Abstract: Insecticidal activity of essential oil extracted from fruits of Daucus carota against Aedes albopictus and Culex pipiens quinquefasciatus was investigated by immersion method and the main compounds of the essential oil were qualitatively and quantitatively analyzed with gas chromatography-mass spectrometry. LC₅₀ values of the essential oil against the 4^th instar larvae of A. albopictus and C. pipiens quinquefasciatus at 24 h were 67.87 μg·mL^-1 and 34.12 μg·mL^-1, respectively. Thirty-four compounds were identified in the essential oil, including the main component α-Pinene (54.72%), followed by β-bisabolene (11.35%) and β-asarone (10.14%). Six active compounds were quantitatively determined, including α-pinene (up to 577.46 mg·mL^-1 in the oil), myrcene (80.26 mg·mL^-1), limonene (28.95 mg·mL^-1), β-caryophyllene (24.38 mg·mL^-1), β-pinene (11.59 mg·mL^-1) and γ-terpinen (2.33 mg·mL^-1). The results indicated that the essential oil showed higher larvicidal activity against mosquitoes than many other plant essential oils which have been documented. It might be considered as a potential source for production of efficient and environment-friendly mosquito larvicide.