红树内生细菌菌株Kc-38的抗菌物质及对采后芒果炭疽病的防效

红树内生细菌菌株Kc-38的抗菌物质及对采后芒果炭疽病的防效

汪远¹, 詹儒林², 何红¹, 吕柱彬¹, 余小琴¹, 梁永琪¹, 陈鸿宇¹

1. 广东海洋大学农学院, 湛江 524088;2. 中国热带农业科学院南亚热带作物研究所, 湛江 524091

收稿日期:2010-04-25 修回日期:1900-01-01 出版日期:2011-01-18 发布日期:2011-01-18

Antibiotic Substances Produced by Mangrove Endophytic Bacteria Kc-38 and Biocontrol Efficacy on Anthracnose of Postharvest Mangoes

WANG Yuan¹, ZHAN Ru-lin², HE Hong¹, Lü Zhu-bin¹, YU Xiao-qin¹, LIANG Yong-qi¹, CHEN Hong-yu¹

1. Agricultural College of Guangdong Ocean University, Zhanjiang 524088;2. South Asia Tropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China

Received:2010-04-25 Revised:1900-01-01 Online:2011-01-18 Published:2011-01-18

摘要/Abstract

摘要： Kc-38菌株是本实验室从红树植物秋茄Kandelia candel体内分离得到的内生细菌,对峙生长法测定发现该菌株对芒果炭疽病菌Colletotrichum gloeosporioides和枯萎病菌Fusarium oxysporum等多种植物病原菌具有较强的抑制作用。根据菌体形态、培养特性、生理生化反应及16S rDNA序列分析将该菌株初步鉴定为解淀粉芽孢杆菌Bacillus amyloliquefaciens。该菌株对芒果炭疽病具有良好的防效,用菌株发酵液及发酵上清液处理9d后,对采后芒果炭疽病的防效分别达到65.12%和62.79%。进一步研究表明,其分泌的胞外抗菌物质为一类对热稳定和抗紫外照射的蛋白类物质,在pH2.0~11.0范围内均具有抑菌作用,以pH2.0~8.0范围内的抗菌活性较强。

Abstract: The endophytic bacterial strain Kc-38 isolated from the tissue of Kandelia candel had strong inhibitory activity against many plant pathogenic fungi, such as Colletotrichum gloeosporioides, Fusarium oxysporum, and so on. According to the morphological, physiological and biochemical characteristics, and 16S rDNA sequence analysis, strain Kc-38 was identified as Bacillus amyloliquefaciens. The strain effectively suppressed mango anthracnose disease caused by C. gloeosporioides. When tested on postharvest mango fruits and treated with the bacterial suspension and its cellular exudates, the control efficacy on the 9^th d reached 65.12% and 62.79%, respectively. Further studies showed that the antibiotic substances produced by the strain were some kind of antifungal proteins. The proteins were demonstrated to be UV-tolerant, thermostable and inhibitory at pH value ranged from 2.0 to 11.0, but more inhibitory at pH 2.0-8.0 than at pH9.0-11.0.

中图分类号:

汪远1 , 詹儒林2 , 何红1 , 吕柱彬1 , 余小琴1 , 梁永琪1 , 陈鸿宇1 . 红树内生细菌菌株Kc-38的抗菌物质及对采后芒果炭疽病的防效[J]. , DOI: .

WANG Yuan¹, ZHAN Ru-lin², HE Hong¹, Lü Zhu-bin¹, YU Xiao-qin¹, LIANG Yong-qi¹, CHEN Hong-yu¹. Antibiotic Substances Produced by Mangrove Endophytic Bacteria Kc-38 and Biocontrol Efficacy on Anthracnose of Postharvest Mangoes[J]. , DOI: .

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