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Comparative Transcriptomic Profiles Revealed JA- and SA- Regulated Soft Rot Disease Resistance by Bacillus velezensis BCP6 in Amorphophallus konjac ZHANG Meng, CHEN Sen, TANG Dengguo, HUANG Yan, LONG Zhijian, WANG Boya, HU Shanglian, CAO Ying Chinese Journal of Biological Control    2024, 40 (6): 1375-1385.   DOI: 10.16409/j.cnki.2095-039x.2024.02.060 Abstract （251）      PDF（pc） （4362KB）（7373）       Knowledge map    Save The application of Bacillus velezensis has been proven to significantly enhance the resistance to soft rot in Amorphophallus konjac, yet its precise biological control mechanisms remain unclear. This study explored the effects of B. velezensis BCP6 on the defense pathways of A. konjac in response to P. aroidearum MY11 infection through comparative transcriptome analysis. Differential expression analysis, gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA) revealed that BCP6 application upregulated pathways related to jasmonic acid (JA), reactive oxygen species, and cell wall synthesis, thereby activating the basic defense system of A. konjac. This immune activation facilitated the rapid initiation of defense responses when subjected to subsequent P. aroidearum infection by enhancing the JA-mediated induced systemic resistance (ISR) and SA-dependent systemic acquired resistance (SAR) signaling pathways. Simultaneously, a significant increase in the expression of genes involved in cell wall formation and pathogenesis-related proteins was observed, effectively enhancing resistance to soft rot. Meanwhile, exogenous JA treatment was found to directly stimulate the expression of cell wall-related genes, namely CESA2 and CSI1, while SA significantly induced the expression of SA-dependent defense genes TLP and E13C. The results provided a theoretical basis for biological control and disease-resistant breeding of konjac. Reference | Related Articles | Metrics | Comments（0）

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Inhibition Effect and Transcriptome Analysis of Isooctyl alcohol on Botrytis cinerea DUAN Tiankun, WANG Yan, YUAN Jiaqi, SU Yaxin, SHI Luxin, WANG Lin, SU Jian, WANG Meiqin, WANG Chunwei Chinese Journal of Biological Control    2023, 39 (6): 1434-1445.   DOI: 10.16409/j.cnki.2095-039x.2023.02.068 Abstract （294）      PDF（pc） （2850KB）（6926）       Knowledge map    Save To reveal the inhibitory mechanism of isooctyl alcohol to Botrytis cinerea, the inhibition effect of isooctyl alcohol on B. cinerea was determined using two-sealed-base-plate method. Mycelial morphology treated with isooctyl alcohol was observed by scanning electron microscopy. The influence of isooctyl alcohol on the extracellular conductivity and the ergosterol synthesis were measured. Response of B. cinerea to isooctyl alcohol was analyzed by RNA sequencing (RNA-Seq). The results showed that the inhibition rate of isooctyl alcohol to mycelia was 67.92%. After treatment with isooctyl alcohol, lots of mycelia were deformed and distorted with the increasing of membrane permeability. At 4, 5 and 6 days after isooctyl alcohol treatment, the ergosterol contents in cell membrane decreased to 44.35%, 38.82% and 42.43%, and the protein concentration decreased to 65.70%, 77.93% and 58.57%, respectively. A total of 577 differentially expressed genes (DEGs), including 312 up-regulated genes and 265 down-regulated genes, were obtained by RNA-Seq. Based on functional analysis of clusters of orthologous groups (COG), gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG), DEGs involved in membrane integral components, ergosterol biosynthesis pathway, amino acid metabolism and ribosome biogenesis pathway were significantly down-regulated, indicating that isooctyl alcohol might affect ergosterol content, energy production and ribosome structure, and further play inhibit role in the growth of B. cinerea. Reference | Related Articles | Metrics | Comments（0）

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Advances in Biological Control of Tuta absoluta (Meyrick) Based on Predatory Natural Enemy Resources LUO Taotao, YAN Shuyan, CAO Mengyu, ZHANG Jinghang, ZHANG Guifen, WAN Fanghao, ZHANG Ya, LI Tao, LIU Shuangqing, ZHANG Yibo Chinese Journal of Biological Control    2024, 40 (4): 727-738.   DOI: 10.16409/j.cnki.2095-039x.2024.02.039 Abstract （565）      PDF（pc） （432KB）（5579）       Knowledge map    Save Tuta absoluta (Meyrick) is a major invasive alien species worldwide, which mainly damages tomato crops by mining leaves and fruits. Since its invasion in Yili, Xinjiang in 2017, it has spread to many provinces (municipalities and autonomous regions) in China, with outbreaks in some places, seriously threatening the healthy production of China's tomato industry. At present, biological control based on predatory natural enemies is one of the main measures for integrated management of T. absoluta. So far, few review papers have been published on the predatory natural enemy resources of tomato leaf miner. Therefore, this paper systematically sorted out the species of predatory natural enemies of T. absoluta worldwide, summarized the predatory ability of predatory natural enemies of tomato leaf miner, the life parameters of predatory natural enemies of T. absoluta, and the effects of chemical pesticides on the prevention and control of T. absoluta by predatory natural enemies. The existing problems and development direction of T. absoluta control by predatory natural enemies were analyzed in order to provide reference for the biological control of T. absoluta in China. Reference | Related Articles | Metrics | Comments（0）

