红颈常室茧蜂雌性生殖系统及卵巢管形态学观察

doi:10.16409/j.cnki.2095-039x.2019.04.011

中国生物防治学报 ›› 2019, Vol. 35 ›› Issue (4): 517-526.DOI: 10.16409/j.cnki.2095-039x.2019.04.011

红颈常室茧蜂雌性生殖系统及卵巢管形态学观察

夏亚运^1,2, 田苗苗^1,2, 李明明³, 毛建军¹, 蔡笃程², 罗淑萍¹

1. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193;
2. 海南大学热带农林学院, 海口 570228;
3. 湖南农业大学植物保护学院, 长沙 410128

收稿日期:2018-12-20 出版日期:2019-08-08 发布日期:2019-08-10
通讯作者: 罗淑萍,副研究员,E-mail:shp.l@126.com。
作者简介:夏亚运,硕士,E-mail:miaomiaotian1993@126.com
基金资助:
国家重点研发计划（2017YFD0201000）

Morphological Observation of the Female Reproductive System and Ovariole of Peristenus spretus (Hymenoptera: Braconidae)

XIA Yayun^1,2, TIAN Miaomiao^1,2, LI Mingming³, MAO Jianjun¹, CAI Ducheng², LUO Shuping¹

1. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricutural Sciences, Beijing 100193, China;
2. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China;
3. College of Plant Protection, Hunan Agricultural Univesity, Changsha 410128, China

Received:2018-12-20 Online:2019-08-08 Published:2019-08-10

摘要/Abstract

摘要： 利用光学显微镜和透射电镜观察红颈常室茧蜂Peristenus spretus Chen et van Achterberg雌性生殖系统结构、卵巢发育和卵子发生、卵子超微结构，并对15℃和23℃饲养条件下不同日龄的雌蜂卵巢管长度、卵巢管内卵室大小与成熟度进行了比较，揭示了该茧蜂雌性生殖系统结构与特征。结果表明：1）红颈常室茧蜂生殖系统结构主要包括一对卵巢、2条输卵管、中输卵管等；2）雌成虫在羽化前期（1～3 d）卵巢内的成熟卵粒较少，卵巢管内含有大量的卵原细胞，卵子内充满了大量的脂滴，中后期（5～11 d）随着脂肪体的降解吸收，卵子内逐渐新生一些线粒体、高尔基体等细胞器，其内存在滋养细胞、卵母细胞，并且滋养细胞无细胞膜存在。3）同一温度不同日龄红颈常室茧蜂卵粒数、成熟卵子数均差异显著，23℃下红颈常室茧蜂每条卵巢管内的日均卵子数20.70粒，显著高于15℃的日均卵子数18.65粒。

关键词: 红颈常室茧蜂, 卵巢发育, 卵巢管

Abstract: The structure of female reproductive system, ovarian development, oogenesis, egg ultrastructure, and ovariole growth under different temperatures in Peristenus spretus Chen et van Achterberg were observed using optical microscopy and transmission electron microscopy. The results showed that:1) The reproductive system of P. spretus consisted of a pair of ovaries, two oviducts and a median oviduct. 2) In the newly emerged females (1-3 d), the ovary had fewer mature eggs and the ovarioles contained a large number of oogonia cells filled with rich lipid droplets. In middle-aged femals (5-11 d), mitochondria and Golgi gradually emerged with the degradation and absorption of fat bodies. These organelles contained oocyte and nurse cells without cell membrane. 3) The number of matured oocytes and eggs in different ages was significantly different, the total number of eggs in each ovariole was 20.7 at 23℃, significantly more than that (18.65) at 15℃.

Key words: Peristenus spretus, ovarian development, ovarioles

中图分类号:

S476.3

夏亚运, 田苗苗, 李明明, 毛建军, 蔡笃程, 罗淑萍. 红颈常室茧蜂雌性生殖系统及卵巢管形态学观察[J]. 中国生物防治学报, 2019, 35(4): 517-526.

XIA Yayun, TIAN Miaomiao, LI Mingming, MAO Jianjun, CAI Ducheng, LUO Shuping. Morphological Observation of the Female Reproductive System and Ovariole of Peristenus spretus (Hymenoptera: Braconidae)[J]. Chinese Journal Of Biological Control, 2019, 35(4): 517-526.

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红颈常室茧蜂雌性生殖系统及卵巢管形态学观察

Morphological Observation of the Female Reproductive System and Ovariole of Peristenus spretus (Hymenoptera: Braconidae)

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