暹罗芽胞杆菌JZ1-4-10对马铃薯干腐病的抑菌作用及机理

doi:10.16409/j.cnki.2095-039x.2024.02.019

中国生物防治学报 ›› 2024, Vol. 40 ›› Issue (3): 641-651.DOI: 10.16409/j.cnki.2095-039x.2024.02.019

• 研究论文 • 上一篇

暹罗芽胞杆菌JZ1-4-10对马铃薯干腐病的抑菌作用及机理

蒙耀, 吴慧芳, 魏琳, 石晖琴, 沈硕

青海大学/青海省农林科学院/青藏高原生物技术教育部重点实验室/青海省马铃薯育种重点实验室/西北马铃薯教育部工程研究中心, 西宁 810016

收稿日期:2023-08-04 发布日期:2024-06-07
通讯作者: 沈硕,博士,研究员,E-mail:fjfzss@126.com。
作者简介:蒙耀,男,硕士研究生,E-mail:1810660210@qq.com

Antibacterial Effect and Mechanism of Bacillus siamensis JZ 1-4-10 on Dry Rot Disease of Potato

MENG Yao, WU Huifang, WEI Lin, SHI Huiqin, SHEN Shuo

Qinghai University/Qinghai Academy of Agriculture and Forestry Sciences/The Tibet Plateau Biotechnology Key Laboratory of Ministry of Education/Key Laboratory of Potato Breeding of Qinghai Province/Northwest Potato Engineering Research Center, Ministry of Education, Xining 810016, China

Received:2023-08-04 Published:2024-06-07
Contact: 青海省应用基础研究（2023-ZJ-702);2022年度青海省“昆仑英才?高端创新创业人才”培养领军人才项目（2022）
Supported by:
10.16409/j.cnki.2095-039x.2024.02.019

摘要/Abstract

摘要： 为了得到对马铃薯干腐病具有良好抑菌效果的生防细菌，本研究从4株芽胞杆菌中筛选出暹罗芽胞杆菌JZ1-4-10，并通过马铃薯活体试验探究菌株JZ1-4-10防治马铃薯干腐病及其相关防御酶活性的影响。结果表明，4株芽胞杆菌中菌株JZ1-4-10的抑菌活性最高，对茄病镰刀菌的抑菌率达59.15%，发酵液的抑菌率达61.25%；此外，菌株JZ1-4-10处理有效提高了马铃薯SOD和POD活性，降低PPO酶活性和MDA含量，保持了马铃薯贮藏期间的品质，且对马铃薯干腐病的预防效果优于治疗、钝化的防治效果，达85.17%；同时，该菌株菌悬液及挥发性有机物均有较高的抑菌活性，并具有较强的生物膜形成能力。因此，暹罗芽胞杆菌JZ1-4-10对马铃薯干腐病有着巨大的防治潜力，可进一步开发成生防菌剂。

关键词: 茄病镰刀菌, 暹罗芽胞杆菌, 马铃薯干腐病, 抑菌机理

Abstract: In order to obtain biocontrol bacteria with good antagonistic effect against potato dry rot, we screened a Bacillus siamensis strain JZ1-4-10 from four Bacillus strains and investigate effects of fermentation solution of strain JZ1-4-10 on potato dry rot and related defense enzyme activity. Indoor biocontrol experiments showed that among the 4 strains, strain JZ1-4-10 demonstrated the highest antifungal activity, with the inhibition rate of 59.15%, and its fermentation solution exhibited inhibition rates of 61.25% against Fusarium solanum. In addition, strain JZ1-4-10 treatment effectively improved the potato defense enzyme SOD and POD activities, reduced PPO enzyme activity and MDA content, and maintained the quality of potato during storage. The prevention mode effect of potato dry rot was better than that of the treatment mode and deactivation mode, which was up to 85.17%. Moreover, its bacterial suspension and volatile organic compounds had high antibacterial activity, and the strain had strong biofilm formation ability. Thus, B. siamese JZ1-4-10 showed great potential for inhibiting potato dry rot and could be further developed into biocontrol agent.

Key words: potato dry rot, Bacillus siamensis, Fusarium solanum, antifungal mechanism

中图分类号:

S476

蒙耀, 吴慧芳, 魏琳, 石晖琴, 沈硕. 暹罗芽胞杆菌JZ1-4-10对马铃薯干腐病的抑菌作用及机理[J]. 中国生物防治学报, 2024, 40(3): 641-651.

MENG Yao, WU Huifang, WEI Lin, SHI Huiqin, SHEN Shuo. Antibacterial Effect and Mechanism of Bacillus siamensis JZ 1-4-10 on Dry Rot Disease of Potato[J]. Chinese Journal of Biological Control, 2024, 40(3): 641-651.

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暹罗芽胞杆菌JZ1-4-10对马铃薯干腐病的抑菌作用及机理

Antibacterial Effect and Mechanism of Bacillus siamensis JZ 1-4-10 on Dry Rot Disease of Potato

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