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Bionomics and the Control of the Tea Stick Thrips, Dendrothrips minowai Priesner ZHANG Fengge, CAI Xiaoming, LUO Zongxiu, BIAN Lei, LI Zhaoqun, FU Nanxia, ZHOU Li, CHEN Zongmao, XIU Chunli Chinese Journal of Biological Control    2024, 40 (1): 219-228.   DOI: 10.16409/j.cnki.2095-039x.2024.01.001 Abstract （255）      PDF（pc） （1144KB）（4200）       Knowledge map    Save  The tea stick thrips, Dendrothrips minowai Priesner, is one of the most important sucking pests in tea plantations in China. Both D. minowai adults and nymphs feed on fresh and tender leaves, causing leaves to turn curling, brittle and scorched and finallyfall off, which seriously affect the growth of tea plants and the quality of tea products. In this paper, we reviewed the biological and ecological characteristics of D. minowai such as their morphological characteristics, identification method, geographical distribution, damage symptoms, individual development and life cycle, as well as genetic differentiation. In addition, we also summarized the research progress in the management of D. minowai. At the same time, we suggest that the basic research of the olfactory mechanism and the green prevention and control strategy such as monitoring, mass trapping and the use of pathogenic resources in prevention and control of the pest be accelerated. The review provides a theoretical basis and technical support for the integrated pest management of the thrips. Related Articles | Metrics | Comments（0）

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Research Progress on Green and Sustainable Management Technologies for Spodoptera frugiperda WEI Lei, NIE Ying, LI Yongjun Chinese Journal of Biological Control    2025, 41 (1): 207-219.   DOI: 10.16409/j.cnki.2095-039x.2024.07.004 Abstract （489）      PDF（pc） （530KB）（3868）       Knowledge map    Save The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), is a major worldwide disastrous pest. Since its invasion into China in 2019, the implementation of emergency chemical control measures yielded positive outcomes. However, the occurrence of S. frugiperda in China has now become common and year-round, necessitating a long-term approach for its management. Throughout the history of global pest control, reliance on chemical agents has invariably led to the development of pest resistance. Incidences of pesticide resistance in S. frugiperda have been reported in certain regions of China. Therefore, to mitigate the adverse effects of chemical pesticides and the risk of pesticide resistance, China's management strategies must transition towards green, efficient, precise, and sustainable approaches. On the basis of the regional control strategy and unified management strategy, the Ministry of Agriculture and Rural Affairs has advocated a management framework emphasizing biological control as the focal point, ecological and agricultural control as the foundation, and chemical control as the last resort. This article elaborates on recent technological advancements both domestically and internationally in biological control, ecological and agricultural control methods, with an emphasis on biological control. Additionally, it proposes future research directions and ideas to overcome existing challenges, aiming to provide guidance for the development of green management technologies in China. Reference | Related Articles | Metrics | Comments（0）

