菌株CSR-2对玉米茎腐病的生防作用及其全基因组序列分析

doi:10.16409/j.cnki.2095-039x.2021.08.006

中国生物防治学报 ›› 2022, Vol. 38 ›› Issue (1): 242-249.DOI: 10.16409/j.cnki.2095-039x.2021.08.006

菌株CSR-2对玉米茎腐病的生防作用及其全基因组序列分析

赵美茜, 白忠义, 张祥辉, 刘金亮, 潘洪玉, 张艳华

吉林大学植物科学学院, 长春 130062

收稿日期:2021-08-30 出版日期:2022-02-08 发布日期:2022-03-01
通讯作者: 张艳华,女,博士,教授,E-mail:yh_zhang@jlu.edu.cn
作者简介:赵美茜,女,硕士研究生,E-mail:1074439080@qq.com;白忠义,男,实验师,E-mail:baizy@jlu.edu.cn
基金资助:
国家重点研发计划政府间国际科技创新合作重点专项（2019YFE0114200）；吉林省科技发展计划项目（20190201025JC）

Biocontrol Effect of Strain CSR-2 on Maize Stalk Rot and Complete Genome Sequence Analysis

ZHAO Meixi, BAI Zhongyi, ZHANG Xianghui, LIU Jinliang, PAN Hongyu, ZHANG Yanhua

College of Plant Science, Jilin University, Changchun 130062, China

Received:2021-08-30 Online:2022-02-08 Published:2022-03-01

摘要/Abstract

摘要： 本研究从玉米根际土壤中分离获得一株具有良好生防效果的细菌菌株CSR-2，该菌株对多种植物病原真菌均具有拮抗作用，抑制禾谷镰孢菌丝生长、形态及孢子萌发。将菌株CSR-2与玉米膜下滴灌水肥药一体化技术联用田间试验表明，该菌株对玉米茎腐病的防治效果为52.3%，可显著降低玉米茎腐病的田间发病率。利用第三代PacBio测序技术，对菌株CSR-2进行全基因组测序，基因组长度为3202366 bp，共编码3007个基因，平均基因长度为943.76 bp。根据全基因组序列分析，确定菌株CSR-2为醋酸菌中的茂物朝井杆菌Asaia bogorensis（GenBank收录号：CP081941）。该研究为进一步挖掘微生物资源及其代谢基因簇用于农业生物防治提供理论依据。

关键词: 生防菌株, 玉米茎腐病, 禾谷镰孢, 全基因组测序

Abstract: A potential biocontrol strain, CRS-2, was isolated from maize rhizosphere soil. The strain showed antagonism against Fusarium graminearum, Magnaporthe oryzae, Botrytis cinerea, Sclerotinia sclerotiorum, Cercospora zeae, Helminthosporium maydis, and Rhizoctonia solani. The strain CRS-2 could inhibit the mycelial growth, mycelial morphology and spore germination of F. graminearum. The experiment of combined CRS-2 and water and fertilizer under film drip irrigation showed that the field control efficacy of CRS-2 against maize stalk rot was 52.3%. The complete genome sequence of CRS-2 were obtained by the third-generation PacBio sequencing technology. The genome length was 3202366 bp, encoding 3007 genes with average gene length gene was 943.76 bp. A total number of 198692 high-quality long reading fragments were obtained, the circle genome map was constructed, and the annotated coding genes showed that the strain was Asaia bogorensis (GenBank accession number:CP081941). This research provides a theoretical basis for further discovering microbial resources and their metabolic gene clusters for agricultural biological control.

Key words: biocontrol strain, maize stalk rot, Fusarium graminearum, genome sequencing

中图分类号:

S476

赵美茜, 白忠义, 张祥辉, 刘金亮, 潘洪玉, 张艳华. 菌株CSR-2对玉米茎腐病的生防作用及其全基因组序列分析[J]. 中国生物防治学报, 2022, 38(1): 242-249.

ZHAO Meixi, BAI Zhongyi, ZHANG Xianghui, LIU Jinliang, PAN Hongyu, ZHANG Yanhua. Biocontrol Effect of Strain CSR-2 on Maize Stalk Rot and Complete Genome Sequence Analysis[J]. Chinese Journal of Biological Control, 2022, 38(1): 242-249.

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菌株CSR-2对玉米茎腐病的生防作用及其全基因组序列分析

Biocontrol Effect of Strain CSR-2 on Maize Stalk Rot and Complete Genome Sequence Analysis

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