, 23.08%, and 25.00%, and the maximum number of preys consumed per day respectively decreased by 49.62%, 41.52%, and 28.33%. After treatment with sub-lethal concentration LC20, the attack rate respectively decreased by 21.99%, 26.67%, and 14.30%, the handling time respectively increased by 107.69%, 76.92%, and 91.67%, and the maximum number of preys consumed per day respectively decreased by 62.44%, 58.56%, and 55.28%. The results showed that chlorantraniliprole exposure would significantly impact the predator-prey functional response of H. axyridis, with a greater effect on the 4th instar larvae. The results provided data support for the screening of chemical insecticides compatible with natural enemies and helped H. axyridis work better. Reference | Related Articles | Metrics | Comments（0）

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Effect of Bacillus velezensis EEAM 10B on Peanut Southern Blight and Whole Genome Sequencing Analysis XIE Jiufeng, CHEN Mengxiao, WANG Bo, PEI Yaxin, ZHANG Jiran, CHEN Hongge, YANG Sen Chinese Journal of Biological Control    2025, 41 (2): 347-361.   DOI: 10.16409/j.cnki.2095-039x.2025.02.023 Abstract （195）      PDF（pc） （6141KB）（1112）       Knowledge map    Save To explore novel biocontrol microbial resources, a strain of Bacillus velezensis, designated as EEAM 10B, was isolated and identified from the surface of black soldier fly (Hermetia illucens) eggs. This study investigated its antagonistic effects and mechanisms against Sclerotium rolfsii Sacc, the pathogen of peanut southern blight. Peanut southern blight is a serious soil-borne fungal disease caused by Sclerotium rolfsii Sacc.Antagonism assays demonstrated that the fermentation broth, supernatant, and volatile organic compounds (VOCs) of EEAM 10B significantly inhibited the mycelial growth of peanut southern blight with the fermentation broth exhibiting the strongest suppression. Pot experiments revealed that EEAM 10B treatment notably reduced the disease index of infected peanut seedlings and exhibited plant growth-promoting effects. The optimal biocontrol efficacy (65.80%) was achieved when peanut plants were sprayed with EEAM 10B fermentation broth two days prior to pathogen inoculation. Whole-genome sequencing of EEAM 10B revealed a genome size of 3,929,786 bp with 1089.2×sequencing depth and a GC content of 46.5%. Functional annotation identified key genes encoding enzymes for fungal cell wall degradation (e.g., chitinases and β-1,3-glucanases) and secondary metabolite gene clusters responsible for antifungal compounds (e.g., surfactin, fengycin, and bacillibactin). Additionally, genes associated with plant growth promotion (e.g., indole-3-acetic acid synthesis) and pathogen antagonism were detected, suggesting their critical roles in suppressing peanut southern blight. These findings highlight the potential of EEAM 10B as a biocontrol agent and provide a foundation for developing novel antimicrobial formulations. Reference | Related Articles | Metrics | Comments（0）

