稗草致病菌——尖角突脐孢菌的生物学特性

稗草致病菌——尖角突脐孢菌的生物学特性

陈勇¹;倪汉文²;万方浩³;李晓晶²;张宏军²;李健强⁴;黄美莲⁵

1. 华南农业大学农学院, 广州　510642;2. 中国农业大学农学与生物技术学院;3. 中国农科院生物防治研究所;4. 农业部农药化学与应用技术重点开放实验室;5. 广东省农业干部学校

收稿日期:2004-01-05 修回日期:1900-01-01 出版日期:2004-11-08 发布日期:2004-11-08
通讯作者: 倪汉文

Biological Characteristics of Barnyardgrass Blight Pathogen Exserohilum monoceras

CHEN Yong;NI Han-wen;WAN Fang-hao;LI Xiao-jing;ZHANG Hong-jun;LI Jian-qiang;HUANGMei-lian

College of Agronomy,South China Agricultural University,Guangzhou 510642,China

Received:2004-01-05 Revised:1900-01-01 Online:2004-11-08 Published:2004-11-08
Contact: NI Han-wen

摘要/Abstract

摘要： 从稗草病株上分离到一尖角突脐孢菌。对该菌生物学特性的研究表明,其菌丝生长的适宜温度为25～32 ℃,最适温度为30 ℃,最适pH 为4～6 , 对光照无要求; 碳、氮、磷和硫等元素是该菌菌丝体生长的必需元素,钾和镁对菌丝体的生长有极大的促进作用,加入少量的铁和锌对菌丝生长有利,铜元素抑制该菌的生长; 缺碳和氮素明显影响菌落的生长。该菌生长最合适的速效碳源是葡萄糖,缓效碳源是玉米粉,最适无机氮源是硝酸铵,最适有机氮源是酵母粉; 分生孢子萌发的适合温度范围为20～30 ℃; 分生孢子快速萌发的酸碱条件为初始pH4～7 ,最佳初始pH 值为5 ; 光照不利于分生孢子快速萌发。

Abstract:

The pathogen Exserohilum monoceras has been isolated from Echinochloa crus-galli and its potential evaluated as a mycoherbicide against this weed. The objection of this study were to determine biological characteristics of this pathogen. For mycelium growth , the optimal temperature range was from
25 ℃to 32 ℃, and the best temperature was 30 ℃. The optimal initial pH of culture media was 4～6. Mycela could grow in either dark or light , and was independent on the leaf juice of E. crus-galli . The best carbon sources were sucrose and corn flour , and the best organic and inorganic nitrogen source were yeast flour and NH₄NO₃ 1 Adding P , S , K, Mg , Fe and Zn in the media could significantly improved the growth of mycela , but adding Cu in the media significantly inhibited the growth of the mycela. Conidia could germinate in either light or dark.

陈勇1 ;倪汉文2 ;万方浩3 ;李晓晶2 ;张宏军2 ;李健强4 ;黄美莲5 . 稗草致病菌——尖角突脐孢菌的生物学特性[J]. , DOI: .

CHEN Yong;NI Han-wen;WAN Fang-hao;LI Xiao-jing;ZHANG Hong-jun;LI Jian-qiang;HUANGMei-lian

. Biological Characteristics of Barnyardgrass Blight Pathogen Exserohilum monoceras[J]. , DOI: .