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Comparative Transcriptomic Profiles Revealed JA- and SA- Regulated Soft Rot Disease Resistance by Bacillus velezensis BCP6 in Amorphophallus konjac ZHANG Meng, CHEN Sen, TANG Dengguo, HUANG Yan, LONG Zhijian, WANG Boya, HU Shanglian, CAO Ying Chinese Journal of Biological Control    2024, 40 (6): 1375-1385.   DOI: 10.16409/j.cnki.2095-039x.2024.02.060 Abstract （486）      PDF（pc） （4362KB）（10868）       Knowledge map    Save The application of Bacillus velezensis has been proven to significantly enhance the resistance to soft rot in Amorphophallus konjac, yet its precise biological control mechanisms remain unclear. This study explored the effects of B. velezensis BCP6 on the defense pathways of A. konjac in response to P. aroidearum MY11 infection through comparative transcriptome analysis. Differential expression analysis, gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA) revealed that BCP6 application upregulated pathways related to jasmonic acid (JA), reactive oxygen species, and cell wall synthesis, thereby activating the basic defense system of A. konjac. This immune activation facilitated the rapid initiation of defense responses when subjected to subsequent P. aroidearum infection by enhancing the JA-mediated induced systemic resistance (ISR) and SA-dependent systemic acquired resistance (SAR) signaling pathways. Simultaneously, a significant increase in the expression of genes involved in cell wall formation and pathogenesis-related proteins was observed, effectively enhancing resistance to soft rot. Meanwhile, exogenous JA treatment was found to directly stimulate the expression of cell wall-related genes, namely CESA2 and CSI1, while SA significantly induced the expression of SA-dependent defense genes TLP and E13C. The results provided a theoretical basis for biological control and disease-resistant breeding of konjac. Reference | Related Articles | Metrics | Comments（0）

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Research Progress on Green and Sustainable Management Technologies for Spodoptera frugiperda WEI Lei, NIE Ying, LI Yongjun Chinese Journal of Biological Control    2025, 41 (1): 207-219.   DOI: 10.16409/j.cnki.2095-039x.2024.07.004 Abstract （1027）      PDF（pc） （530KB）（7924）       Knowledge map    Save The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), is a major worldwide disastrous pest. Since its invasion into China in 2019, the implementation of emergency chemical control measures yielded positive outcomes. However, the occurrence of S. frugiperda in China has now become common and year-round, necessitating a long-term approach for its management. Throughout the history of global pest control, reliance on chemical agents has invariably led to the development of pest resistance. Incidences of pesticide resistance in S. frugiperda have been reported in certain regions of China. Therefore, to mitigate the adverse effects of chemical pesticides and the risk of pesticide resistance, China's management strategies must transition towards green, efficient, precise, and sustainable approaches. On the basis of the regional control strategy and unified management strategy, the Ministry of Agriculture and Rural Affairs has advocated a management framework emphasizing biological control as the focal point, ecological and agricultural control as the foundation, and chemical control as the last resort. This article elaborates on recent technological advancements both domestically and internationally in biological control, ecological and agricultural control methods, with an emphasis on biological control. Additionally, it proposes future research directions and ideas to overcome existing challenges, aiming to provide guidance for the development of green management technologies in China. Reference | Related Articles | Metrics | Comments（0）

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Research Progress on Damage Characteristics and Parasitoids of Important Pests in Peach SUN Xingxing, WANG Fan, JIANG Yingjie, ZHANG Lisheng Chinese Journal of Biological Control    2025, 41 (1): 231-250.   DOI: 10.16409/j.cnki.2095-039x.2024.03.018 Abstract （393）      PDF（pc） （668KB）（6879）       Knowledge map    Save Prunus persica is one of the vital fruit resources in China, with their growth, development, and fruit yield significantly affected by a wide range of pests. Parasitoid wasps play a crucial role as key biological control agents in effectively managing pests such as peach aphids, peach borer moths, and peach fruit borers. This article provides a comprehensive review of published literature, focusing on the important pests of peach trees and their damage characteristics, the progress in research on related parasitoid wasp resources, and the application of these wasps in integrated pest management. The review elaborated on 21 pests, including mites, that have a significant economic impact on peach trees, and listed 194 species of parasitoid wasps across 20 families that play a pivotal role in the biological control of peach tree pests. Additionally, the article summarized current issues in research on parasitoid wasps for peach tree pests and proposed future research directions, aiming to offer deeper theoretical support for improving pest management strategies and protecting biodiversity in peach orchards. Reference | Related Articles | Metrics | Comments（0）

