斑鞘豆叶甲成虫对非寄主植物挥发物的触角电位和行为反应

doi:10.16409/j.cnki.2095-039x.2021.03.001

摘要/Abstract

摘要： 为筛选对斑鞘豆叶甲Colposcelis signata（Motschulsky）成虫有生物活性的行为调控剂，选取非寄主植物16种挥发物，采用触角电位技术（Electroantennogram，EAG）和Y型嗅觉仪测定了斑鞘豆叶甲成虫对挥发物的触角电位及行为反应。结果表明：10^-1mol/L浓度下斑鞘豆叶甲成虫对6种萜烯类挥发物EAG反应较强，驱避反应显著，雄虫反应强于雌虫。成虫对（1R）-（+）-α-蒎烯和（-）-β-蒎烯的EAG反应显著强于其他4种萜烯类挥发物，对雄虫的驱避率分别为86.7%和81.3%。斑鞘豆叶甲对5种醇、醛类挥发物EAG反应差异显著，除芳樟醇外均表现引诱反应。雌虫对反-2-己烯-1-醇、2-乙基-1-己醇和反-2-己烯醛3种绿叶气味挥发物的引诱反应强于雄虫，其中反-2-己烯-1-醇的引诱作用最强，引诱率达68.9%，推测雌虫产卵定位与绿叶气味挥发物有关。斑鞘豆叶甲对5种其他挥发物的EAG反应相对值较低，具有引诱作用，雄虫反应均强于雌虫，雌、雄虫对水杨酸甲酯的反应强于其他4种挥发物。浓度反应结果发现当浓度从10^-2 mol/L增加到10^-1mol/L时，成虫对（1R）-（+）-α-蒎烯和（-）-β-蒎烯的EAG反应相对值稍降低，对（-）-β-蒎烯和β-月桂烯的驱避反应降低，表现出浓度阈值。但在10^-5 mol/L浓度下（1R）-（+）-α-蒎烯、（-）-β-蒎烯、（R）-（+）-柠檬烯和β-月桂烯仍然表现为明显的驱避作用，这些挥发物可作为驱避剂组分加以开发利用。

关键词: 斑鞘豆叶甲, 非寄主植物挥发物, 触角电位, 行为反应

Abstract: The antennal and behavioral responses of Colposcelis signata adults to sixteen volatile compounds from non-host plants were measured using electroantennogram (EAG) and Y tube olfactometer to explore for behavioral manipulation agents for C. signata adults. The results showed that C. signata adults had strong EAG responses and obvious repellent responses to 6 terpenes volatile compounds at concentrations of 10^-1mol/L, the responses being stronger in males than females. The EAG responses to (1R)-(+)-α-pinene and (-)-β-pinene were significantly stronger than those to the other four terpenes, and the two compounds elicited male repellent rates of 86.7% and 81.3%, respectively. Colposcelis signata showed significantly different EAG responses to the five alcohols and aldehydes volatiles, which were all attractive responses except for the response to linalool. Compared to the males, the females exhibited significantly stronger attractive responses to the three green leaf volatiles, trans-2-hexen-1-ol, 2-ethyl-1-hexanol, and trans-2-hexenal. The attractive rate of trans-2-hexen-1-ol to the females was the highest, 68.9%. These green leaf volatiles may be related to the location of oviposition sites in this insect. Adults showed relatively low EAG responses and attractive responses to 5 other volatiles, but stronger in males than in females. Both females and males showed higher EAG responses and attractive responses to methyl salicylate than to the other 4 volatiles. The concentration response results indicated that, when the concentration increased from 10^-2mol/L to 10^-1mol/L, the EAG responses of adults to (1R)-(+)-α-pinene and (-)-β-pinene decreased slightly, and the repellent responses of adults to (-)-β-pinene and β-myrcene decreased and showed a concentration threshold. However, (1R)-(+)-α-pinene, (-)-β-pinene, (R)-(+)-limonene and β-myrcene even exhibited obvious repellent effects at a concentration of 10^-5mol/L. Therefore, these volatiles can be exploited as repellent components.

Key words: Colposcelis signata, non-host plant volatiles, electroantennogram (EAG), behavioral response

中图分类号:

S476

樊瑞冬, 张益恺, 毕嘉瑞, 徐伟, 臧连生. 斑鞘豆叶甲成虫对非寄主植物挥发物的触角电位和行为反应[J]. 中国生物防治学报, 2021, 37(4): 740-748.

FAN Ruidong, ZHANG Yikai, BI Jiarui, XU Wei, ZANG Liansheng. Electroantennogram and Behavioral Responses of Colposcelis signata Adults to Non-host Plant Volatiles[J]. Chinese Journal of Biological Control, 2021, 37(4): 740-748.

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