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Study on Remediation of Soil Contaminated of Nicosulfuron by Aspergillus terreus DT2 Solid Inoculum ZHANG Jianhua, WANG Xinting, BAI Wenbin, CAO Changlin Chinese Journal of Biological Control    2023, 39 (6): 1463-1473.   DOI: 10.16409/j.cnki.2095-039x.2023.02.056 Abstract （211）      PDF（pc） （485KB）（3479）       Knowledge map    Save In order to solve the problem of nicosulfuron residue in corn field leading to drug injury in the next crop of sorghum, this study prepared DT2 solid bactericide by single factor optimization method, and combined indoor pot experiment with field experiment to add nicosulfuron and apply bactericide DT2 to soil. The repair effect of DT2 solid bactericide on the residual soil of nicosulfuron and its mitigation effect on sorghum were determined. The optimal conditions for solid fermentation of Aspergillus terreus DT2 were pH value 7, inoculation amount 10% and fermentation time 168 h. The effective viable bacteria count can still reach 3.3×108cfu/g after storage at room temperature for 90 days. The results of the indoor pot experiment showed that when the weight of nicosulfuron in soil was 0.023 and 0.046 mg/kg, the inhibition rate of nicosulfuron on sorghum plant height decreased from 26.95% and 49.23% to 1.79% and 2.24% after 30 days of treatment with bacterial agent 6.25 and 12.5 g/kg, respectively. when the weight of nicosulfuron in soil was 0.094 mg/kg, the inhibition rate of nicosulfuron on sorghum plant height was reduced from 51.39% to 4.89% after adding 25 g/kg bactericide for 40 days. When the weight of nicosulfuron in soil was 0.046 mg/kg, adding bacterial agent 12.5 g/kg, 10 days after treatment, the residual rate of nicosulfuron in soil decreased by 56.42% when the weight of nicosulfuron in soil was 0.05 mg/kg, which was significantly different from the control. The mass of nicosulfuron in soil was 0 at 30 days after treatment. DT2 can improve the soil environment, enhance the activity of soil enzymes such as soil urease, alkaline phosphatase, catalase, and soil microbial diversity such as bacteria and fungi. After 40 days of application, nicosulfuron could not only alleviate the inhibition of chlorophyll, net photosynthetic rate and root activity of sorghum, but also promote the growth of sorghum plant height, root length and fresh weight. Field test results showed that the addition of DT2 solid inoculum can effectively reduce the damage of nicosulfuron residue on sorghum, increase the seedling emergence rate and yield, and reduce the disease rate and plant height inhibition rate. When the dosage of nicosulfuron in soil was 20, 30 and 40 g a.i/hm2, adding 300, 450 and 600 kg/hm2 of DT2 solid inoculum, respectively, could reduce the inhibition of nicosulfuron residue on sorghum to the level of no significant toxicity. In conclusion, Aspergillus terreus DT2 bactericide has a good remediation effect on nicosulfuron residues in soil and has potential application prospects. Reference | Related Articles | Metrics | Comments（0）

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Screening and Identification of Biocontrol Potential Myxobacteria Strains against Fire Blight and Pear Canker Diseases and Evaluation of Indoor Control Efficacy BAI Xueying, HAN Jian, SUN Boyuan, SONG Shumin, LUO Ming, SUN Liying Chinese Journal of Biological Control    2023, 39 (6): 1384-1397.   DOI: 10.16409/j.cnki.2095-039x.2023.02.059 Abstract （279）      PDF（pc） （3040KB）（3470）       Knowledge map    Save Fire blight causted by Erwinia amylovora and pear canker causted b Valsa pyri are two major diseases affecting the production of Pyrus sinkiangensis. This study aimed to develop biological control agent for combating fire blight and pear canker diseases by utilizing myxobacteria strains isolated from soil samples collected from different prefectures in Xinjiang. The predation efficiency of myxobacteria strains on E. amylovora was compared by using bacterial lawn co-culture, while the inhibitory effect of myxobacteria strains on hyphal growth and conidial germination of V. pyri was assessed by the pair-culuturing method. Myxobacteria strains with significant predation efficacy and antagonistic activities against E. amylovora and V. pyri were screened, and the control effects of the strains on these two diseases were further evaluted by detached leaves, branches and greenhouse potted experiments. The myxobacteria strains with biocontrol potential were identified based on morphological characteristics, 16S rRNA and lep A gene sequence homology analysis. The results showed that the six selected myxobacteria strains exhibited substantial predation effects on E. amylovora, demonstrated by significant reduction of the bacterial numbers from 109 to 102～105 cfu/mL. Additionally, these strains demonstrated strong antagonistic activity against V. pyri, with inhibition rates of mycelial growth and conidial germination over 70% and 87%, respectively. Strains NST12 and NST47 showed protective and therapeutic effects of over 75% against fire blight on detached P. sinkiangensis leaves and P. betulifolia seedlings, while strains NST47 and NST49 exhibited protective and therapeutic effects of over 72% and 54% against pear canker on detached twigs, respectively. Notably, strain NST47 displayed remarkable biocontrol potential against both fire blight and pear canker. Based on the morphological and molecular identification results, strains NST49 and NST12 were identjfied as Myxococcus fulvus and NST47 was identjfied as Archangium violaceum. Reference | Related Articles | Metrics | Comments（0）