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Research Progress on Biological Control of Root Rot Disease in Root- and Rhizome-Derived Traditional Chinese Medicines XIAO Ouli, WANG Jiale, CHEN Jieyin, DAI Xiaofeng, KONG Zhiqiang Chinese Journal of Biological Control    2025, 41 (3): 511-519.   DOI: 10.16409/j.cnki.2095-039x.2025.02.037 Abstract （773）      PDF（pc） （976KB）（6341）       Knowledge map    Save With the scarcity of wild medicinal plant resources and the increase in market demand, conventionally cultivated medicinal plants have become the dominant source in the market. However, due to soil degradation, imbalance in the rhizosphere microbial community, and the accumulation of allelopathic and autotoxic substances, root rot has emerged as a major disease in the cultivation of root- and rhizome-derived traditional Chinese medicines (RR-TCMs) in single, intensive, large-scale cultivation. It has a high incidence rate and is difficult to control, severely affecting the yield and quality of traditional Chinese medicines (TCMs). Currently, the application of microbial biocontrol agents in the control of diseases and pests of medicinal plants offers promising prospects for the sustainable development of TCMs. This paper presents a review of the antagonistic microorganisms and biological control mechanisms of root rot disease in RR-TCMs, aiming to provide a reference for the control of root rot. Reference | Related Articles | Metrics | Comments（0）

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Research Progress and Prospect of DNA Barcoding in Parasitoid Wasps SUN Xingxing, JIANG Yingjie, WANG Fan, ZHANG Lisheng Chinese Journal of Biological Control    2025, 41 (2): 473-491.   DOI: 10.16409/j.cnki.2095-039x.2024.07.007 Abstract （405）      PDF（pc） （530KB）（5673）       Knowledge map    Save Parasitoid wasps play a crucial role in the biological control of agricultural and forestry pests, such as lepidoptera and hemiptera. They have significant potential for development and application in biological control strategies. DNA barcoding, a modern molecular technology widely used in animal and plant biology, ecology, and other fields, has emerged as an important tool for classifying parasitic wasps and conducting ecological research. This paper reviews relevant literature to summarize the primary DNA barcodes and primers utilized for parasitoid wasps. It also discusses the applications of DNA barcoding in taxonomic identification, detection of new or cryptic species, analysis of food web relationships, and phylogenetic research on parasitic wasps. Furthermore, this paper analyzes current challenges faced by DNA barcoding research on parasitic wasps while proposing prospects for key issues such as selecting appropriate DNA barcodes, screening suitable phylogenetic models, and utilizing high-throughput sequencing technology. The aim is to provide theoretical references for resource exploration of parasitic wasps, optimization of biological control strategies, and advancements in evolutionary biology. Reference | Related Articles | Metrics | Comments（0）

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Advance in Push-Pull Strategy for Control of the Asian Citrus Psyllid Diaphorina citri LIN Wenhan, WANG Jing, ZHOU Yutong, SANG Wen, QIU Baoli Chinese Journal of Biological Control    2025, 41 (1): 220-230.   DOI: 10.16409/j.cnki.2095-039x.2024.07.002 Abstract （523）      PDF（pc） （488KB）（4727）       Knowledge map    Save Citrus is one of the important economic crops in the world. With the expansion of production scale, pest control has become a bottleneck restricting its development. Diaphorina citri is the most serious worldwide pest among numerous insects that harm citrus. Its adults and nymphs can not only directly stab and damage rutaceae plants such as citrus and lemon, but also transmit citrus Huanglongbing (HLB) as the only natural vector. The ‘Push-pull strategy’, with the advantages of economy, convenience, high-efficiency and green, is crucial in the ecological regulation and management of pests. Popularizing the ‘push-pull’ strategy could contribute to controlling the citrus psyllid and promoting the green development of the citrus industry. This review summarized the current progress from components of push and pull. It is expected that the development trends of push-pull strategy will provide theoretical reference for the economical, convenient and effective control of citrus psyllid and accelerate the healthy and sustainable development of citrus industry. Reference | Related Articles | Metrics | Comments（0）

