抑菌霉素A17 对12 种病原真菌及3 种害虫的生物活性

抑菌霉素A17 对12 种病原真菌及3 种害虫的生物活性

林壁润¹;胡珍娣^{1 ,2};徐汉虹²;谢双大¹;沈会芳¹;江学斌¹

1.广东省农科院植物保护研究所/ 广东省植物保护新技术重点实验室, 广州　510640 ;2. 华南农业大学昆虫毒理研究室, 广州　510642

收稿日期:2005-04-14 修回日期:1900-01-01 出版日期:2006-08-08 发布日期:2006-08-08

Pesticidal and Fungicidal Activities of Antimycin A17

LIN Bi-run;HU Zhen-di;XU Han-hong;XIE Shuang-da;SHEN Hui-fang;IANG Xue-bin

The Key Laboratory for New Technology of Plant Protection , Plant Protection Research Institute , Guangdong cademy of Agriculture Sciences , Guangzhou 510640 , China

Received:2005-04-14 Revised:1900-01-01 Online:2006-08-08 Published:2006-08-08

摘要/Abstract

摘要：

从稀有放线菌7310 菌株的发酵菌丝中经溶媒萃取和硅胶柱层析初步分离出来一种活
性成分,经结构鉴定为新结构化合物———抑菌霉素A17。抑菌霉素A17 杀虫活性结果表明,对家蝇具有很强的杀虫活性,其LC₅₀值仅为1.56μg/ ml ,并能在较短的时间内击倒供试虫体; 对菜粉蝶和小菜蛾幼虫也具有较强的杀虫活性,LC₅₀值分别为106.27 和172.9μg/ ml 。以常见的12 种病原真菌作为供试菌,在室内进行了抑菌霉素A17 抑菌活性的定性及定量分析,结果表明抑菌霉素A17 硅胶层析粗提物对12 种供试真菌中的甘薯软腐病病菌、柑桔酸腐病病菌、桃黑星病病菌、番茄早疫病病菌、番茄芽枝病病菌、莴苣菌核病病菌、荔枝霜霉病病菌、炭疽菌、弯孢霉、拟茎点霉和果腐葡萄孢的菌丝生长有明显的抑制作用,其中对拟茎点霉的活性最强,抑制中浓度仅为0.1μg/ ml 。

Abstract:

An active component was isolated from the culture of Streptomyces sp. by solvent extraction and column chromatography , and was identified as Antimycin A17 by structure elucidation. In vitro tests to Rhizopus nigricans , Geotrichum candidum , Fusicladium sp. , Alternaria solani , Sclerotinia sclerotiorum , Cladosporium sp. , Peronophthora lichii , Botrytis sp. , Fusarium oxysporum f . sp. cubense , Colletotrichum sp. , Phomopsis sp. , Curvularia sp. indicated that Antimycin A17 was highly toxic to eleven ungi , especially Phomopsis with IC₅₀ 0.1μg/ ml lower than those to others , and Antimycin A17 toxic to Musca domestica with LC₅₀ 1.56μg/ ml .

中图分类号:

林壁润1 ;胡珍娣1 ,2 ;徐汉虹2 ;谢双大1 ;沈会芳1 ;江学斌1 . 抑菌霉素A17 对12 种病原真菌及3 种害虫的生物活性[J]. , DOI: .

LIN Bi-run;HU Zhen-di;XU Han-hong;XIE Shuang-da;SHEN Hui-fang;IANG Xue-bin. Pesticidal and Fungicidal Activities of Antimycin A17[J]. , DOI: .