烟嘧磺隆降解菌2N3的根部定殖及对黄瓜和油菜的修复效果

doi:10.16409/j.cnki.2095-039x.2026.02.041

摘要/Abstract

摘要： 烟嘧磺隆是玉米田常用除草剂，其土壤残留累积会抑制玉米幼苗生长并对后茬敏感植物产生药害。本研究旨在探究降解菌吉林克雷伯氏菌Klebsiella jilinsis 2N3对该污染土壤的修复效果及机制，以8 mg/kg烟嘧磺隆为胁迫条件，显著抑制了玉米幼苗的生长，茎叶干重降幅最高达75.11%。测土壤中添加2N3处理，28 d后对烟嘧磺隆降解率达66.02%。采用GFP标记法测定菌株2N3在根部定殖情况表明，接种（初始浓度1×10⁸ CFU/g）21 d后定殖量为8.51×10⁴ CFU/g。以油菜、黄瓜为敏感植物开展修复试验结果表明，与外源添加1 mg/kg烟嘧磺隆对照相比，添加菌株2N3处理后对两种蔬菜的生长有显著恢复作用，油菜株高、根长、茎叶鲜重增幅分别为26.70%、51.94%和34.89%，黄瓜株高、根长、茎叶鲜重增幅分别为13.95%、7.86%和9.96%；两种蔬菜叶片SOD、POD活性提升，MDA含量降低，TTC根系活力提升。本研究表明菌株2N3可有效降解土壤中的烟嘧磺隆并对油菜、黄瓜生长有修复作用。

关键词: 烟嘧磺隆, 降解菌2N3, 根际定殖, 敏感植物, 微生物修复

Abstract: As a commonly used herbicide in maize fields, the soil residue accumulation of nicosulfuron can inhibit maize seedling development and cause phytotoxicity to susceptible following plants. This study aims to investigate the remediation capacity and mechanism of the nicosulfuron-degrading bacterium Klebsiella jilinsis 2N3 on contaminated soil. Under the stress condition of 8 mg/kg nicosulfuron, the growth of maize seedlings was significantly inhibited, with the maximum reduction in stem and leaf dry weight reaching 75.11%. Inoculation of strain 2N3 achieved a 66.02% nicosulfuron degradation efficiency after 28 days of treatment. GFP labeling assays revealed that the root colonization population of strain 2N3 decreased from an initial density of 1×10⁸ CFU/g to 8.51×10⁴ CFU/g at 21 days post-inoculation. Pot bioassays using rapeseed and cucumber as susceptible plants revealed that strain 2N3 significantly restored the growth of both vegetables compared to the 1 mg/kg nicosulfuron control. Plant height, root length and shoot fresh weight of rapeseed increased by 26.70%, 51.94% and 34.89%, whereas those of cucumber increased by 13.95%, 7.86% and 9.96%, respectively. Furthermore, strain 2N3 enhanced leaf SOD and POD activities, lowered MDA levels, and promoted root activity in both vegetables. These findings verify that strain 2N3 is capable of degrading nicosulfuron in soil and mitigates herbicide phytotoxicity in sensitive vegetable crops.

Key words: nicosulfuron, degradation bacteria 2N3, rhizosphere colonization, susceptible plant, microbial bioremediation

中图分类号:

S476

刘雪微, 祝熙砚, 翟乾行, 代刚剑, 张崇慧, 刘海军, 张浩. 烟嘧磺隆降解菌2N3的根部定殖及对黄瓜和油菜的修复效果[J]. 中国生物防治学报, 2026, 42(4): 983-992.

LIU Xuewei, ZHU Xiyan, ZHAI Qianhang, DAI Gangjian, ZHANG Chonghui, LIU Haijun, ZHANG Hao. Root Colonization of Nicosulfuron-Degrading Strain 2N3 and Its Remediation Effect on Cucumber and Rape[J]. Chinese Journal of Biological Control, 2026, 42(4): 983-992.

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