新疆紫苜蓿叶象内共生菌Wolbachia的感染和分子鉴定

doi:10.16409/j.cnki.2095-039x.2024.03.008

摘要/Abstract

摘要： 紫苜蓿叶象Hypera postica Gyllenhal是为害新疆苜蓿生产的主要害虫。为明确新疆紫苜蓿叶象内共生菌Wolbachia感染的分布特征、感染成因和所属超群，本研究对新疆6地区20县市的紫苜蓿叶象进行样本采集，基于Wolbachia表面蛋白基因wsp和多位点序列分型系统（MLST）的5个基因（gatB、coxA、hcpA、ftsZ和fbpA）进行扩增和测序，对序列进行BLAST比对并构建系统发育树，对供试样本感染Wolbachia菌株进行分子鉴定。结果表明：在新疆20个县市供试样本中，仅在昌吉州和博州6个县市的紫苜蓿叶象种群中有Wolbachia感染，其中在呼图壁县的感染率（58%）最高，在阜康市感染率（6%）最低； Wolbachia感染率仅与样地经度呈显著正相关关系。单基因数据集ML树和多基因数据集贝叶斯树分析结果均表明，新疆紫苜蓿叶象种群感染的Wolbachia菌株wHypera4具有独特性，属于A超群，而不是先前已知的B超群，这为挖掘新的生物防治方法提供了理论依据。

关键词: 紫苜蓿叶象, Wolbachia, wsp基因, MLST, 系统发育分析

Abstract: The alfalfa weevil, Hypera postica Gyllenhal, is a major pest affecting alfalfa production in Xinjiang. To elucidate the distribution, causes of infection, and supergroup affiliation of Wolbachia endosymbionts in H. postica from Xinjiang, this study collected samples from 20 counties across six regions. Amplification and sequencing were performed for the Wolbachia surface protein gene (wsp) and five genes (gatB, coxA, hcpA, ftsZ, and fbpA) associated with the multilocus sequence typing (MLST) system. Sequence analysis, BLAST comparison, and phylogenetic tree construction were used to molecularly identify Wolbachia strains infecting the sampled H. postica populations. The results indicated that Wolbachia infections were detected only in H. postica populations from six counties within Changji and Bortala Prefectures, with the highest infection rate (58%) observed in Hutubi County and the lowest (6%) in Fukang City. Notably, the Wolbachia infection rate was significantly positively correlated with the longitude of the sampling sites. Both single-gene maximum likelihood (ML) trees and multi-gene Bayesian inference analyses revealed that the Wolbachia strain wHypera4, found in Xinjiang's H. postica, exhibits unique characteristics and belongs to supergroup A, rather than the previously known supergroup B. This finding provides a theoretical basis for exploring novel biological control strategies.

Key words: Hypera postica, Wolbachia, wsp gene, MLST, phylogenetic analysis

中图分类号:

S476

王阳, 刘晓晴, 任金龙, 赵莉. 新疆紫苜蓿叶象内共生菌Wolbachia的感染和分子鉴定[J]. 中国生物防治学报, 2024, 40(5): 1160-1168.

WANG Yang, LIU Xiaoqing, REN Jinlong, ZHAO Li. Infection and Molecular Characterization of Wolbachia in the Alfalfa Weevil (Coleoptera: Curculionidae) in Xinjiang[J]. Chinese Journal of Biological Control, 2024, 40(5): 1160-1168.

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