家蚕灰僵病种群遗传结构研究

doi:10.16409/j.cnki.2095-039x.2016.01.009

中国生物防治学报 ›› 2016, Vol. 32 ›› Issue (1): 55-61.DOI: 10.16409/j.cnki.2095-039x.2016.01.009

家蚕灰僵病种群遗传结构研究

张胜利^1,2, 陈雪², 蒲顺昌¹, 徐延平², 何灵敏², 李增智², 栾丰刚³

1. 亳州师范高等专科学校生物工程研究所, 亳州 236800;
2. 安徽农业大学微生物防治省重点实验室, 合肥 230036;
3. 江西农业大学林学院, 南昌 330045

收稿日期:2015-03-23 出版日期:2016-02-08 发布日期:2016-02-22
通讯作者: 栾丰刚
作者简介:张胜利(1984-),男,工程师,E-mail:victory.z@163.com
基金资助:
安徽省高校自然科学基金(KJ2013B152);金蝉花产品开发创新研究团队(校教字[2014]60);江西省科技计划项目(20132BAB204024)

Population Genetic Structure Analysis of Insect Fungal Pathogen Causing Silkworm Grey Muscardine

ZHANG ShengLi^1,2, CHEN Xue², PU Shunchang¹, XU Yanping², HE Lingmin², LI Zengzhi², LUAN Fenggang³

1. Institute of Biological Engineering, Bozhou Teachers College, Bozhou 236800, China;
2. Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036, China;
3. College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China

Received:2015-03-23 Online:2016-02-08 Published:2016-02-22

摘要/Abstract

摘要： 家蚕灰僵病为低频率发生的家蚕真菌性地方病,病原为爪哇棒束孢。采用ISSR分子标记对安徽潜山和浙江桐乡的2个家蚕灰僵病病原种群进行了指纹图谱分析。利用12个ISSR引物共扩增出132个位点,其中多态位点数为93个,占70.45%。在种群水平上的Nei's遗传多样性指数(h)和Shannon's指数(Is)具有一致性。采用UPGMA法进行聚类分析,来自2个地区的19个菌株在遗传相似系数0.76处完全按地理来源分为2个类群;基于ISSR信息的三维主坐标分析(PCA)结果与分子聚类结果一致。2个家蚕灰僵病病原种群间的基因流(Nm)为0.5106,遗传分化系数(Gst)为0.3287,说明种群间遗传多样性高于种群内。2个种群单独聚类表明,潜山地方病种群在0.8的相似处有一个明显的优势类群,而桐乡种群未表现出优势性。上述分析可知,家蚕灰僵病病原种群表现出与地理来源相关的空间异质性,种群异质性是地方病种群维持的重要原因。

关键词: 家蚕, 灰僵病, 爪哇棒束孢, 地方病, ISSR

Abstract: Fungal pathogen Isaria javanica can cause a low rate of silkworm grey muscardine. In this study, two I. javanica populations collected from Qianshan and Tongxiang were fingerprinting analyzed using ISSR markers. In the assayed 19 isolates, 120 discernible DNA bands ranging in size from 200 to 2000 bp were generated by 12 ISSR primers, and 93 of the bands (70.45%) were polymorphic. Nei's gene diversity (h) and Shannon's Information index (I_s) showed a similar trend with the percentage of polymorphism at the population level. At the point of genetic similarity coefficient 0.76, the strains could be divided into 2 groups which correlated with the geographic origin of sampling. The result of 3-D principal coordinate analyses (PCA) based on ISSR data shared high similarity with UPGMA cluster analysis. The gene flow (N_m) between the two populations was 0.5678, and the genetic differentiation coefficient (G_st) was 0.3287, which indicates higher genetic diversity between populations than within populations. Single population clustering results showed that Qianshan population had an evident dominant group at the genetic similarity coefficient of 0.8, while Tongxiang population did not have a predominant lineage. Based on the analyses above, we can conclude that pathogen populations causing enzootic grey muscardine are geographically heterogenous, which may be an important factor for the maintenance of enzootic populations.