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Isolation and Identification of Endophytic Bacteria from Walnut Leaves and Their Biocontrol Potential against Walnut Anthracnose GU Yuxin, WANG Shuhe, WANG Shenghao, LIU Shengming, KANG Yebin Chinese Journal of Biological Control    2024, 40 (5): 1099-1112.   DOI: 10.16409/j.cnki.2095-039x.2024.02.058 Abstract （533）      PDF（pc） （4367KB）（4606）       Knowledge map    Save Walnut anthracnose, caused by several Colletotrichum genus species, is recognized as one of the most destructive and economically important diseases, resulting in considerable losses. The implementation of biological control is expected to provide a favorable alternative to chemical methods for disease management, as it is environmentally friendly, less prone to resistance development, and offers high safety levels. In the present study, an endophytic bacteria strain GYX7, isolated from healthy walnut leaves, exhibited the most potent inhibitory activity against the pathogens of walnut anthracnose, including C. gloeosporioides, C. fructicola, C. siamense, C. fioriniae and C. aenigma, with inhibition rates ranging from 70.56% to 80.78%. Microscopic examination indicated that strain GYX7 induced morphological distortions and lysis of the mycelium during confrontation culture. Furthermore, strain GYX7 demonstrated a broad-spectrum antifungal, inhibiting 13 phytopathogens except Pythium aphanidermatum. Based on morphological, physiological, and biochemical characteristics and a phylogenetic tree constructed using 16S rDNA and gyrB gene sequences, strain GYX7 was identified as Bacillus velezensis. The volatile compounds produced by strain GYX7 were found to contribute to its biocontrol capabilities. The aseptic filtrate of strain GYX7 at a 20% concentration significantly inhibited the growth and spore germination of C. fructicola, with inhibition rates of 82.24% and 98.17%, respectively. Enzyme activity assays indicated that strain GYX7 produced various hydrolytic enzymes, including protease, cellulase, pectinase, and β-1,3-glucanase. Additionally, the strain was found to possess genes that encode antimicrobial substances, including surfactin, bacillomycin, fengycin, iturin, bacillaene, bacillibactin, bacilysin, macrolactin and difficidin, as identified through polymerase chain reaction (PCR). In vitro inoculation experiments showed that strain GYX7 effectively reduced the incidence and severity of walnut anthracnose. These findings suggest that strain GYX7 has the potential to be a biocontrol agent for managing walnut anthracnose. Reference | Related Articles | Metrics | Comments（0）

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Research Advances in Diapause of Predatory Stink Bugs (Hemiptera) XU Bowen, LI Yuyan, HE Weiwei, XUE Chuanzhen, SHEN Zhongjian, MAO Jianjun, ZHANG Lisheng, WU Huihui Chinese Journal of Biological Control    2025, 41 (1): 193-206.   DOI: 10.16409/j.cnki.2095-039x.2024.03.017 Abstract （512）      PDF（pc） （530KB）（3961）       Knowledge map    Save The predatory stink bugs are economically important natural enemy insects and are effective in the biological control of agricultural and forestry pests. Diapause is a physiological adaptation strategy that insects can enter a developmental arrest state in response to unfavorable environmental conditions. Many species of predatory stink bugs enter diapause as adults or eggs. Understanding the characteristics and the regulation mechanisms of diapause in predatory stink bugs not only helps to decipher the environmental adaptation mechanism of insect development, but also can prolong the storage period of natural enemies, promote their year round mass production and improve the control efficiency by manipulating the diapause process. It is of great significance to promote the large-scale mass production, conservation and utilization of natural enemy insects. In this review, the diapause characteristics of 27 species of predatory stink bugs were summarized. The biological, ecological and physiological features during diapause of these predatory stink bugs were analyzed. In addition, the molecular regulation mechanism of diapause and the post-diapause developmental biology of the predatory stink bugs were also reviewed. Finally, the application prospect of diapause in the mass rearing of predatory stink bugs and their use in biological control was discussed. This review provides an important reference for in-depth research on the diapause manipulation technique and diapause mechanism in predatory stink bugs, and for promotion of their mass production, storage and application. Reference | Related Articles | Metrics | Comments（0）

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Biocontrol Effects of Bacillus amyloliquefaciens HN11 in Combination with Tobacco Waste Organic Fertilizers on Strawberry Root Rot LIU Miaojiao, LIN Sukun, LIU Rong, ZHANG Junhui, LI Lin, YI Ting, XU Hanhong, ZENG Lingda Chinese Journal of Biological Control    2025, 41 (1): 143-155.   DOI: 10.16409/j.cnki.2095-039x.2025.02.009 Abstract （396）      PDF（pc） （3844KB）（3935）       Knowledge map    Save This study investigates an environmentally friendly approach to control strawberry root rot using biocontrol agents. Laboratory experiments revealed that B. amyloliquefaciens HN11 exhibits antagonistic activity against various soil-borne pathogens, particularly disrupting the mycelial structure of F. oxysporum, the primary pathogen causing strawberry root rot, and interfering with its physiological metabolism. In pot experiments, the combination of HN11 and tobacco waste organic fertilizer achieved a disease control efficacy of 75.00% against strawberry root rot. The organic fertilizer enhanced the colonization ability of HN11, maintaining its population at 104-105 CFU/g in soil and rhizosphere even 30 days after application. Field trials further demonstrated that applying HN11 via root drench at 75 L/h2 in conjunction with 6000 kg/hm2 of tobacco waste organic fertilizer resulted in the best disease control efficacy of 73.85%, while also promoting strawberry growth. High-throughput sequencing analysis indicated that this combined application optimized the soil microbial community structure, increasing the relative abundance of beneficial genera such as Bacillus and Pseudomonas, while reducing the relative abundance of Ascomycota. Overall, the integration of HN11 with tobacco waste organic fertilizer offers a promising green strategy for the management of strawberry root rot. Reference | Related Articles | Metrics | Comments（0）

