运用电激转化技术构建杀虫工程菌

运用电激转化技术构建杀虫工程菌

张光焰; 张杰; 彭于发; 陈彩层; 黄大昉; 赵建周

1.中国农科院植保所植物病虫害生物学国家重点实验室,北京 100094;2.中国农科院蔬菜花卉所,北京 100081

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1993-11-08 发布日期:1993-11-08

CONSTRUCTION OF GENETICALLY ENGINEERED BACTERIA AGAINST INSECT PESTS WITH ELECTROPORATION

ZHANG Guang-yan¹;ZHANG Jie¹;PENG Yu-fa¹;CHEN Cai-ceng¹;HUANG Da-fang¹; ZHAO Jian-zhou²

1.The National Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100094;2.Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijng 100081

Received:1900-01-01 Revised:1900-01-01 Online:1993-11-08 Published:1993-11-08

摘要/Abstract

摘要： <正> 将苏云金杆菌(Bacillus thuringiensis)内毒素杀虫蛋白基因(简称Bt基因)导入荧光假单胞菌(Pseudomonas fluorescens),创建新型微生物农药,在国外已有产品试销。在国内,也已引起广泛重视。本实验室曾运用转座子接合转移技术,将Bt基因导入对小麦全蚀病防治效果显著、并有促进植物生长作用的荧光假单胞菌P303菌株中,构建了兼有杀虫防病作用的工程菌。此后,又运用电激转化技术将带有Bt基因的重组质粒转入P303菌株中,获得了具有杀虫活性的PT410、PT415等工程菌株。

张光焰; 张杰; 彭于发; 陈彩层; 黄大昉; 赵建周. 运用电激转化技术构建杀虫工程菌[J]. , DOI: .

ZHANG Guang-yan¹;ZHANG Jie¹;PENG Yu-fa¹;CHEN Cai-ceng¹;HUANG Da-fang¹; ZHAO Jian-zhou². CONSTRUCTION OF GENETICALLY ENGINEERED BACTERIA AGAINST INSECT PESTS WITH ELECTROPORATION[J]. , DOI: .