Abstract: Twenty two strains (14 bacteria and 8 actinomyces strains) of antagonistic microorganisms against Fulvia fulva were isolated from vegetable field soil. Results showed that spore germination of F. fulva inhibited by fermented liquid and the supernatant of bacteria X₁₄ and actinomyces F₁₅ completely, the average inhibitory distance of strain X₁₄ against mycelial growth reached to 22.2 mm, and its control effect on leaf mould of tomato was between 79.8% and 89.0%. According to the morphological feature, X₁₄ was identified as Bacillus. The optimum carbohydrates nutrition, nitride nutrition and pH value was amylum, NH₄NO₃ and 6.0, respectively for X₁₄ fermentation.