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Screening of Endophytic Fungi of Andrographis paniculata Resistant to Ralstonia solanacearum Infecting Peanuts and Study of Their Inhibitory Components HUANG Jin, HU Jinwen, DING Mei, YANG Fan, LUO Wenrong, DU Qin Chinese Journal of Biological Control    2025, 41 (2): 396-410.   DOI: 10.16409/j.cnki.2095-039x.2025.02.028 Abstract （664）      PDF（pc） （5856KB）（3866）       Knowledge map    Save To identify potential biocontrol strains against peanut bacterial wilt caused by Ralstonia solanacearum, we screened endophytic fungi of Andrographis paniculata leaves using the Oxford cup inhibition method. A strain with strong inhibitory activity against R. solanacearum was isolated and identified as Colletotrichum sp. CXL-11 based on morphological characteristics and 16S rDNA phylogenetic analysis. We further investigated its antibacterial mechanism through co-culture experiments and analyzed the antifungal components in its fermentation extract using LC-MS metabolomics. The bioactivity of selected metabolites against R. solanacearum was also validated. The results showed that the fermentation extract of CXL-11 produced an inhibition zone of 19.08 mm against R. solanacearum, with a minimum inhibitory concentration (MIC) of 0.625 mg/mL. The extract disrupted the membrane integrity of R. solanacearum, causing surface shrinkage and deformation of bacterial cells, and induced oxidative stress through the accumulation of reactive oxygen species. Metabolomic analysis revealed that the primary antifungal components were polyketides and coumarins, including isogentisin, bergapten, scopoletin, scopolin, and esculetin, all of which exhibited inhibitory effects against R. solanacearum. The biosynthesis pathway of cofactors was identified as a key mechanism underlying the antibacterial activity of CXL-11. In conclusion, the endophytic fungus Colletotrichum sp. CXL-11 demonstrates significant potential as a biocontrol agent for peanut bacterial wilt. This study provides theoretical support for the development of natural antimicrobial agents and highlights the promising application of CXL-11 in sustainable disease management. Reference | Related Articles | Metrics | Comments（0）

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Initial Exploration of Cordyceps javanica and Neoseiulus barkeri Combined Control Technology for Tetranychus urticae ZHAO Yu, MAO Ruixia, XING Peixiang, DIAO Hongliang, LI Xianwei, MA Ruiyan Chinese Journal of Biological Control    2024, 40 (6): 1250-1259.   DOI: 10.16409/j.cnki.2095-039x.2024.11.001 Abstract （511）      PDF（pc） （1625KB）（3485）       Knowledge map    Save To assess the potential applications of the Cordyceps javanica IJ-tg19 combined with Neoseiulus barkeri for the control of the Tetranychus urticae, the virulence of strain IJ-tg19 against T. urticae and the N. barkeri was determined in indoor experiments. Subsequently, greenhouse and field trials were conducted to evaluate the efficacy of the fungus combined with N. barkeri in controlling T. urticae. The results showed that under the treatment of a spore suspension with a concentration of 1×108 spores/mL, the strain IJ-tg19 had a half-lethal time (LT50) of 3.23 days for the adult stage of the T. urticae, and a lethal concentration (LC50) of 1.67×105spores/mL at 5 days after inoculation. Both biological control measures, whether applied individually or in combination, were effective in controlling the T. urticae, but the combined treatment had a higher control efficacy (96.74%, 98.90%). In the field application trial of “C. javanica-N. barkeri” in the control and treatment areas, there was a significant difference in the population density of the T. urticae. The T. urticae was detected 24 days earlier in the control area compared to the treatment area. The peak population densities in the control area and treatment area were 102 mites/leaf (29, November) and 7.3 mites/leaf (5, December) respectively. This study demonstrates that C. javanica IJ-tg19 combined with N. barkeri is more effective than their individual application, which highlights the potential of the application of fungus and N. barkeri for the prevention and biological control of greenhouse T. urticae. Reference | Related Articles | Metrics | Comments（0）

