苏云金芽胞杆菌谷氨酰胺合成酶基因glnA的转录调控和过表达

doi:10.16409/j.cnki.2095-039x.2019.04.015

中国生物防治学报 ›› 2019, Vol. 35 ›› Issue (4): 563-569.DOI: 10.16409/j.cnki.2095-039x.2019.04.015

苏云金芽胞杆菌谷氨酰胺合成酶基因glnA的转录调控和过表达

杨帆^1,2, 秦嘉欣³, 彭琦², 张杰², 朱延明¹, 宋福平²

1. 东北农业大学生命科学学院, 哈尔滨 150030;
2. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193;
3. 北京理工大学生命学院, 北京 100081

收稿日期:2019-04-03 出版日期:2019-08-08 发布日期:2019-08-10
通讯作者: 朱延明,教授,E-mail:ymzhu2001@neau.edu.cn;宋福平,研究员,E-mail:fpsong@ippcaas.cn。
作者简介:杨帆,硕士研究生,E-mail:YYF0087@163.com
基金资助:
国家重点研发计划（2017YFD0200400）；国家自然科学基金（31530095）

Transcriptional Regulation and Overexpression of glnA Gene in Bacillus thuringiensis

YANG Fan^1,2, QIN Jiaxin³, PENG Qi², ZHANG Jie², ZHU Yanming¹, SONG Fuping²

1. College of Life Science, Northeast Agricultural University, Harbin 150030, China;
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. School of Life Science, Beijing Institute of Technology, Beijing 100081, China

Received:2019-04-03 Online:2019-08-08 Published:2019-08-10

摘要/Abstract

摘要： 氮代谢对细菌生存至关重要，谷氨酰胺和谷氨酸盐在细菌氮代谢中发挥重要作用。本文对苏云金芽胞杆菌谷氨酰胺合成酶基因glnA的转录调控及过表达进行了研究。通过构建glnR基因的缺失突变株及glnA基因启动子融合lacZ基因的表达载体，测定突变株中glnA启动子活性，发现在胞外营养充足的情况下转录因子GlnR负调控glnA基因。同时在glnR基因苏云金芽胞杆菌缺失突变株中过表达glnA基因。发现与野生型相比，过表达菌株杀虫活性得到提高。

关键词: 氮代谢, 谷氨酰胺合成酶, 转录调控

Abstract: Nitrogen metabolism is essential for bacterial survival, while glutamine and glutamate play an important role in bacterial nitrogen metabolism. The transcriptional regulation and overexpression of the glutamine synthetase gene of Bacillus thuringienesis was examined in this study. By constructing the glnR deletion strain and glnA promoter fusion with lacZ gene, the activity of glnA promoter was investigated in the mutant strain and wild type stain. The results showed that glnA gene was negatively regulated by transcriptional factor GlnR in the case of sufficient extracellular nutrition source. Overexpression of glnA gene in glnR mutant increased insecticidal activity compared to the wild type strain.

Key words: nitrogen metabolism, glutamine synthetase, transcriptional regulation

中图分类号:

S476.1

杨帆, 秦嘉欣, 彭琦, 张杰, 朱延明, 宋福平. 苏云金芽胞杆菌谷氨酰胺合成酶基因glnA的转录调控和过表达[J]. 中国生物防治学报, 2019, 35(4): 563-569.

YANG Fan, QIN Jiaxin, PENG Qi, ZHANG Jie, ZHU Yanming, SONG Fuping. Transcriptional Regulation and Overexpression of glnA Gene in Bacillus thuringiensis[J]. Chinese Journal Of Biological Control, 2019, 35(4): 563-569.

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苏云金芽胞杆菌谷氨酰胺合成酶基因glnA的转录调控和过表达

Transcriptional Regulation and Overexpression of glnA Gene in Bacillus thuringiensis

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