瓜类细菌性果斑病拮抗菌ZY1的鉴定及其生物学特性

doi:10.16409/j.cnki.2095-039x.2024.02.064

中国生物防治学报 ›› 2024, Vol. 40 ›› Issue (6): 1430-1438.DOI: 10.16409/j.cnki.2095-039x.2024.02.064

瓜类细菌性果斑病拮抗菌ZY1的鉴定及其生物学特性

杨玉文^1,3, 刘德华², 王成梁^1,4, 孟永红⁵, 关巍¹, 赵廷昌^1,3

1. 中国农业科学院植物保护研究所/植物病虫害综合治理全国重点实验室, 北京 100193;
2. 安徽江淮园艺种业股份有限公司, 合肥 230031;
3. 三亚中国农业科学院国家南繁研究院, 三亚 572024;
4. 吉林农业大学, 长春 130118;
5. 乐东都知果西密瓜种植农民专业合作社, 乐东 572541

出版日期:2024-12-08 发布日期:2024-12-12
通讯作者: 赵廷昌, 杨玉文
作者简介:杨玉文,男,助理研究员,E-mail:yangyuwen@126.com;通信作者,赵廷昌,研究员,E-mail:zhaotgcg@163.com;杨玉文,助理研究员,E-mail:yangyuwen@126.com。
基金资助:
海南省科技专项资助(ZDYF2023XDNY084);国家重点研发计划(2023YFD1401200);安徽省重点研发计划(2023j11020004);国家西甜瓜产业技术体系(CARS-25)

Identification and Biological Characteristics of Bacillus velezensis ZY1 against Bacterial Fruit Blotch

YANG Yuwen^1,3, LIU Dehua², WANG Chengliang^1,4, MENG Yonghong⁵, GUAN Wei¹, ZHAO Tingchang^1,3

1. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Anhui Jianghuai Horticulture Seed Industry Co., Ltd., Hefei 230031, China;
3. National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China;
4. Jilin Agricultural University, Changchun 130118, China;
5. Ledong Douzhiguo Melon and Watermelon Planting Professional Farmers' Cooperative, Ledong 572541, China

Online:2024-12-08 Published:2024-12-12

摘要/Abstract

摘要： 在辣椒叶片中分离到一株对瓜类细菌性果斑病菌具有显著拮抗作用的菌株ZY1。通过形态特征观察、Biolog测定及多基因系统发育分析，鉴定菌株ZY1为贝莱斯芽胞杆菌Bacillus velezensis。对菌株ZY1的生物学测定结果表明，菌株ZY1在pH 5～9和NaCl浓度2%～7%条件下均能够生长，25 ℃～40 ℃为最适生长温度。通过抑菌实验分析，拮抗菌ZY1对瓜类细菌性果斑病I组菌株pslb65和II组菌株Aac5均具有较强的抑菌作用。酶学试验结果表明，菌株ZY1能够产生纤维素酶、果胶酶、蛋白酶和淀粉酶等代谢物质。同时，该菌株还具有解磷和产氨能力。本文首次报道了一株对瓜类细菌性果斑病菌具有抗菌作用的贝莱斯芽胞杆菌ZY1，该菌为瓜类细菌性果斑病的生物防治以及土壤改良提供了新的微生物资源。

关键词: 贝莱斯芽胞杆菌, 鉴定, 抗逆性, 生物防治

Abstract: The strain ZY1 was isolated from pepper leaves, which has a significant antagonistic effect on bacterial fruit blotch. Based on morphological observation, Biolog analysis, and multi gene phylogenetic analysis, strain ZY1 was identified as Bacillus velezensis. To evaluate the stress resistance of strain ZY1, its growth status was studied under different pH, NaCl concentration, and temperature. The results showed that strain ZY1 growed rapidly and has strong stress resistance. It could grow normally under conditions of pH 5-9, NaCl concentration of 2%-7%, and 25 ℃-40 ℃, and the growth of the strain was significantly inhibited at 55 ℃. The antibacterial experiment results showed that strain ZY1 had strong antibacterial effects on both group I and group II strain Aacs. The enzymatic test results showed that strain ZY1 could produce metabolites such as cellulase, pectinase, protease, and amylase. At the same time, the strain also had the ability to hydrolyze phosphorus and produce ammonia. This article reported for the first time a strain of Bacillus velezensis, which had antibacterial activity against bacterial fruit blotch. This strain ZY1 provided a new microbial resource for the biological control and soil improvement.

Key words: Bacillus velezensis, identification, stress resistance, biological control

中图分类号:

S476

杨玉文, 刘德华, 王成梁, 孟永红, 关巍, 赵廷昌. 瓜类细菌性果斑病拮抗菌ZY1的鉴定及其生物学特性[J]. 中国生物防治学报, 2024, 40(6): 1430-1438.

YANG Yuwen, LIU Dehua, WANG Chengliang, MENG Yonghong, GUAN Wei, ZHAO Tingchang. Identification and Biological Characteristics of Bacillus velezensis ZY1 against Bacterial Fruit Blotch[J]. Chinese Journal of Biological Control, 2024, 40(6): 1430-1438.

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瓜类细菌性果斑病拮抗菌ZY1的鉴定及其生物学特性

Identification and Biological Characteristics of Bacillus velezensis ZY1 against Bacterial Fruit Blotch

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