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Screening of Myxobacterial Strains for Biocontrol of Cotton Verticillium Wilt and Their Biocontrol Potential PAN Wen, DOU Xinyu, LUO Ming, MA Deying, LU Wei, HAN Jian Chinese Journal of Biological Control    2024, 40 (5): 1074-1087.   DOI: 10.16409/j.cnki.2095-039x.2024.02.049 Abstract （368）      PDF（pc） （3116KB）（3473）       Knowledge map    Save To explore biocontrol resources for cotton Verticillium wilt and evaluate the potential application of myxobacteria in managing this disease, we utilized antagonistic activity of mycobacterial strains previously isolated and preserved in our laboratory against Verticillium dahlia were assessed using confrontation culture, mixed culture and dual culture methods, and strains with strong antagonistic effects selected. The efficacy of mycobacterial fermentation broth and solid bacterial formulations against cotton Verticillium wilt was evaluated under greenhouse conditions, along with their impact on cotton growth. Morphological and molecular biological techniques were employed to identify the biocontrol potential of the selected mycobacterial strains. The results demonstrated that the four selected mycobacterial strains effectively preyed on V. dahliae hyphae, inhibited spore germination, and exhibited antibacterial activities through multiple mechanisms. Pot experiments revealed that the fermentation broth of these strains had control effects on cotton Verticillium wilt, with the efficacies ranging from 11.56% to 38.23%. However, efficacies of solid bacterial formulations of strains KS01, NSE37, NSE41 and KY01 on cotton Verticillium wilt were 79.83%, 77.02%, 66.57% and 58.99%, respectively. Based on morphological and molecular analyses, the strains KS01 and KY01 were identified as Myxococcus sp., and strains NSE41 and NSE37 as Cystobacter sp.. The above results showed that four mycobacterial strains had biocontrol potential against cotton Verticillium wilt. Reference | Related Articles | Metrics | Comments（0）

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Study on the Function of ecp Gene Cluster in Colonization of Pine Wood Nematode by Enterobacter Ludwigii AA4 WANG Mingyue, HE Shan, ZHANG Lixia, WANG Sijia, YUAN Zhibo, WU Di, WANG Chuanzhen, PAN Jialiang, BAO Shengbo, WANG Qi, NIU Ben Chinese Journal of Biological Control    2024, 40 (5): 1022-1035.   DOI: 10.16409/j.cnki.2095-039x.2024.01.006 Abstract （433）      PDF（pc） （2997KB）（3276）       Knowledge map    Save Enterobacter ludwigii AA4, a biocontrol bacterial strain, can efficiently colonize and kill pine wood nematode (PWN) to prevent the occurrence of pine wilt disease (PWD). Escherichia coli common pilus (ECP) can promote bacterial colonization by enhancing the bacteria-host interactions and bacterial interspecies interplay within the biofilms. The ECP is encoded by the ecp gene cluster. In order to explore the function of ECP in colonization of PWN by strain AA4, we identified the ecp gene cluster in AA4 by using BLAST alignment. By employing the Red (λ, β, exo)-mediated DNA homologous recombination technology, we further constructed the ecp gene cluster knock-out deletion mutant and analyzed its ability to colonize the PWN plus the colonization-related phenotypes. The outcomes showed that the pilus assembly, motility, biofilm formation of the knock-out mutant and its residence on the PWN was significantly reduced. Thus, ecp gene cluster may regulate the colonization of the PWN by E. ludwigii AA4 through affecting its motility and biofilm formation via modulation of its pilus synthesis. Altogether, our results will serve as theoretical bases for revealing the molecular mechanisms underlying the PWN colonization by strain AA4 and will provide scientific evidence guiding the development of biopesticides for controlling the PWD and their efficient application. Reference | Related Articles | Metrics | Comments（0）

