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球孢白僵菌与两种昆虫生长调节剂对甜菜夜蛾的联合作用

胡安岭1, 田兆丰2, 高希武1, 农向群3, 李永丹1   

  1. 1. 中国农业大学农学与生物技术学院, 北京100193;2. 北京市农林科学院植物保护环境保护研究所, 北京 100197;3. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193
  • 收稿日期:2014-10-13 修回日期:1900-01-01 出版日期:2015-08-08 发布日期:2015-08-08
  • 通讯作者: 高希武,教授,E-mail:gaoxiwu@263.net.cn;李永丹,副教授,E-mail:yongdanli@cau.edu.cn

Combined Effects of Beauveria bassiana and Two Insect Growth Regulators on Beet Armyworm, Spodoptera exigua (Hübner)

HU Anling1, TIAN Zhaofeng2, GAO Xiwu1, NONG Xiangqun3, LI Yongdan1   

  1. 1. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;2. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;3. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2014-10-13 Revised:1900-01-01 Online:2015-08-08 Published:2015-08-08

摘要: 本文研究了球孢白僵菌与昆虫生长调节剂甲氧虫酰肼及氟铃脲对甜菜夜蛾幼虫的联合毒力,结果表明两种昆虫生长调节剂和白僵菌混用增效作用明显。昆虫生长调节剂(浓度均为致死中浓度,甲氧酰肼25×10-6g/L,氟铃脲1000×10-6g/L与球孢白僵菌8.0×107孢子/mL混合使用,体积比4:1时效果最佳,共毒系数甲氧虫酰肼与白僵菌为285,氟铃脲与白僵菌为208。死虫保湿后,95%长出了白僵菌菌丝,甚至可产生孢子;采用扫描电镜对昆虫体壁纵切面进行观察,各处理组甜菜夜蛾幼虫体壁有明显不同。

Abstract: Combined effects of Beauveria bassiana and two insect growth regulators (IGR), methoxyfenozide and hexaflumuron, on beet armyworm larvae, Spodoptera exigua (Hübner), were investigated. An obvious synergism effect of the two IGRs with Beauveria beauveria was observed. When the IGR at LC50 concentrations (25×10-6g/L for methoxyfenozide and 1000×10-6g/L for hexaflumuron) was mixed with B. bassiana at 4:1 (v/v), the mixtures achieved the best efficacy. The co-toxicity co-efficient (CTC) of methoxyfenozide and B. bassiana was 285, that of hexaflumuron and B. bassiana was 208. Scanning electron microscope observation of the longitudinal section revealed obviously different patterns of the beet armyworm larval integument in different treatment groups.

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