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Isolation and Identification of Endophytic Bacteria from Walnut Leaves and Their Biocontrol Potential against Walnut Anthracnose GU Yuxin, WANG Shuhe, WANG Shenghao, LIU Shengming, KANG Yebin Chinese Journal of Biological Control    2024, 40 (5): 1099-1112.   DOI: 10.16409/j.cnki.2095-039x.2024.02.058 Abstract （319）      PDF（pc） （4367KB）（3304）       Knowledge map    Save Walnut anthracnose, caused by several Colletotrichum genus species, is recognized as one of the most destructive and economically important diseases, resulting in considerable losses. The implementation of biological control is expected to provide a favorable alternative to chemical methods for disease management, as it is environmentally friendly, less prone to resistance development, and offers high safety levels. In the present study, an endophytic bacteria strain GYX7, isolated from healthy walnut leaves, exhibited the most potent inhibitory activity against the pathogens of walnut anthracnose, including C. gloeosporioides, C. fructicola, C. siamense, C. fioriniae and C. aenigma, with inhibition rates ranging from 70.56% to 80.78%. Microscopic examination indicated that strain GYX7 induced morphological distortions and lysis of the mycelium during confrontation culture. Furthermore, strain GYX7 demonstrated a broad-spectrum antifungal, inhibiting 13 phytopathogens except Pythium aphanidermatum. Based on morphological, physiological, and biochemical characteristics and a phylogenetic tree constructed using 16S rDNA and gyrB gene sequences, strain GYX7 was identified as Bacillus velezensis. The volatile compounds produced by strain GYX7 were found to contribute to its biocontrol capabilities. The aseptic filtrate of strain GYX7 at a 20% concentration significantly inhibited the growth and spore germination of C. fructicola, with inhibition rates of 82.24% and 98.17%, respectively. Enzyme activity assays indicated that strain GYX7 produced various hydrolytic enzymes, including protease, cellulase, pectinase, and β-1,3-glucanase. Additionally, the strain was found to possess genes that encode antimicrobial substances, including surfactin, bacillomycin, fengycin, iturin, bacillaene, bacillibactin, bacilysin, macrolactin and difficidin, as identified through polymerase chain reaction (PCR). In vitro inoculation experiments showed that strain GYX7 effectively reduced the incidence and severity of walnut anthracnose. These findings suggest that strain GYX7 has the potential to be a biocontrol agent for managing walnut anthracnose. Reference | Related Articles | Metrics | Comments（0）

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Regulation of Longevity and Antioxidant Activity in Entomopathogenic Nematodes Steinernema kraussei by Chlorogenic Acid CHEN Hong, LI Xitong, WANG Xingduo, QIAN Xiujuan Chinese Journal of Biological Control    2024, 40 (1): 108-116.   DOI: 10.16409/j.cnki.2095-039x.2023.01.040 Abstract （195）      PDF（pc） （879KB）（3280）       Knowledge map    Save The effects of chlorogenic acid (CGA) on the longevity and antioxidant activity of the entomopathogenic nematodes (EPNs) Steinernema kraussei were investigated through treating S. kraussei with different concentrations of chlorogenic acid (0, 50, 100, 500, 1000 µmol/L) and then recording S. kraussei longevity, antioxidant activity, lipofuscin levels, and related genes mRNA expression levels, with the aim to explore the potential mechanisms of action of chlorogenic acid. The results showed that the maximum longevity of S. kraussei was increased by 36.36% in the treatment with100 µmol/L chlorogenic acid in comparison to the control, significantly inhibited the accumulation of lipofuscin related to aging, but increased SOD and CAT enzyme activities by 1.97 and 2.47 times, respectively. Moreover, qRT-PCR results showed that daf-16 and gst-4 genes transcription levels was increased by 1.78 and 3.51, respectively, in the nematodes fed with 100 µmol/L CGA compared with the control. However, the daf-2 gene transcription level showed a downward trend and decreased by 0.13. This study demonstrates that chlorogenic acid enhances the antioxidant activity and prolong the longevity of entomopathogenic nematodes S. kraussei by regulating the insulin/IGF-1 signaling pathway. Related Articles | Metrics | Comments（0）

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Bioactivity of Four Essential Oils of Artemisia Plants against Drosophila suzukii Matsumura LING Lijun, WANG Yuanyuan, JIANG Kunling, CHENG Wenting, FENG Shenglai, LU Lu, CHEN Xuelin Chinese Journal of Biological Control    2023, 39 (6): 1341-1350.   DOI: 10.16409/j.cnki.2095-039x.2023.01.034 Abstract （261）      PDF（pc） （449KB）（3071）       Knowledge map    Save Drosophila suzukii Matsumura is a serious threat to fruit production around the world due to its fast spread and wide distribution. Essential oils of four Artemisia plants including A. giraldii, A. scoparia, A. anethoides, and A. annua were evaluated for their biological activities against D. suzukii, aiming to exploit new environmentally friendly plant-derived insecticides. The insecticidal activity of the four essential oils against D. suzukii was determined by fumigation test, repellency test and larval mortality test; the chemical composition of the four essential oils was analyzed using gas chromatography-mass spectrometer (GC-MS). The fumigation test showed that the four essential oils all had good insecticidal activities against D. suzukii, and the fumigation activities of A. giraldii essential oil was the best, with LC50 value of 1.421 μL/mL, followed by A. anethoides essential oil, with LC50 value of 3.499 μL/mL, and A. annua essential oil came at the last. The repellent test showed that A. giraldii essential oil was of the highest repellent activities, with repellency indices of 86%, and the A. annua oil was the worst, with repellency indices of 50%. The larval mortality test showed that A. scoparia essential oil resulted in 70% mortality of D. suzukii larvae, while A. anethoides essential oil was the least effective, with only 40% larval mortality. GC-MS analysis showed that there were 82 components in the four essential oils, and β-Caryophyllene, Terpinen-4-ol, and 1,8-Cineole are common compounds in the four essential oils. The above results indicate that the four Artemisia essential oils have the potential to be developed as an insecticide for D. suzukii. This research paves a theoretical foundation for the development of green pollution-free plant-derived pesticides. Reference | Related Articles | Metrics | Comments（0）

