Abstract: To improve techniques and strategies of Metarhizium anisopliae application as a biological pesticide against grubs in peanut field, we studied the behavior trends and changes of white grubs related to peanut plants and application M. anisopliae in soil. A series of treatment trials contrasting with the fungus, plants, grubs and control each other, were conducted in the plates and in two pools. The results showed that grubs moved slowly in random directions and could reach the maximum distance in the test range in control treatment without applying M. anisopliae and no host plants in soil. Grubs still moved slowly and dispersed randomly in the soil incorporated M. anisopliae conidia. The distribution ratios of grubs at the sides with vs without the fungus were 53.3% vs 46.7% and 54.4% vs 45.6% in the plates and the pool respectively. They were no significant differences. When incorporating M. anisopliae in center soil but not in periphery, 66.7% of the grubs stayed in the central region. Comparatively, when incorporating the fungus in periphery soil but not in center, 38.3% grubs stayed in the central region, and the majority moved into the surrounding area. The difference was significant. It showed the grubs behaved no significantly positive or negative tropism to the applied fungus, or might be weak positive tendency. However, at presence of host peanuts, 65.0% of the grubs moved and tendd to the side of peanut plants and 83.3% of the grubs moved and tended to the side of peanut plants with M. anisopliae soil, significantly higher than the control side in testing plates. The results from pool trials showed that grubs would move and spread to search when there were no host plants around. And they would move significantly towards peanut plants and remain in the plant vicinity when there were host plants around. They were significantly higher than ones in the far periphery from plant, forming aggregate distribution. M. anisopliae seemingly presented a certain prevention from grubs approaching peanut roots.