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中国生物防治学报 ›› 2016, Vol. 32 ›› Issue (1): 101-105.DOI: 10.16409/j.cnki.2095-039x.2016.01.015

• 研究论文 • 上一篇    下一篇

海滨斯氏线虫生物学特性研究

刘瑞, 刘奇志   

  1. 中国农业大学植物保护学院, 北京 100193
  • 收稿日期:2015-03-11 出版日期:2016-02-08 发布日期:2016-02-22
  • 通讯作者: 刘奇志
  • 作者简介:刘瑞(1990-),女,硕士研究生,E-mail:liurui900920@163.com
  • 基金资助:
    国家公益性行业(林业)科研专项(201004037);国家科技支撑计划(2014BAD20B01)

Study on the Biological Characteristics of Steinernema litorale

LIU Rui, LIU Qizhi   

  1. College of Plant Protection, China Agricultural University, Beijing 100193, China
  • Received:2015-03-11 Online:2016-02-08 Published:2016-02-22

摘要: 研究旨在明确采自北京延庆县杏树根际土壤中海滨斯氏线虫Steinernema litorale的生物学特性,进而明确开发应用的潜能。结果表明:在侵染24 h、剂量640 IJs/幼虫下,大蜡螟校正死亡率达76.7%;48 h时,除10和20 IJs/幼虫外,40、80、160、320和640 IJs/幼虫剂量下大蜡螟的校正死亡率均为100.0%。在侵染36 h,剂量160、320和640 IJs/幼虫下,黄粉虫校正死亡率均达50.0%以上;48 h时,剂量640 IJs/幼虫下,黄粉虫校正死亡率为100.0%。经过50.5% RH下干燥9 h后,海滨斯氏线虫存活率仍达94.0%。线虫在30℃条件下的水平运动能力强于18和25℃下,24 h后有62.7%爬行3.6 cm寻找到寄主体液。水浴6 h时,30、32、34、35℃下线虫的死亡率均在50.0%以上;38℃水浴2 h后,线虫的死亡率为100.0%。研究结果表明,海滨斯氏线虫具有很高的侵染力和长时间的耐干燥力,显示了其防治害虫的开发利用潜能。

关键词: 海滨斯氏线虫, 侵染力, 耐干燥, 耐热性, 水平运动能力

Abstract: The biology analysis on Steinernema litorale, isolated from the apricot tree soil in Yanqing district of Beijing, was studied to understand the potential of being bio-control agent. The results showed that when larvae were exposed to 640 infective juveniles (IJs)/larva for 24 h, 76.7% mortality of Galleria mellonella were recorded. The corrected mortality of G. mellonella was 100.0% at dosages of 40, 80, 160, 320, 640 IJs/larva, except of the dosages of 10 and 20 IJs/larva after 48 h. The corrected mortality of Tenebrio molitor was more than 50% at the dosages of 160, 320 and 640 IJs/larva after 36 h, and 100.0% at the dosages of 640 IJs/larva after 48 h. The survival rate of S. litorale was still up to 94.0% at 50.5% RH desiccated for 9 h. S. litorale ability of transverse diffusion at 30℃ was better than that at 18 and 25℃. There was 62.7% individuals could move 3.6 cm in 24 h to reach the host at 30℃. More than 50.0% mortality rate of S. litorale was obtained in water bath at 30, 32, 34 and 35℃ for 6 h, 100.0% at 38℃ for 2 h. According to the results, S. litorale has high infectivity and long resistance desiccation capacity, which shown its potential of development and application to control pest.

Key words: Steinernema litorale, infection, desiccation tolerance, heat resistance, transverse diffusion

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