Key words: Bombyx mori, grey muscardine, Isaria javanica, enzootic, ISSR

中图分类号:

S476.12

张胜利, 陈雪, 蒲顺昌, 徐延平, 何灵敏, 李增智, 栾丰刚. 家蚕灰僵病种群遗传结构研究[J]. 中国生物防治学报, 2016, 32(1): 55-61.

ZHANG ShengLi, CHEN Xue, PU Shunchang, XU Yanping, HE Lingmin, LI Zengzhi, LUAN Fenggang. Population Genetic Structure Analysis of Insect Fungal Pathogen Causing Silkworm Grey Muscardine[J]. journal1, 2016, 32(1): 55-61.

参考文献

[1] 孙黎峰, 韩益飞, 杨佩华, 等. 2008年江苏省秋季蚕病疫情调查分析[J]. 江苏蚕业, 2009, (3):7-11
[2] 李佳丽, 蔡悦, 栾丰刚, 等. 皖南与皖西南蚕区球孢白僵菌种群遗传结构的ISSR分析[J]. 应用生态学报, 2010, 21(12):3239-3247.
[3] 穆莉. 家蚕的真菌病//中国虫生真菌研究与应用(第三卷)[M]. 北京:中国农业科学技术出版社, 1993, 50-53.
[4] 朱方容, 邱全立, 何彬, 等. 寄生桑粉虱的拟青霉菌对家蚕的致病性研究[J]. 蚕业科学, 1997, 22(3):137-141.
[5] 黄勃, 王四宝, 金伟, 等. 家蚕灰僵病病原的鉴定[J]. 蚕业科学, 2008, 34(2):257-261.
[6] 朱衡, 瞿峰, 朱立煌. 利用氯化苄提取适于分子生物学分析的真菌DNA[J]. 菌物系统, 1994, 13(1):34-40.
[7] Wang S, Miao X, Zhao W, et al. Genetic diversity and population structure among strains of the entomopathogenic fungus, Beauveria bassiana, as revealed by inter-simple sequence repeats (ISSR)[J]. Mycological Research, 2005, 109(12):1364-1372.
[8] Hall T A. BioEdit:a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT[J]. Nucleic Acids Symposium Series, 1999, 41:95-98.
[9] Tamura K, Dudley J, Nei M, et al. MEGA4:Molecular evolutionary genetics analysis (MEGA) software version 4.0[J]. Molecular Biology and Evolution, 2007, 24(8):1596-1599.
[10] 王勇, 陈克平, 姚勤. 系统发生分析软件PAUP和TreePuzzle使用方法介绍[J]. 安徽农业科学, 2008, 36(2):818-821, 829.
[11] Swofford D L. PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4[M]. Massachusetts:Sinauer Associates, 2003.
[12] Felsenstein J. Confidence limits on phylogenies:an approach using the bootstrap[J]. Evolution, 1985, 39(4):783-791.
[13] Ronquist F, Huelsenbeck J P. MrBayes 3:Bayesian phylogenetic inference under mixed models[J]. Bioinformatics, 2003, 19(12):1572-1574.
[14] 王勇, 陈克平, 姚勤. 系统发生分析程序MrBayes 3.1使用方法介绍[J]. 安徽农业科学, 2009, 37(33):16665-16669.
[15] Yeh F C, Yang R C, Boyle T B J, et al. POPGENE, the user-friendly shareware for population genetic analysis//Molecular Biology and Biotechnology Centre[M]. University of Alberta, 1997.
[16] Rohlf F J. NTSYS-pc:Numerical Taxonomy and Multivariate Analysis System, Version 2.1, User Guide[M]. New York:Exeter Software, 2000.
[17] 朱方容. 广西部分地区桑蚕僵病流行的原因及防控对策[J]. 广西蚕业, 2008, 45(3):29-32.
[18] 孙始威, 孙振兴, 葛宜和, 等. 基于ISSR标记的扁玉螺(Neverita didyma)自然居群遗传结构[J]. 生态学报, 2008, 28(11):5499-5505.
[19] 杜广祖, 王晶晶, 陈斌. 云南球孢白僵菌的遗传多样性研究[J]. 云南农业大学学报, 2012, 27(3):327-333.
[20] 栾丰刚. 引起昆虫自然流行病的昆虫病原真菌种群遗传结构研究[D]. 合肥:安徽农业大学, 2011.
[21] Tigano-Milani M S, Honeycutt R J, Lacey L A, et al. Genetic variability of Paecilomyces fumosoroseus isolates revealed by molecular markers[J]. Journal of Invertebrate Pathology, 1995, 65(3):274-282.
[22] Estrada M E, Camacho M V, Benito C. The molecular diversity of different isolates of Beauveria bassiana (Bals.) Vuill. as assessed using intermicrosatellites (ISSRs)[J]. Cellular and Molecular Biology Letters, 2007, 12(2):240-252.
[23] Glare T R, Inwood A J. Morphological and genetic characterization of Beuvaria spp. from New Zealand[J]. Mycological Research, 1998, 102(2):250-256.
[24] Fernandes E K K, Moraes A M L, Pacheco R S, et al. Genetic diversity among Brazilian isolates of Beauveria bassiana:comparisons with non-Brazilian isolates and other Beauveria species[J]. Journal of Applied Microbiology, 2009, 107(3):760-774.
[25] 胡晓磊, 何玲敏, 陈雪, 等. 中国南方球孢白僵菌的遗传多样性和种群遗传结构[J]. 中国生物防治学报, 2013, 29(1):31-41.
[26] 何玲敏, 胡晓磊, 陈雪, 等. 中国北方球孢白僵菌的遗传多样性和种群遗传结构[J]. 应用生态学报, 2012, 23(11):3087-3095.
[27] Shimazu M, Takatsuka J. Isaria javanica (anamorphic Cordycipitaceae) isolated from gypsy moth larvae, Lymantria dispar (Lepidoptera:Lymantriidae), in Japan[J]. Applied Entomology and Zoology, 2010, 45(3):497-504.
[28] Specht A, Azevedo J L, de Luna E A, et al. Occurrence of the entomopathogenic fungus Isaria javanica (Frieder. & Bally) Samson & Hywell-Jones (Fungi, Sordariomycetes) infecting Lonomia obliqua Walker (Lepidoptera, Saturniidae, Hemileucinae) caterpillars[J]. Revista Brasileira De Entomologia, 2009, 53(3):493-494.
[29] Scorsetti A C, Humber R A, de Gregorio C, et al. New records of entomopathogenic fungi infecting Bemisia tabaci and Trialeurodes vaporariorum, pests of horticultural crops, in Argentina[J]. BioControl, 2008, 53(5):787-796.
[30] 展茂魁, 何玲敏, 陈名君, 等. 防治假眼小绿叶蝉的虫生真菌高毒菌株的筛选及田间防治效果[J]. 中国生物防治学报, 2012, 28(1):41-46.

