多粘类芽孢杆菌HY96-2 对番茄青枯病的防治作用

多粘类芽孢杆菌HY96-2 对番茄青枯病的防治作用

徐玲;王伟;魏鸿刚;沈国敏;李元广

华东理工大学海洋生化工程研究所/ 生物反应器工程国家重点实验室, 上海　200237

收稿日期:2005-05-08 修回日期:1900-01-01 出版日期:2006-08-08 发布日期:2006-08-08
通讯作者: 王伟

Effect of Paenibacillus polymyxa HY96-2 on Bacterial Wilt of Tomato

XU Ling;WANG ei;WEI Hong-gang;SHEN Guo-min;LI Yuan-guang

Institute of Marine Bioprocess Engineering , State Key Laboratory of Bioreactor Engineering ,East China University of Science and Technology , Shanghai 200237 , China)

Received:2005-05-08 Revised:1900-01-01 Online:2006-08-08 Published:2006-08-08
Contact: WANG Wei

摘要/Abstract

摘要： 室内测定了拮抗菌株HY96-2 对番茄青枯病病原菌Nb-1 的毒力。平板培养法和双层培
养基法结果表明,HY96-2 对Nb-1 具有较好的拮抗作用,其抑制率分别为20.1 %和24.7 %。通过温室盆栽,确定了HY96-2 发酵液及其制成的生防制剂KDLD 对番茄青枯病的最佳防治方法为浸根法。番茄苗浸1 ×10⁵cfu/ ml 的HY96-2 发酵液0.5h ,栽入灭菌土后浇入该HY96-2发酵液100ml ,处理后30d 防效仍高达69.7 %。温室防治试验结果表明,采用浸根法HY96-2 发酵液施用后21d 对番茄青枯病防效可达77.8 %。生防制剂KDLD 稀释100 倍施用后20d 防效可达81.5 %。大田防治试验结果表明,生防制剂KDLD 稀释100 倍后使用,在番茄整个生育期对番茄青枯病都具有很好的防效,在收获末期的防效仍可达81.8 %。

Abstract: The inhibition of biocontrol agent Paenibacillus polymyxa HY96-2 on Ralstonia solanacearum Nb-1 was determined in vitro. Result showed that the inhibition rates were 20.1 % and 24.7 % by dualculture and double layer culture methods , respectively. Greenhouse trials demonstrated that soaking root
was the best way to use the ferment fluid of HY96-2 and its product KDLD to control tomato bacterial wilt disease. Control efficiencies were 77.8 % at 21 days after soaking root with HY96-2 ferment fluid and 81.5 % at 20 days after soaking with 100 times diluted KDLD. Field tests showed that 100 times diluted KDLD prevented the disease in all growing season of tomato with control efficiency 81.8 % at harvest.

徐玲;王伟;魏鸿刚;沈国敏;李元广. 多粘类芽孢杆菌HY96-2 对番茄青枯病的防治作用[J]. , DOI: .

XU Ling;WANG ei;WEI Hong-gang;SHEN Guo-min;LI Yuan-guang. Effect of Paenibacillus polymyxa HY96-2 on Bacterial Wilt of Tomato[J]. , DOI: .