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中国生物防治学报 ›› 2022, Vol. 38 ›› Issue (5): 1075-1082.DOI: 10.16409/j.cnki.2095-039x.2022.09.003

• 转基因生物安全专栏 • 上一篇    下一篇

RNAi沉默HvFTZ-F1对茄二十八星瓢虫幼虫存活及发育的影响

王亚洁1, 刘卓琦1, 李华丽2, 潘慧鹏1, 杨春晓2   

  1. 1. 华南农业大学植物保护学院/广东省生物农药创制与应用重点实验室, 广州 510642;
    2. 华南农业大学亚热带农业生物资源保护与利用国家重点实验室, 广州 510642
  • 收稿日期:2022-07-13 发布日期:2022-10-20
  • 通讯作者: 杨春晓,博士,副研究员,E-mail:yangchunxiao@scau.edu.cn
  • 作者简介:王亚洁,硕士研究生,E-mail:wang-yajie@foxmail.com
  • 基金资助:
    国家自然科学基金(32272628,31972269)

The Effects of Silencing of HvFTZ-F1 via RNAi on the Survival and Development of the 28-Spotted Potato Ladybird,Henosepilachna vigintioctopunctata Larvae

WANG Yajie1, LIU Zhuoqi1, LI Huali2, PAN Huipeng1, YANG Chunxiao2   

  1. 1. College of Plant Protection, South China Agricultural University/Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, Guangzhou 510642, China;
    2. South China Agricultural University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangzhou 510642, China
  • Received:2022-07-13 Published:2022-10-20

摘要: 茄二十八星瓢虫Henosepilachna vigintioctopunctata是茄科植物上的一种重要害虫,对我国的作物生产造成了严重的经济损失。RNAi(RNA inteference)被认为是防控这种害虫的一种新策略。FTZ-F1是昆虫生长发育过程中重要的调控基因。本研究通过饲喂茄二十八星瓢虫1龄幼虫5 ng/μL dsHvFTZ-F1处理过的茄子叶片,发现幼虫中HvFTZ-F1表达水平显著降低了63.69%,抑制1龄幼虫蜕皮进入2龄,致死率高达65.00%。同时,沉默HvFTZ-F1使得20E相关基因(HvDIBHvSPOOKHvSHADOWHvSHADEHvE75)表达水平分别降低了85.36%、66.32%、52.74%、31.12%和20.76%;色素沉积相关基因(HvDDCHvTHHvebony)表达水平分别降低了88.74%、91.97%、86.15%;20E滴度和多巴胺滴度分别降低了15.97%和21.38%。综上,HvFTZ-F1在茄二十八星瓢虫的存活和生长发育中起重要作用。HvFTZ-F1可作为一种高效靶标基因用于开发基于RNAi技术的RNA生物农药,用于茄二十八星瓢虫的生物防治。

关键词: 茄二十八星瓢虫, HvFTZ-F1基因, RNAi, 20E滴度, 多巴胺滴度

Abstract: Henosepilachna vigintioctopunctata is a major pest of Solanaceae plants,causing serious economic losses to crop production in China.RNAi (RNA inteference) has been found as a novel management strategy for controlling this pest.FTZ-F1 is a key regulator of insect growth and development.In this study,by feeding 5 ng/μL dsHvFTZ-F1 solution treated eggplant leaves to 1st instar larvae of H.vigintioctopunctata,the expression level of HvFTZ-F1 was significantly reduced by 63.69%,the larvae were unable to molt normally into the 2nd instar,with the mortality as high as 65.00%.Meanwhile,silencing of HvFTZ-F1 caused the expression levels of 20E related genes,HvDIB,HvSPOOK,HvSHADOW,HvSHADE,and HvE75 was significantly reduced by 85.36%,66.32%,52.74%,31.12%,and 20.76%,respectively;the expression levels of pigmentation-related genes,HvDDC,HvTH,and Hvebony was significantly reduced by 88.74%,91.97%,and 86.15%,respectively;20E titer and dopamine titer decreased by 15.97% and 21.38%,respectively.In summary,HvFTZ-F1 plays an important role in the survival and development of H.vigintioctopunctata.HvFTZ-F1 can be used as an efficient target gene for the development of RNA biopesticide based on RNAi technology for controlling H.vigintioctopunctata.

Key words: Henosepilachna vigintioctopunctata, HvFTZ-F1, RNAi, 20E titer, dopamine titer

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