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Synergistic Effect of Compound Seed Coating Containing Biocontrol Bacteria ZF516 and Chemical Agent on the Control of Cowpea Fusarium Root Rot ZHAO Zixuan, SHI Bin, LANG Junying, JIANG Tao, YANG Jingjing, FAN Tengfei, XIE Xuewen, SHI Yanxia, CHAI Ali, LI Baoju, LI Lei Chinese Journal of Biological Control    2025, 41 (1): 165-176.   DOI: 10.16409/j.cnki.2095-039x.2025.02.010 Abstract （206）      PDF（pc） （3446KB）（1106）       Knowledge map    Save In order to obtain biocontrol bacteria with remarkable antagonistic effects against cowpea root rot, in this study, the dural culture method was utilized to isolate a biocontrol strain, ZF516, which exhibited significant inhibitory effects on the complex pathogenic bacteria of cowpea root rot. Subsequently, the morphological characteristics of this strain were analyzed, and the 16S rRNA, gyrA, and rpoA gene sequences were compared and analyzed to clarify the taxonomic status of strain ZF516. The biosafety and root rot control efficacy of cowpea were evaluated through compatibility and compounding ratio screening of ZF516 with 23% imidacloprid·fludioxonil·difenoconazole and 29% thiamethoxam·fludioxonil·metalaxyl-M, respectively. The results indicated that the inhibition rates of strain ZF516 against Fusarium solani, F. oxysporum, and F. proliferatum were 71.83%, 66.31%, and 73.73%. The strain ZF516 was identificated as Bacillus velezensis. After formulating Bacillus velezensis ZF516 and 23% imidacloprid- thiamethoxam-benomyl at a volume ratio of 7:3, the emergence rate of cowpea seeds treated with this combined seed coating was significantly higher compared to untreated seeds and seeds treated with each agent individually. Additionally, the compound of Bacillus velezensis ZF516 with 23% imidacloprid·thiamethoxam·benomyl suspension seed coating agent showed a synergistic effect on the control of cowpea root rot, achieving a control efficiency of up to 67.56%, which surpassed the efficacy observed with the chemical seed coating agent alone. In this study, a strain of Bacillus velezensis ZF516 was isolated and characterized. ZF516 exhibited significant disease resistance against cowpea root rot, both as a standalone treatment and in combination with chemical agents. This study provides essential strain resources and integrated control strategies for the development of biological seed coating agents aimed at managing cowpea root rot. Reference | Related Articles | Metrics | Comments（0）

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Exogenous Salicylic Acid Regulates Root Metabolism in Panax notoginseng and Influences the Incidence of Root Rot Disease CHEN Shengqian, WANG Zhengping, SHI Yuyu, SHANG Zhaoyi, ZHU Shusheng, YANG Min Chinese Journal of Biological Control    2025, 41 (1): 177-185.   DOI: 10.16409/j.cnki.2095-039x.2025.02.011 Abstract （278）      PDF（pc） （593KB）（1075）       Knowledge map    Save The study investigated the effects of exogenous salicylic acid (SA) on root metabolism of Panax notoginseng, explored how differentially expressed metabolites influenced the growth of the main root rot pathogens of P. notoginseng, aiming to provide novel ideas for the ecological prevention and control of P. notoginseng root rot. Different concentrations of SA were sprayed on the leaves of P. notoginseng under continuous cropping conditions to screen the optimal SA concentration for reducing root rot disease. Gas chromatography-mass spectrometry (GC-MS) was then used to analyze the changes in metabolites in the fibrous roots of P. notoginseng at the optimal SA concentration, and the effects of significantly changed metabolites on the growth of P. notoginseng root rot pathogens were further studied. The results showed that compared with the control, exogenous application of 280.00 μg/mL SA could significantly increase the seedling survival rate of P. notoginseng in continuous cropping soil, and significantly promote the growth of root length and biomass. Metabolomics analysis revealed that the metabolites in the fibrous roots of P. notoginseng mainly included nine types of substances, such as organic acids, amino acids and their derivatives, sugars and their derivatives. After exogenous application of SA, the sugar metabolism and fatty acid metabolism of P. notoginseng roots changed significantly, and the contents of D-(+)-trehalose and linoleic acid decreased significantly. Further studies showed that these two significantly down-regulated metabolites could significantly promote the growth of the main root rot fungi within a certain concentration range. The results of this study provide a theoretical basis and technical support for the ecological prevention and control of P. notoginseng root rot. Reference | Related Articles | Metrics | Comments（0）

