黄瓜PGPR菌株S160的鉴定及其对重金属铅的吸附研究

doi:10.16409/j.cnki.2095-039x.2016.01.020

中国生物防治学报 ›› 2016, Vol. 32 ›› Issue (1): 129-134.DOI: 10.16409/j.cnki.2095-039x.2016.01.020

• 研究简报 • 上一篇

黄瓜PGPR菌株S160的鉴定及其对重金属铅的吸附研究

秦士娇¹, 李娟¹, 王远宏¹, 康芬芬², 刘可¹, 樊乐¹, 常若葵³

1. 天津农学院园艺园林学院, 天津 300384;
2. 天津出入境检验检疫局, 天津 300461;
3. 天津农学院工程技术学院, 天津 300384

收稿日期:2015-03-18 出版日期:2016-02-08 发布日期:2016-02-22
通讯作者: 王远宏, 常若葵
作者简介:秦士娇,女,大学生,E-mail:qinsj1003@163.com
基金资助:
国家自然科学基金(31171892);大学生创新训练项目(201410061044)

Identification of PGPR Strain S160 on Cucumber and Its Adsorption for Lead Ions

QIN Shijiao¹, LI Juan¹, WANG Yuanhong¹, KANG Fenfen², LIU Ke¹, FAN Le¹, CHANG Ruokui³

1. College of Horticulture, Tianjin Agriculture University, Tianjin 300384, China;
2. Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin 300461, China;
3. College of Engineering and Technology, Tianjin Agriculture University, Tianjin 300384, China

Received:2015-03-18 Online:2016-02-08 Published:2016-02-22

摘要/Abstract

摘要： 为探索植物根际促生菌对枯萎病的防效及其对重金属吸附能力,从黄瓜根际土壤中筛选得到一株细菌S160。根据菌株形态、生理生化特性,并结合16S rDNA序列分析,鉴定为粘质沙雷氏菌Serratiamarcescens。盆栽苗沾根接种菌株S160分析其防效和促生定殖能力,结果表明菌株S160对黄瓜苗有显著的促生作用,根部定殖量达到7.20×10³ CFU/g,对枯萎病的防效可达57.2%。利用原子吸收测定S160菌液中Pb²⁺的浓度变化,菌株S160可以吸附培养基中的Pb²⁺,吸附率可达47.63%。研究结果揭示了菌株S160良好的防病促生及其修复重金属铅污染的潜力。

关键词: PGPR, 粘质沙雷氏菌, 黄瓜枯萎病菌, Pb²⁺

Abstract: A bacterial isolate S160 from cucumber rhziosphere was obtained and screened for its ability to promote cucumber growth, control cucumber Fusarium oxysporum and absorb heavy metal Pb²⁺. Based on comparative analysis of 16S rDNA sequence, morphology and chemotaxonomic traits, strain S160 was identified as Serratia marcescens. By pot experiment, control efficiency against cucumber F. oxysporum was evaluated with 57.2%. Growth promotion assay in cucumber showed better promoting effect and root colonization capacity, with colonization quantity is about 7.20×103 CFU/g. Atomic absorption spectroscopy was used to measure the concentration of Pb²⁺ in the liquid medium after treated with strain S160. The concentration of Pb²⁺ decreased after treated with strain S160 and the adsorption rate of Pb²⁺ reached 47.63%. These results indicate that strain S160 share a certain capacity in controlling cucumber Fusarium wilt, promoting cucumber growth and remediation of heavy metal Pb²⁺.

Key words: PGPR, Serratia marcescens, Fusarium oxysporum, Pb²⁺

中图分类号:

S476

秦士娇, 李娟, 王远宏, 康芬芬, 刘可, 樊乐, 常若葵. 黄瓜PGPR菌株S160的鉴定及其对重金属铅的吸附研究[J]. 中国生物防治学报, 2016, 32(1): 129-134.

QIN Shijiao, LI Juan, WANG Yuanhong, KANG Fenfen, LIU Ke, FAN Le, CHANG Ruokui. Identification of PGPR Strain S160 on Cucumber and Its Adsorption for Lead Ions[J]. journal1, 2016, 32(1): 129-134.

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黄瓜PGPR菌株S160的鉴定及其对重金属铅的吸附研究

Identification of PGPR Strain S160 on Cucumber and Its Adsorption for Lead Ions

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