太子参叶斑病抗性诱导因子筛选

太子参叶斑病抗性诱导因子筛选

林丛发, 翁琳琳, 钟爱清, 陈芦根

福建省宁德市农业科学研究所, 福安 355017

收稿日期:2010-10-24 修回日期:1900-01-01 出版日期:2011-08-25 发布日期:2011-08-25

Screening of Activators Inducing Resistance on Leaf Spot Disease (Phyllosticta commonsii) in Taizishen (Pseudostellaria heterophylla)

LIN Cong-fa, WENG Lin-lin, ZHONG Ai-qing, CHEN Lu-gen

Ning De Agriculture institute, Fu'an 355017, China

Received:2010-10-24 Revised:1900-01-01 Online:2011-08-25 Published:2011-08-25

摘要/Abstract

摘要： 为筛选诱导太子参产生对叶斑病抗性的诱导因子, 在离体培养条件下, 用水杨酸、草酸、壳聚糖、磷酸氢二钾、亚硒酸钠和太子参叶斑病粗毒素液这6种因子诱导处理太子参组培苗, 在1~7d内测定叶片相关酶活性的变化。诱导培养7d后, 用太子参叶斑病粗毒素液对组培苗进行离体叶片针刺接种抗性鉴定, 结果表明, 诱导处理后能明显增强太子参叶片内POD、PPO和PAL的活性; 6种因子均能诱导太子参对叶斑病产生抗性, 以190mg·L^-1和380mg·L^-1草酸的诱抗效果较好, 分别达到76.1%和74.7%, 极显著高于其它诱导因子的诱抗效果。

Abstract: To screen activators on leaf spot disease (Phyllosticta commonsii) of Taizishen (Pseudostellaria heterophylla), in vitro-regenerated Taizishen plantlets were treated under aseptic conditions with 6 kinds of inducing factors including salicylic acid, oxalic acid, chitosan, potassium hydrogen phosphate, sodium selenite and crude Phyllosticta commonsii toxin. The activity of related enzymes in leaves 1-7 days after treatment was determined. Disease reaction of the treated plantlets was identified by pin-prick inoculation of detached leaves with crude P. commonsii toxin 7 days after treatment. Results showed that the 6 inducers could significantly enhance enzymes activity of peroxidase, polyphenol oxidase, phenylalanine ammonia lyase in leaves, and could induce resistance to Taizishen leaf spot disease. When Taizishen was induced with oxalic acid at 190 mg·L^-1 and 380 mg·L^-1, the induced resistant effect reached 76.1% and 74.7%, respectively, significantly higher than that of other inducers.

中图分类号:

林丛发, 翁琳琳, 钟爱清, 陈芦根. 太子参叶斑病抗性诱导因子筛选[J]. , DOI: .

LIN Cong-fa, WENG Lin-lin, ZHONG Ai-qing, CHEN Lu-gen. Screening of Activators Inducing Resistance on Leaf Spot Disease (Phyllosticta commonsii) in Taizishen (Pseudostellaria heterophylla)[J]. , DOI: .

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