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The Nematicidal Activity of Clonostachys pseudochoroleuca Clpse01 on Botrytis cinerea XU Yong, PENG Zijia, XU Zhengmei, SHAO Yakun, LAN Yan, XU Qinghua, YU Zhongdong Chinese Journal of Biological Control    2024, 40 (1): 186-195.   DOI: 10.16409/j.cnki.2095-039x.2023.02.066 Abstract （232）      PDF（pc） （5048KB）（3003）       Knowledge map    Save A parasitic fungus with nematocidal activity, Clpse01, was found while pine wood nematodes (Bursaphelenchus xylophilus) were feeding on Botrytis cinerea media. Clpse01 morphologies and parasite evidences were seen under the microscope. Multiple genes, including ITS, large subunit ribosomal DNA, β-tublin and elongation factor, were used for further limiting by the phylogenic tree. Clpse01's nematocidal effectiveness against pine wood nematodes, as well as its effects on egg hatching and reproduction, were investigated. The Clpse01 fermentation fluid was analyzed for extracellular protein concentrations, β-glucosidase activity, and chitinase activity. Clpse01 was identified as Clonostachys pseudochloleuca using a combination of morphological and molecular phylogenetic approaches. C. pseudochroleuca Clpse01 was discovered entangling and infiltrating B. cinerea hyphae, acquiring nutrients from them while limiting B. cinerea normal growth. Inoculating 2000 pine wood nematodes into media with full growth of isolate Clpse01 resulted in only 6±4 live ones after 8 days, while nematodes isolated from the control medium of B. cinerea reached 33066±6882. The adjusted mortality rate of pine wood nematode treated with C. pseudochroleuca Clpse01 fermentation broth for 48 h was 88.0%, while that treated with 1╳108 conidiospore suspensions per milliliter for 24 h was 88.33%. In double diluent fermentation broth for 24 hours, egg hatching was completely inhibited. After 6 hours of fermentation, the Clpse01 liquid contained 1782.917 μg/mL of total extracellular protein, 9.99 U/mL of β-glucosidase, and 0.8792 U/mL of chitinase. Protein concentrations increased with time. In general, C. pseudochroleuca Clpse01 functions by parasitizing B. cinerea, the feeding fungus, and influencing B. xylophilus reproduction, survival, and egg hatching. Related Articles | Metrics | Comments（0）

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Research Progress and Prospect of DNA Barcoding in Parasitoid Wasps SUN Xingxing, JIANG Yingjie, WANG Fan, ZHANG Lisheng Chinese Journal of Biological Control    2025, 41 (2): 473-491.   DOI: 10.16409/j.cnki.2095-039x.2024.07.007 Abstract （205）      PDF（pc） （530KB）（2885）       Knowledge map    Save Parasitoid wasps play a crucial role in the biological control of agricultural and forestry pests, such as lepidoptera and hemiptera. They have significant potential for development and application in biological control strategies. DNA barcoding, a modern molecular technology widely used in animal and plant biology, ecology, and other fields, has emerged as an important tool for classifying parasitic wasps and conducting ecological research. This paper reviews relevant literature to summarize the primary DNA barcodes and primers utilized for parasitoid wasps. It also discusses the applications of DNA barcoding in taxonomic identification, detection of new or cryptic species, analysis of food web relationships, and phylogenetic research on parasitic wasps. Furthermore, this paper analyzes current challenges faced by DNA barcoding research on parasitic wasps while proposing prospects for key issues such as selecting appropriate DNA barcodes, screening suitable phylogenetic models, and utilizing high-throughput sequencing technology. The aim is to provide theoretical references for resource exploration of parasitic wasps, optimization of biological control strategies, and advancements in evolutionary biology. Reference | Related Articles | Metrics | Comments（0）