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A Comparative Genomics Analysis of the Biological Invasion Characteristics of Cydia pomonella TAO Shaomin, HE Shitian, FAN Yueyuan, YIN Chuanlin, YU Xiaoping Chinese Journal of Biological Control    2025, 41 (3): 721-732.   DOI: 10.16409/j.cnki.2095-039x.2024.11.007 Abstract （115）      PDF（pc） （3598KB）（1071）       Knowledge map    Save The codling moth (Cydia pomonella), a globally invasive pest affecting fruit trees, has now spread to 195 districts and counties across nine provinces and municipalities in China, which poses a significant threat to the apple, pear, and other fruit tree industries. In recent years, the advent of comparative genomics has offered novel perspectives and methodologies for investigating the genomic evolution and adaptive mechanisms of invasive species. In this study, we selected seven globally key invasive insect species, including the codling moth (Cydia pomonella), and seven closely related non-invasive insect species. We identified 2,324 single-copy orthologous genes, among which 110 genes exhibited significantly higher evolutionary rates in invasive species compared to non-invasive species. Additionally, 336 genes in invasive species displayed signs of positive selection, and these genes are involved in physiological processes such as thermogenesis and metabolic rate regulation. Our analysis of the expansion and contraction of orthologous genes and Treefam gene families between invasive and non-invasive species revealed that three orthologous gene families and 18 Treefam gene families were significantly expanded in invasive species. These expanded gene families are primarily associated with material and energy metabolism, as well as environmental adaptation. Furthermore, the number of P450 gene families in the genomes of invasive species was more than that in non-invasive species. This study utilizes comparative genomics analysis methods to elucidate common molecular characteristics in the genomic evolution of invasive insect species like the codling moth. These findings provide important theoretical support for understanding the invasion mechanisms and establishing control strategies of invasive pests. Reference | Related Articles | Metrics | Comments（0）

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Research Progress on Biological Control of Root Rot Disease in Root- and Rhizome-Derived Traditional Chinese Medicines XIAO Ouli, WANG Jiale, CHEN Jieyin, DAI Xiaofeng, KONG Zhiqiang Chinese Journal of Biological Control    2025, 41 (3): 511-519.   DOI: 10.16409/j.cnki.2095-039x.2025.02.037 Abstract （309）      PDF（pc） （976KB）（1065）       Knowledge map    Save With the scarcity of wild medicinal plant resources and the increase in market demand, conventionally cultivated medicinal plants have become the dominant source in the market. However, due to soil degradation, imbalance in the rhizosphere microbial community, and the accumulation of allelopathic and autotoxic substances, root rot has emerged as a major disease in the cultivation of root- and rhizome-derived traditional Chinese medicines (RR-TCMs) in single, intensive, large-scale cultivation. It has a high incidence rate and is difficult to control, severely affecting the yield and quality of traditional Chinese medicines (TCMs). Currently, the application of microbial biocontrol agents in the control of diseases and pests of medicinal plants offers promising prospects for the sustainable development of TCMs. This paper presents a review of the antagonistic microorganisms and biological control mechanisms of root rot disease in RR-TCMs, aiming to provide a reference for the control of root rot. Reference | Related Articles | Metrics | Comments（0）

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Identification of Bacteria strain Sneb2010 and Its Control Effect on Melon Fusarium Wilt WU Ji, ZHU Xiaofeng, WANG Yuanyuan, LIU Xiaoyu, ZHAO Di, YANG Ning, DUAN Yuxi, FAN Haiyan, CHEN Lijie Chinese Journal of Biological Control    2024, 40 (6): 1331-1346.   DOI: 10.16409/j.cnki.2095-039x.2024.02.063 Abstract （397）      PDF（pc） （5504KB）（1064）       Knowledge map    Save Melon Fusarium wilt is a serious soil-borne disease caused by Fusarium oxyporum f. sp. melonis. In this study, the bacteria strain Sneb2010 with significant inhibitory effect on F. oxyporum f. sp. melonis was selected from 688 strains of bacteria by the dual culture technique, and the inhibition rate was 78.45%. The bacterium Sneb2010 was identified as Bacillus velezensis by morphological, physiological and biochemical characteristics combined with 16S rDNA and gyrA sequence analysis. The mycelium growth and spore germination of F. oxyporum f. sp. melonis were significantly inhibited by strain Sneb2010. After Sneb2010 treatment, the mycelia of F. oxyporum f. sp. melonis in the inhibited area showed abnormal phenomena, such as wizened, wrinkled, irregular protrusion and fracture. Strain Sneb2010 could promote the germination of melon seeds and the growth of seedlins. It was found that the strain Sneb2010 could produce protease and had the ability of biofilm forming. In addition, strain Sneb2010 showed strong inhibitory effects on a variety of pathogenic fungi. The results of pot experiment showed that, the control effect of Sneb2010 on melon Fusarium wilt was 64.29%, the number of pathogens decreased by 47%. In conclusion, B. velezensis Sneb2010 could effectively control melon Fusarium wilt, and provide a new potential biocontrol resource for the biological control of melon Fusarium wilt. Reference | Related Articles | Metrics | Comments（0）