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Analyses of Transcriptome Expression Profiles during Interaction between Trichoderma longibrachiatum and Aspergillus flavus QI Ting, WANG Xinyu, HUANG Shihui, HU Fengbin, XU Tingting, LIANG Caikang, WU Lijuan, NIU Xi, RAN Xueqin, WANG Jiafu Chinese Journal of Biological Control    2025, 41 (2): 321-334.   DOI: 10.16409/j.cnki.2095-039x.2025.02.021 Abstract （392）      PDF（pc） （2907KB）（3073）       Knowledge map    Save To clarify the molecular antagonistic mechanism of Trichoderma inhibiting the growth of Aspergillus flavus, colonies of T. longibrachiatum and A. flavus were collected during their interaction periods, and the enzymes activities to degrade cell wall and antioxidant were detected via ELISA. The differentially expressed genes (DEGs) and the biological processes were further analyzed during the interaction between two fungi using RNA-seq method. The results indicated that the colonies growth rates of T. longibrachiatum were significantly faster with a plate inhibiting rate against A. flavus of 81.1% at the same environment. T. longibrachiatum generated larger amounts of cellulase, chitinase and β-1,3-glucanase. Moreover, the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were suppressed, while the content of malondialdehyde (MDA) was boosted in A. flavus. Compared with control groups, those DEGs were detected to be 2202, 755 and 789 in A. flavus, and 8507, 3055 and 1363 in T. longibrachiatum at early, middle and late stages, respectively. And eleven pivotal interacting candidate genes were screened based on the transcriptomic analysis. These candidate genes are mainly involved in biological processes such as cell wall synthesis, cell membrane permeability, redox reaction, etc. It suggested that T. longibrachiatum could inhibit the effects of antioxidant system and antagonize the growth of A. flavus by up-regulating genes coding for enzymes to degrade cell wall and cell membrane, and by inhibiting the expression of heat shock protein genes in A. flavus. The results would provide a theoretical foundation to take T. longibrachiatum as a biocontrol resource against A. flavus and the production of aflatoxin. Reference | Related Articles | Metrics | Comments（0）

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Preparation of Wettable Powder Formulation of Trichoderma harzianum M-17 Chlamydospores and Its Field Control Effect on Potato Dry Rot WANG Xigang, GUO Chengjin, JIAO Yang, ZHAO Pei, TIAN Jing, ZHANG Lirong, SHEN Ruiqing Chinese Journal of Biological Control    2024, 40 (6): 1319-1330.   DOI: 10.16409/j.cnki.2095-039x.2024.02.061 Abstract （716）      PDF（pc） （576KB）（3040）       Knowledge map    Save To development an efficient biocontrol antimicrobial agent of potato dry rot and prolong the shelf of the product, this study utilized Trichoderma harzianum M-17 as the primary research object. Single factor selection and formulation optimization were used to obtain the wettable powder (WP). The optimal formulation of WP was chlamydospore powder (20%), attapulgite (68%) as carrier, sodium dodecyl sulfonate (4%) as wetting agent, sodium carboxylcellulose (7%) as dispersion, and ascorbic acid (1%) as UV protective agent. The chlamydospore content of this WP was 3.1×108 CFU/g, the wetting time was 56.5 s, the spore suspension rate was 82.27%, pH 6.79, the moisture content was 2.26%, the ratio transiting standard test sieve with 74 µm was 98%. All indicators meet the national standards. The results of field experiments showed that T. harzianum M-17 chlamydospore wettable powder could promote the growth of potato plants and indicated a good control effect on potato dry rot, with a control efficacy of 70.32% and a yield increase rate of 19.03%. This study lays a technical foundation for the development of T. harzianum M-17 chlamydospore wettable powder into a microbial fungicide for the control of potato soil-borne diseases. Reference | Related Articles | Metrics | Comments（0）

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Insecticidal Activity of Extracts from 77 Species of Plants YANG Xuetao, WANG Xiuping, PENG Fei, CHENG Caihong, WU Shengyong, XIE Haicui, YANG Yuedong Chinese Journal of Biological Control    2024, 40 (5): 987-999.   DOI: 10.16409/j.cnki.2095-039x.2023.11.014 Abstract （617）      PDF（pc） （451KB）（3037）       Knowledge map    Save In order to evaluate the insecticidal activity of 77 species of plants belonging to 39 families, leaf disc spray method, immersion method and spray method were used to determine the insecticidal activity of plant ethanol extracts against Tetranychus urticae, Frankliniella occidentalis (Pergande) and Sitobion avenae (Fabricius), and further determine the efficacy of plants with better insecticidal activity against T. urticae, F. occidentalis and S. avenae. The results showed that Atropa belladonna L., Sphagneticola calendulacea, Hypecoum erectum L., Smilaz china L., Hippophae rhamnoides L., Dianthus chinensisL., Campsis grandiflora and Eupatorium japonicum extracts had high toxic on T. cinnabarinus, with the corrected mortality of more than 70% after 72 h; and the LC50 value was 254, 297, 413, 424, 428, 553, 663, 725 mg/mL, respectively. A. belladonna L. and S. calendulacea extracts had high toxic on F. occidentalis, with the corrected mortality of more than 80% after 72 h; and the LC50 value was 272, 306 mg/mL, respectively. S. china, H. erectum, H. rhamnoides, Incarvillea sinensis Lam., C. grandiflora and E. japonicum extracts had high toxic on F. occidentalis, with the corrected mortality of 70%—80% after 72 h; and the LC50 value was 446, 485, 523, 563, 710, 712 mg/mL, respectively. Reynoutria japonica Houtt.,A. belladonna, Rubus crataegifolius Bge, Kochia scoparia and Stellera chamaejasme L. extracts had high toxic on S. avenae, with the corrected mortality of more than 70% after 72 h; and the LC50 value was 281, 336, 540, 657, 730 mg/mL, respectively. Plants such as A. belladonna, R. japonica, S. calendulacea and S. china have high corrected mortality rates against different tested insects, demonstrating potential insecticidal activity, which is worthy of further study. Reference | Related Articles | Metrics | Comments（0）