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First Report of a Parasitic Wasp Streblocera okadai Parasitizing Monolepta signata HE Can, WANG Zhenying, ZHANG Yongjun, ZHANG Tiantao Chinese Journal of Biological Control    2024, 40 (1): 229-234.   DOI: 10.16409/j.cnki.2095-039x.2022.11.023 Abstract （244）      PDF（pc） （1365KB）（2797）       Knowledge map    Save In recent years, Monolepta signata has increased in population size year after year, becoming an essential pest on corn, soybeans, and other crops due to its wide distribution, diverse hosts, and severe damage. From July to September in 2022, a parasitic wasp parasitizing M. signata adults in the fields of Gongzhuling, Jilin province was collected. It was identified by morphology and molecular method as Streblocera (Eutanycerus) okadai Watanabe of Braconidae. Based on CO I genes, the wasp species was phylogenetically clustered with the genus Streblocera. This is the first report of parasitic enemies of M. signata, which may provide a new strategy for the biological control of this pest. Related Articles | Metrics | Comments（0）

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Effects of Feeding Four Host Plants on Midgut Bacteria of Tuta absoluta YANG Yaxian, ZHOU Zhaoxu, QIAN Xiujuan, LIU Yueying, ZHANG Meijiao, JIANG Mingjun Chinese Journal of Biological Control    2024, 40 (4): 776-786.   DOI: 10.16409/j.cnki.2095-039x.2024.02.044 Abstract （206）      PDF（pc） （4081KB）（2738）       Knowledge map    Save Tomato leaf miner (Tuta absoluta) is a worldwide pest with a wide range of host species, causing serious harm to its host crops. The mid-gut bacterial diversity and function of its larvae feeding on tomato, potato, eggplant and ginseng, were analyzed by 16S rRNA sequencing. The results showed that 4886, 6016, 2781, and 3471 operational taxonomic units (OTU, respectively) were detected, among which 905 OTUs were common. Alpha and Beta diversity analyses showed mid gut bacterial diversities were the highest in the larvae feeding on potato and eggplant. Species classification analysis showed that, for each host plant, and at each level of classification, mid-gut bacterial species abundance differed. Firmicutes and Proteobacteria were the dominant mid-gut bacterial phyla, Wolbachia was the dominant genus on tomato and potato hosts, and Enterococcus was the dominant genus on ginseng fruit and eggplant hosts. Analyses showed that their metabolic pathways were statistically similar, with the greatest proportion of metabolic pathways for biosynthesis and degradation. In conclusion, though host plant species significantly affect the mid-gut bacterial diversity and community structure of tomato leaf miner larvae, the microbial functions are similar. Reference | Related Articles | Metrics | Comments（0）

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Antifungal and Field-Control Effects of Eugenol and Carvacrol on Ustilaginoidea virens HU Zheng, FAN Kai, YU Yinan, YOU Jiaqi, WANG Xinyi, GUO Dakai, HUANG Qingwen, MENG Jiajia, GUO Wenbo, NIE Dongxia, HAN Zheng Chinese Journal of Biological Control    2023, 39 (6): 1446-1454.   DOI: 10.16409/j.cnki.2095-039x.2023.02.061 Abstract （236）      PDF（pc） （2652KB）（2473）       Knowledge map    Save To develop new fungicides based on natural components for the prevention and control of rice false smut, eugenol and carvacrol, two natural plant-derived terpenoids with rich bioactivities and wide sources, were investigated in this study. Concentration gradient tests showed that both of these two compounds could significantly inhibit the mycelial growth and spore germination of Ustilaginoidea virens in a concentration- dependent manner. At a concentration of 0.2 mmol/L, the inhibition rates of eugenol and carvacrol on mycelial growth were 12.53% and 44.39%, respectively, and the inhibition rates on spore germination were 94.02% and 72.24%, respectively. The scanning electron microscopy confirmed that both eugenol and carvacrol caused disruptions in the integrity of U. virens hyphae. The field efficacy experiments were further performed and the results showed that both of these two natural products were effective in inhibiting the incidence of rice false smut and reducing the accumulation of ustiloxin A in paddy. In conclusion, eugenol and carvacrol were successfully applied to control rice false smut and prevent the production of ustiloxin A in this study, and the underlying mechanisms were preliminarily analyzed, providing their broad application prospects in the future. Reference | Related Articles | Metrics | Comments（0）