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Initial Exploration of Cordyceps javanica and Neoseiulus barkeri Combined Control Technology for Tetranychus urticae ZHAO Yu, MAO Ruixia, XING Peixiang, DIAO Hongliang, LI Xianwei, MA Ruiyan Chinese Journal of Biological Control    2024, 40 (6): 1250-1259.   DOI: 10.16409/j.cnki.2095-039x.2024.11.001 Abstract （258）      PDF（pc） （1625KB）（1060）       Knowledge map    Save To assess the potential applications of the Cordyceps javanica IJ-tg19 combined with Neoseiulus barkeri for the control of the Tetranychus urticae, the virulence of strain IJ-tg19 against T. urticae and the N. barkeri was determined in indoor experiments. Subsequently, greenhouse and field trials were conducted to evaluate the efficacy of the fungus combined with N. barkeri in controlling T. urticae. The results showed that under the treatment of a spore suspension with a concentration of 1×108 spores/mL, the strain IJ-tg19 had a half-lethal time (LT50) of 3.23 days for the adult stage of the T. urticae, and a lethal concentration (LC50) of 1.67×105spores/mL at 5 days after inoculation. Both biological control measures, whether applied individually or in combination, were effective in controlling the T. urticae, but the combined treatment had a higher control efficacy (96.74%, 98.90%). In the field application trial of “C. javanica-N. barkeri” in the control and treatment areas, there was a significant difference in the population density of the T. urticae. The T. urticae was detected 24 days earlier in the control area compared to the treatment area. The peak population densities in the control area and treatment area were 102 mites/leaf (29, November) and 7.3 mites/leaf (5, December) respectively. This study demonstrates that C. javanica IJ-tg19 combined with N. barkeri is more effective than their individual application, which highlights the potential of the application of fungus and N. barkeri for the prevention and biological control of greenhouse T. urticae. Reference | Related Articles | Metrics | Comments（0）

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Analyses of Transcriptome Expression Profiles during Interaction between Trichoderma longibrachiatum and Aspergillus flavus QI Ting, WANG Xinyu, HUANG Shihui, HU Fengbin, XU Tingting, LIANG Caikang, WU Lijuan, NIU Xi, RAN Xueqin, WANG Jiafu Chinese Journal of Biological Control    2025, 41 (2): 321-334.   DOI: 10.16409/j.cnki.2095-039x.2025.02.021 Abstract （199）      PDF（pc） （2907KB）（1049）       Knowledge map    Save To clarify the molecular antagonistic mechanism of Trichoderma inhibiting the growth of Aspergillus flavus, colonies of T. longibrachiatum and A. flavus were collected during their interaction periods, and the enzymes activities to degrade cell wall and antioxidant were detected via ELISA. The differentially expressed genes (DEGs) and the biological processes were further analyzed during the interaction between two fungi using RNA-seq method. The results indicated that the colonies growth rates of T. longibrachiatum were significantly faster with a plate inhibiting rate against A. flavus of 81.1% at the same environment. T. longibrachiatum generated larger amounts of cellulase, chitinase and β-1,3-glucanase. Moreover, the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were suppressed, while the content of malondialdehyde (MDA) was boosted in A. flavus. Compared with control groups, those DEGs were detected to be 2202, 755 and 789 in A. flavus, and 8507, 3055 and 1363 in T. longibrachiatum at early, middle and late stages, respectively. And eleven pivotal interacting candidate genes were screened based on the transcriptomic analysis. These candidate genes are mainly involved in biological processes such as cell wall synthesis, cell membrane permeability, redox reaction, etc. It suggested that T. longibrachiatum could inhibit the effects of antioxidant system and antagonize the growth of A. flavus by up-regulating genes coding for enzymes to degrade cell wall and cell membrane, and by inhibiting the expression of heat shock protein genes in A. flavus. The results would provide a theoretical foundation to take T. longibrachiatum as a biocontrol resource against A. flavus and the production of aflatoxin. Reference | Related Articles | Metrics | Comments（0）