一株疫病拮抗青霉P. st10 菌株的抗菌活性及其对辣椒疫病的盆栽防效

一株疫病拮抗青霉P. st10 菌株的抗菌活性及其对辣椒疫病的盆栽防效

马　艳¹;常志州¹;赵江涛²;黄红英¹;叶小梅¹;张建英¹

1. 江苏省农科院农业资源与环境研究所, 南京　210014 ;2. 江苏省农科院科研处

收稿日期:2005-09-13 修回日期:1900-01-01 出版日期:2006-08-08 发布日期:2006-08-08
通讯作者: 常志洲

Antifungal Activity of Penicillium striatisporum Strain P. st10 and Its Inhibitionagainst Pepper Blight Caused by Phytophthora spp.

MA Yan;CHANG Zhi-zhou;ZHAO Jiang-tao

Institute of Agricultural Resources and Environments , Jiangsu Academy of Agricultural Sciences , Nanjing 210014 , China

Received:2005-09-13 Revised:1900-01-01 Online:2006-08-08 Published:2006-08-08
Contact: CHANG Zhi-zhou

摘要/Abstract

摘要： 筛选得到一株疫霉菌的拮抗青霉Penicillium striatisporum P. st10。该菌株强烈抑制疫霉
菌及核盘菌的菌丝生长。无菌滤液抑制辣椒疫霉孢子囊的形成及孢子囊、游动孢子的萌发,且
能杀死菌丝细胞。该菌株在辣椒根际有很强的定殖能力,有机肥对其定殖有增强作用,接种后
40d 在根际的青霉菌数量,青霉处理为0176 ×10⁴cfu/ g ,而青霉菌肥处理为2.55 ×10⁴cfu/ g。盆栽试验显示:处理7d 后,土壤只接种辣椒疫霉对照发病率为93 %;同时接种青霉培养滤液和辣椒疫霉处理为16 %;接种青霉培养滤液、新鲜菌丝和辣椒疫霉处理为15 %。

Abstract: Penicillium striatisporum strain P. st10 was showed to be highly antagonistic against mycelial growth of Phytophthora spp. and Sclerotinia sclerotiorum , while not antagonistic to other tested plant pathogens in plate tests. Investigation of antagonistic mechanism under light microscope showed that the dilution of filtrate from liquid fermentation of the fungus inhibited pathogen by leading to hyphal abnor-mality , inhibiting germination of spores , and suppressing formation and germination of sporangium of P.capsici . P. striatisporum could colonize in pepper rhizosphere. Organic fertilizer could improve the colo-nization ability. The rate of pepper blight disease reduced notably when soil was treated with the mycelium of the strain and its filtrate in pot test.

中图分类号:

马　艳1 ;常志州1 ;赵江涛2 ;黄红英1 ;叶小梅1 ;张建英1 . 一株疫病拮抗青霉P. st10 菌株的抗菌活性及其对辣椒疫病的盆栽防效[J]. , DOI: .

MA Yan;CHANG Zhi-zhou;ZHAO Jiang-tao. Antifungal Activity of Penicillium striatisporum Strain P. st10 and Its Inhibitionagainst Pepper Blight Caused by Phytophthora spp.[J]. , DOI: .