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Investigation and Molecular Evaluation of the Natural Enemies of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in Tomato Fields in Yunnan Province ZHANG Guifen, ZHU Huangkang, HUANG Liang, WANG Yusheng, LI Tao, HUANG Cong, XIAN Xiaoqing, XUE Yantao, GUI Furong, LIU Wanxue, WAN Fanghao, ZHANG Yibo Chinese Journal of Biological Control    2024, 40 (4): 739-749.   DOI: 10.16409/j.cnki.2095-039x.2023.11.004 Abstract （306）      PDF（pc） （421KB）（2414）       Knowledge map    Save Yunnan Province is one of the two earliest invaded areas of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in China. The potential indigenous natural enemies of T. absoluta were investigated, collected, and identified during July to October in tomato planting in 10 prefectures of Yunnan Province. The species of collected natural enemies were identified using DNA barcoding technique and their control efficacy on T. absoluta in tomato fields were evaluated using T. absoluta species-specific PCR (SS-PCR) technique. Our results indicated that there were 36 predatory species detected in tomato fields, belonging to 16 families, 5 orders, 2 classes. The results of SS-PCR detection shown that 19 species, belonging to 12 families, 3 orders, 2 classes, fed on T. absoluta. Among the 19 predatory natural enemy species, 8 species belong to Insecta and 11 species belong to Arachnida. The percentages of positive detection of T. absoluta were between 5.7% and 100.0%. Of the 19 predatory species, the most widely distributed was Nesidiocoris tenuis Reuter (Hemiptera: Miridae), which was detected in 8 prefectures, followed by Oirus agilis (Flor), Orius sp. (Hemiptera: Anthocoridae), and Coccinella septempunctata L. (Coleoptera: Coccinellidae), which were detected in 5, 4, and 5 prefectures, respectively. Pardosa pseudoannulata (Böesenberg et Strand) (Araneae: Lycosidae) were detected in 3 prefectures and the other 14 predatory species were detected only in one or two prefectures. Of the 10 tomato planting prefectures, Zhaotong had the most abundant natural enemy species of T. absoluta, where 8 predatory species were identified, followed by Lijiang and Yuxi, where 6 and 5 predatory natural enemy species were identified, respectively. Four and three predatory natural enemy species were identified in Kunming and Qujing, respectively, two species identified in Dali, Wenshan, and Honghe, and only one species was identified in Chuxiong and Puer. Comprehensive evaluation, based on percentage of positive detection of T. absoluta, dominance of predatory natural enemy population, and distribution frequency, showed that N. tenuis had the greatest potential for control of T. absoluta, followed by O. agilis, C. septempunctata and P. pseudoannulata also had good potential for control of T. absoluta. Nowadays, the occurrence of T. absoluta in tomato fields in some rejoins of China is serious, however, the rich species of natural enemies in Southwestern China can provide reliable natural resource for utilizing domestic natural enemies to manage T. absoluta. Reference | Related Articles | Metrics | Comments（0）

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Isolation and Identification of Arthrobacter pascens S17 and Its Ability to  Promote Growth of Cucumber ZENG Xianfeng, SUN Yuchen, XIE Xuewen, SHI Yanxia, CHAI Ali, LI Baoju, LI Lei Chinese Journal of Biological Control    2024, 40 (1): 178-185.   DOI: 10.16409/j.cnki.2095-039x.2023.02.070 Abstract （210）      PDF（pc） （1772KB）（2394）       Knowledge map    Save  In order to screen out the biocontrol bacteria with growth-promoting effect, soil samples were collected from Hebei, Shandong and other places, and the growth-promoting strains with high-yield IAA ability were isolated and screened, and the potential growth-promoting strains with growth-promoting ability were identified based on morphological characteristics, physiological and biochemical indexes and multi-gene phylogenetic tree analysis, and the growth-promoting effects of growth-promoting strains on cucumber seed germination and cucumber seedlings in pot experiments and field experiments were determined. The results showed that one bacterium, Arthrobacter pascens S17, which had a significant growth-promoting effect on cucumber seedlings, was isolated and identified. Arthrobacter pascens S17 could synthesize IAA in large quantities in DF+ medium, and the highest IAA content was 128.54 μg/mL. The growth rate of cucumber seedling plant height and leaf area of cucumber seedlings reached 35.99% and 26.90% by root irrigation treatment with Arthrobacter pascens S17, and the physiological indexes of cucumber seedlings were significantly higher than those of the control. Arthrobacter pascens S17 has a significant effect on cucumber growth, and has a certain development and application prospect. Related Articles | Metrics | Comments（0）

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Biological Function of Juvenile Hormone Binding Protein MsJHBP3 and its Effects on the Growth and Development of Mythimna separata HU Yushuo, ZHANG Chunyu, MENG Xiangxin, LI Shuohan, ZHANG Jinbo, FAN Dong Chinese Journal of Biological Control    2024, 40 (1): 80-90.   DOI: 10.16409/j.cnki.2095-039x.2023.01.018 Abstract （212）      PDF（pc） （513KB）（2389）       Knowledge map    Save Juvenile hormone binding protein (JHBP), a carrier protein binds with juvenile hormone (JH) to form a complex for the transport of juvenile hormone to its target organs and the regulation of growth and development of insects. In this study, 7 juvenile hormone binding protein cDNA sequences were screened in the transcriptome of M. separata, and a highly expressed juvenile hormone binding protein MsJHBP3 (GenBank accession number: MZ577068) when induced by juvenile hormone was obtained. The full length of MsJHBP3 cDNA sequence was 839 bp with an open reading frame (ORF) of 729 bp, encoding 242 amino acids. The isoelectric point and predicted molecular weight were 5.35 and 27.02 kDa, respectively. In different developmental stages, MsJHBP3 was expressed the highest in the pupal stage and the lowest in the second instar larval stage. When the 4th instar larvae were treated with siRNA interference for 6 h, the gene silencing efficiency reached 79.72%, the content of juvenile hormone increased by 1.14 folds, while the pupation rate and the emergence rate significantly decreased by 7.42% and 3.75%, respectively, compared to the control. Transcriptome data analysis showed 212 differentially expressed genes, including 105 up-regulated and 107 down-regulated genes. Among them, juvenile hormone methyltransferase gene, juvenile hormone epoxide hydrolase gene, and juvenile hormone esterase gene were up-regulated. MsJHBP3 was involved in the growth and development and the regulation of JH metabolism. These results provide basic data for the excavation of key genes in the growth and development of M. separata, providing new target gene and pathway for M. separata control. Related Articles | Metrics | Comments（0）