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Isolation and Identification of Burkholderia cenocepacia YM12 and Its Control Effect on Cucumber Soft Rot Disease WEI Xinchen, WANG Qiao, SHI Bin, XIE Xuewen, SHI Yanxia, CHAI Ali, LI Baoju, LI Lei Chinese Journal of Biological Control    2024, 40 (5): 1088-1098.   DOI: 10.16409/j.cnki.2095-039x.2024.02.059 Abstract （687）      PDF（pc） （3849KB）（2999）       Knowledge map    Save Soft rot of cucumber is a bacterial disease caused by Pectobacterium brasiliense. The disease is serious and difficult to control. In order to screen biocontrol microorganisms with high antibacterial effect, a strain YM12 with significant inhibitory effect on P. brasiliense was isolated from cucumber rhizosphere soil. Through morphological observation, physiological and biochemical determination and multi-gene phylogenetic tree analysis, the strain was identified as Burkholderia cenocepacia. Analysis of antibiotic synthesis-related genes showed that strain YM12 might also produce Pyrrolnitrin and Pyhluteorin. Analysis of biocontrol traits characteristics confirmed that YM12 had the ability to produce phosphatase protease and pyoverdine. The strain had antagonistic effects on 5 pathogenic bacteria and 3 pathogenic fungi by antimicrobial spectrum analysis. Pot experiment showed that strain YM12 had obvious control effect on cucumber soft rot, with the efficacy of 62.84%.In summary, strain YM12 is the first reported B. cenocepacia with biocontrol effect on bacterial soft rot, which has high biocontrol research value and development and application potential. Reference | Related Articles | Metrics | Comments（0）

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Entomogenous Fungus Diversity of Four Habitats in Jiulong Mountain Nature Reserve ZHAO Yuxia, CHEN Lihua, JIA Xiaoqi, SHAO Zhaoyang, SHU Jinping, ZHANG Yabo Chinese Journal of Biological Control    2024, 40 (5): 1135-1148.   DOI: 10.16409/j.cnki.2095-039x.2024.02.057 Abstract （351）      PDF（pc） （3492KB）（2985）       Knowledge map    Save In this study, soil samples from four different habitats (tea garden, sweet potato field, plantation and primary forest) in Jiulongshan National Nature Reserve of ZheJiang Province were used as research materials. Effective data were obtained by illumina NovaSeq sequencing platform for analysis of the fungal diversity and community structure of entomogenous fungi in 20 soil samples under four different habitats, and the correlation between entomogenous fungus and soil environmental factors, The main purpose was to clarify the diversity and distribution characteristics of soil entomogenous fungus in different habitats and provide scientific basis for the development and utilization of entomogenous fungus and species diversity in Jiulongshan National Nature Reserve, Zhejiang Province. The results showed that the relative abundance of entomogenous fungus in forests (primary forest, plantation) was higher than that in agricultural land (tea garden, sweet potato field) under four habitats. There was a significant difference in the entomogenous fungus communities in the soils of the four habitats. The dominant genera in the primary forest and plantation were Tolypocladiumi, while those in the tea garden and sweet potato field were Fusarium and Metarhizium respectively. The ratio of carbon to nitrogen and total nitrogen in soils were significant environmental factors affecting the entomogenous fungus in the region. In forest soils, entomogenous fungus floras was positively correlated with soil carbon to nitrogen ratio and total nitrogen level, while in agricultural soils, the contents of total phosphorus and available phosphorus were positively correlated with entomogenous fungus communities. Among the top ten genera in the relative abundances of soil entomogenous fungi in four habitats, Metarhizium, Isaria and Cordyceps were significantly positively correlated with soil carbon to nitrogen ratio and total nitrogen level, while Fusarium was significantly negatively correlated with soil carbon to nitrogen ratio. The results showed that there were rich entomogenous fungi species in Jiulongshan Nature Reserve, habitat type influenced the abundance of entomogenous fungi, and the relative abundance of entomogenous fungi in the soils of forest was higher than that in the four different habitats. The dominant entomogenous fungi mainly concentrated in three genera, such as Metarhizium, Tolypocladium and Fusarium. Reference | Related Articles | Metrics | Comments（0）

