三种紫外线辐照对黑腹果蝇蛹的影响

doi:10.16409/j.cnki.2095-039x.2021.01.016

摘要/Abstract

摘要： 分别利用三种紫外灯（UVA、UVB、UVC）辐照黑腹果蝇蛹，研究辐照对黑腹果蝇的羽化率、性比、飞出率、死蛹率、成虫干重、蛹重以及F1羽化率、F1性比、F1死蛹率、F1成虫干重的影响。结果表明：辐照后黑腹果蝇羽化率均显著降低，其中UVB辐照6 h后的黑腹果蝇蛹羽化率最低，为10.00%；经过UVA辐照9 h组的性比平均值为2.32，显著高于其他处理组；经过UVB辐照后，黑腹果蝇飞出率显著降低，其中UVB处理6 h后黑腹果蝇飞出率低至4.00%；经过UVB辐照的死蛹率显著高于对照组和其他组，其中辐照6 h组黑腹果蝇蛹的死亡率平均值达到89.33%，最高达94.00%。经过紫外线辐照后各组蛹重与对照组相比都有不同程度的增加，且差异显著；经过UVB、UVC辐照后羽化的黑腹果蝇成虫干重显著低于对照组；经过紫外线辐照后黑腹果蝇F1成虫干重相比对照组均有所降低，与对照组差异显著。本研究可为昆虫的紫外线诱变和害虫生物防治提供参考。

关键词: 黑腹果蝇, 紫外线, 辐照

Abstract: The pupae of Drosophila melanogaster were irradiated by three kinds of ultraviolet lamps (UVA, UVB and UVC), and the effects of irradiation on the eclosion rate, sex ratio, flight rate, dead pupa rate, adult dry weight, pupa weight, F1 eclosion rate, F1 sex ratio, F1 dead pupa rate and F1 adult dry weight were studied. The results showed that the pupa emergence rate of D. melanogaster was significantly reduced after irradiation, among which the pupa emergence rate of D. melanogaster exposed to UVB for 6 h was the lowest (10.00%). The average sex ratio of 9 h group after UVA irradiation was 2.32, which was significantly higher than that of other treatment groups. After UVB irradiation, the pupa emergence rate of D. melanogaster decreased significantly, among which the pupa emergence rate of D. melanogaster was as low as 4.00% after 6 h of UVB treatment. The death rate of pupae in the group exposed to UVB was significantly higher than that in the control group and other groups, and the average death rate of pupae in the group exposed to UVB for 6 h reached 89.33% and the highest was 94.00%. After UV irradiation, pupa weight of each group increased significantly compared with that of the control group. After UVB and UVC irradiation, the dry weight of adult D. melanogaster was significantly lower than that of the control group. After UV irradiation, the dry weight of D. melanogaster F1 adult decreased compared with the control group, and the difference was significant. This study can provide reference for UV mutagenesis and biological control of insect pests.

Key words: Drosophila melanogaster, ultraviolet, irradiation

中图分类号:

S433

刘旭祥, 仪传冬, 敖国富, 陈湜, 季清娥. 三种紫外线辐照对黑腹果蝇蛹的影响[J]. 中国生物防治学报, 2021, 37(2): 228-234.

LIU Xuxiang, YI Chuandong, AO Guofu, CHEN Shi, JI Qinge. Effects of Three Kinds of Ultraviolet Radiation on Pupae of Drosophila melanogaster[J]. Chinese Journal of Biological Control, 2021, 37(2): 228-234.

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