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Isolation,Identification and Evaluation of Insecticidal Activity of an Entomopathogenic Bacteria Strain Infecting Post-overwintering Ostrinia furnacalis Larvae YUAN Zihan, WANG Xiaowu, DING Xinhua, FU Kaiyun, JIANG Xudong, GONG Xuehua, JIA Zunzun, TOULXUN·Ahemaiti, GUO Wenchao Chinese Journal of Biological Control    2024, 40 (2): 310-318.   DOI: 10.16409/j.cnki.2095-039x.2024.01.002 Abstract （283）      PDF（pc） （504KB）（2361）       Knowledge map    Save A bacterial strain ACB3014 was isolated from the infected overwintering larvae of Ostrinia furnacalis in spring sown corn fields in Xinyuan County,Yili Valley,Xinjiang and identified by morphological observation,physiological and biochemical characteristics and 16S rDNA sequence analysis.The pathogenicity of this strain against the 3rd instar larvae of O.furnacalis was determined by feeding method.The strain ACB3014 was Serratia marcescens.The cumulative corrected mortality of ACB3014 on the 3rd instar larvae increased with the increase of bacterial suspension concentration.On the 7th day after feeding ACB3014 bacterial suspension,the cumulative adjusted mortality of the 3rd instar larvae ranged between 27.78%—77.78% at the concentration of 2× 104—2× 108 cfu/mL.On the 13th day of inoculation,when the concentration reached 2× 108 cfu/mL,LC50 and LT50 against the 3rd instar larvae of O.furnacalis were 3.79× 103 cfu/mL and 3.93 days,respectively.In addition,the infected O.furnacalis larvae are dark red and black and are pyoid and festering,giving off a strong odor.In conclusion,ACB3014 strain has good insecticidal activity against O.furnacalis larvae and has a high potential as a green biological control resource for O.furnacalis. Reference | Related Articles | Metrics | Comments（0）

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Isolation, Identification and Control Effect of Strain ZF510 on Bacterial Soft Rot of Chinese Cabbage QIN Shiyang, LU Lingchen, XIE Xuewen, SHI Yanxia, CHAI Ali, WANG Yuanhong, LI Baoju, LI Lei Chinese Journal of Biological Control    2024, 40 (1): 146-156.   DOI: 10.16409/j.cnki.2095-039x.2024.02.003 Abstract （298）      PDF（pc） （2471KB）（2311）       Knowledge map    Save Soft rot of Chinese cabbage is a serious bacterial disease caused by Pectobacterium brasiliense. Currently,,there are few effective products for the control of bacterial soft rot in vegetables. In order to screen biocontrol bacteria with good antagonistic effect against Chinese cabbage bacterial soft rot, this study isolated a strain of beneficial bacteria ZF510 from the rhizosphere soil of cabbage. Through morphological observation, physiological and biochemical characteristics, and multi-gene phylogenetic tree analysis, as well as pot experiments to verify the control effect of the antagonistic strain on Chinese cabbage bacterial soft rot. The results showed that the strain ZF510 was Pseudomonas donghuensis, which produced protease during the metabolic process, and had the ability to synthesize IAA, iron-loving and phosphorus-dissolving ability. Through the analysis of bacteriostatic spectrum, it was proved that strain ZF510 had significant antagonistic effects on seven pathogenic bacteria such as Pectobacterium brasiliensis and Xanthomonas campestris pv. campestris. The dichotomous dish method was used to determine that the volatile substances had inhibitory effects on the three pathogenic fungi of Rhizoctonia solani, Didymella bryoniae and Stemphylium solanacearum. The results of pot experiment showed that the symptoms of bacterial soft rot of Chinese cabbage inoculated with strain ZF510 were significantly reduced, and the control effect reached 44.56%. In summary, strain ZF510 has great potential for biological control and application prospects. Related Articles | Metrics | Comments（0）