家蚕灰僵病种群遗传结构研究

Population Genetic Structure Analysis of Insect Fungal Pathogen Causing Silkworm Grey Muscardine

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

本文评价

联系我们

[1]	黄翠, 王品, 陈雪, 杨欣, 张大敏, 李增智. 球孢白僵菌松毛虫分离株对家蚕的致病力[J]. , 2014, 30(2): 199-209.
[2]	陈名, 张大敏, 彭凡, 李增智. 用于防治假眼小绿叶蝉的爪哇棒束孢可湿性粉剂的研制[J]. , 2014, 30(1): 51-57.
[3]	胡晓磊, 何玲敏, 陈雪, 张胜利, 栾丰刚, 李增智. 中国南方球孢白僵菌的遗传多样性和种群遗传结构[J]. , 2013, 29(1): 31-41.
[4]	李杨, 李会, 尹淑艳, 尹真, 孙绪艮. 日本追寄蝇生物学特性研究[J]. , 2012, 28(1): 27-31.
[5]	展茂魁, 何玲敏, 陈名, 王滨, 李增智. 防治假眼小绿叶蝉的虫生真菌高毒菌株的筛选及田间防治效果[J]. , 2012, 28(1): 41-46.
[6]	邵宗泽;喻子牛. 粘虫幼虫对苏云金芽孢杆菌伴孢晶体低度敏感的机理探讨[J]. , 1999, 15(2): 66-69.
[7]	邵宗泽;崔云龙. 两种鳞翅目幼虫对Bt敏感性的差异及其机理的探讨 [J]. , 1995, 11(2): 75-79.