Abstract: B946 (Bacillus cereus), a strain of the beneficial endophytic bacteria isolated from wheat, could prevent sharp eyespot of wheat. After it was marked with streptomycin and rifampin, colonization of the strain sprayed onto leaves in root, stem-base and leaf of wheat was investigated through diluting method. The results showed that the target strain could colonize internal leaf tissues inoculated by B946 and transfer to internal stem-base, the other leaves and root. And it could also transfer to stem-base and leaf of wheat when seeds were dipped. Higher concentration than 10⁸ cfu/ml, 3h for seed treatment, and plant-growing temperature at 25℃ were good for the colonization and transference.