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Screening of Antifungal Activity of Extracts from 13 species of Plant against Three Wheat Pathogens and Analysis of Their Constituents YAN Bin, LIANG Jing, GONG Jingyun, ZHOU Xiaoyun, HU Linfeng, ZHANG Jungao, LEI Bin, LI Kemei Chinese Journal of Biological Control    2025, 41 (3): 605-616.   DOI: 10.16409/j.cnki.2095-039x.2025.02.032 Abstract （357）      PDF（pc） （1643KB）（2947）       Knowledge map    Save Screening and evaluating the antibacterial activity of Xinjiang's plant resources provides a foundation for the development of botanical pesticides. In this study, the antifungal activities of 13 plant extracts from Xinjiang were screened and measured through the mycelial growth rate method, targeting three wheat pathogens: Bipolaris sorokiniana, Fusarium graminearum, and Microdochium nivale. Additionally, the main components of Rumex crispus were analyzed using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The organic solvent extracts from 13 plant species showed different inhibitory activities against three wheat pathogenic fungi, and the antibacterial efficacy increased with increasing concentration. Among them, the methanol extract of Rumex crispus exhibited the strongest inhibitory activity against B. sorokiniana, with an EC50 value of 6.380 mg/mL; the methanol extract of Salsola collina demonstrated the strongest inhibitory activity against F. graminearum, with an EC50 value of 26.362 mg/mL; and the ethanol extract of R. crispus showed the strongest inhibitory activity against Microdochium nivale, with an EC50 value of 8.203 mg/mL. A total of 42 chemical components were identified in the R. crispus extract, primarily flavonoids, fatty acyls, carboxylic acids, and their derivatives, accounting for 66.67% of the total. It can be seen from this that the methanol extract of R. crispus has good antibacterial activity and is worth further study. Reference | Related Articles | Metrics | Comments（0）

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Synergistic Effect of Compound Seed Coating Containing Biocontrol Bacteria ZF516 and Chemical Agent on the Control of Cowpea Fusarium Root Rot ZHAO Zixuan, SHI Bin, LANG Junying, JIANG Tao, YANG Jingjing, FAN Tengfei, XIE Xuewen, SHI Yanxia, CHAI Ali, LI Baoju, LI Lei Chinese Journal of Biological Control    2025, 41 (1): 165-176.   DOI: 10.16409/j.cnki.2095-039x.2025.02.010 Abstract （463）      PDF（pc） （3446KB）（2753）       Knowledge map    Save In order to obtain biocontrol bacteria with remarkable antagonistic effects against cowpea root rot, in this study, the dural culture method was utilized to isolate a biocontrol strain, ZF516, which exhibited significant inhibitory effects on the complex pathogenic bacteria of cowpea root rot. Subsequently, the morphological characteristics of this strain were analyzed, and the 16S rRNA, gyrA, and rpoA gene sequences were compared and analyzed to clarify the taxonomic status of strain ZF516. The biosafety and root rot control efficacy of cowpea were evaluated through compatibility and compounding ratio screening of ZF516 with 23% imidacloprid·fludioxonil·difenoconazole and 29% thiamethoxam·fludioxonil·metalaxyl-M, respectively. The results indicated that the inhibition rates of strain ZF516 against Fusarium solani, F. oxysporum, and F. proliferatum were 71.83%, 66.31%, and 73.73%. The strain ZF516 was identificated as Bacillus velezensis. After formulating Bacillus velezensis ZF516 and 23% imidacloprid- thiamethoxam-benomyl at a volume ratio of 7:3, the emergence rate of cowpea seeds treated with this combined seed coating was significantly higher compared to untreated seeds and seeds treated with each agent individually. Additionally, the compound of Bacillus velezensis ZF516 with 23% imidacloprid·thiamethoxam·benomyl suspension seed coating agent showed a synergistic effect on the control of cowpea root rot, achieving a control efficiency of up to 67.56%, which surpassed the efficacy observed with the chemical seed coating agent alone. In this study, a strain of Bacillus velezensis ZF516 was isolated and characterized. ZF516 exhibited significant disease resistance against cowpea root rot, both as a standalone treatment and in combination with chemical agents. This study provides essential strain resources and integrated control strategies for the development of biological seed coating agents aimed at managing cowpea root rot. Reference | Related Articles | Metrics | Comments（0）