RNAi沉默HvFTZ-F1对茄二十八星瓢虫幼虫存活及发育的影响

doi:10.16409/j.cnki.2095-039x.2022.09.003

摘要/Abstract

摘要： 茄二十八星瓢虫Henosepilachna vigintioctopunctata是茄科植物上的一种重要害虫，对我国的作物生产造成了严重的经济损失。RNAi（RNA inteference）被认为是防控这种害虫的一种新策略。FTZ-F1是昆虫生长发育过程中重要的调控基因。本研究通过饲喂茄二十八星瓢虫1龄幼虫5 ng/μL dsHvFTZ-F1处理过的茄子叶片，发现幼虫中HvFTZ-F1表达水平显著降低了63.69%，抑制1龄幼虫蜕皮进入2龄，致死率高达65.00%。同时，沉默HvFTZ-F1使得20E相关基因（HvDIB、HvSPOOK、HvSHADOW、HvSHADE、HvE75）表达水平分别降低了85.36%、66.32%、52.74%、31.12%和20.76%；色素沉积相关基因（HvDDC、HvTH、Hvebony）表达水平分别降低了88.74%、91.97%、86.15%；20E滴度和多巴胺滴度分别降低了15.97%和21.38%。综上，HvFTZ-F1在茄二十八星瓢虫的存活和生长发育中起重要作用。HvFTZ-F1可作为一种高效靶标基因用于开发基于RNAi技术的RNA生物农药，用于茄二十八星瓢虫的生物防治。

关键词: 茄二十八星瓢虫, HvFTZ-F1基因, RNAi, 20E滴度, 多巴胺滴度

Abstract: Henosepilachna vigintioctopunctata is a major pest of Solanaceae plants,causing serious economic losses to crop production in China.RNAi (RNA inteference) has been found as a novel management strategy for controlling this pest.FTZ-F1 is a key regulator of insect growth and development.In this study,by feeding 5 ng/μL dsHvFTZ-F1 solution treated eggplant leaves to 1^st instar larvae of H.vigintioctopunctata,the expression level of HvFTZ-F1 was significantly reduced by 63.69%,the larvae were unable to molt normally into the 2^nd instar,with the mortality as high as 65.00%.Meanwhile,silencing of HvFTZ-F1 caused the expression levels of 20E related genes,HvDIB,HvSPOOK,HvSHADOW,HvSHADE,and HvE75 was significantly reduced by 85.36%,66.32%,52.74%,31.12%,and 20.76%,respectively;the expression levels of pigmentation-related genes,HvDDC,HvTH,and Hvebony was significantly reduced by 88.74%,91.97%,and 86.15%,respectively;20E titer and dopamine titer decreased by 15.97% and 21.38%,respectively.In summary,HvFTZ-F1 plays an important role in the survival and development of H.vigintioctopunctata.HvFTZ-F1 can be used as an efficient target gene for the development of RNA biopesticide based on RNAi technology for controlling H.vigintioctopunctata.

Key words: Henosepilachna vigintioctopunctata, HvFTZ-F1, RNAi, 20E titer, dopamine titer

中图分类号:

S188

王亚洁, 刘卓琦, 李华丽, 潘慧鹏, 杨春晓. RNAi沉默HvFTZ-F1对茄二十八星瓢虫幼虫存活及发育的影响[J]. 中国生物防治学报, 2022, 38(5): 1075-1082.

WANG Yajie, LIU Zhuoqi, LI Huali, PAN Huipeng, YANG Chunxiao. The Effects of Silencing of HvFTZ-F1 via RNAi on the Survival and Development of the 28-Spotted Potato Ladybird,Henosepilachna vigintioctopunctata Larvae[J]. Chinese Journal of Biological Control, 2022, 38(5): 1075-1082.

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