Abstract: The tested four lepidopteran cell lines (SL-ZSU-1, Tn-H5, Sf9 and Ha-AM1) can not grow in L-15+5% FBS (L-15), or L-15+350mg/L proline+5% FBS (L-15-P) at pH 6.5, but they can grow in L-15 medium supplemented with 1g/L glucose and 5%FBS (L-15-G), L-15+350mg/L proline+1g/Lglucose+5% FBS (L-15-PG), or L-15+3g/L lactalbumin hydrolysate+3g/L yeast extract+1g/L glucose+5%FBS (L-15-LYG). The time needed for adaptation to these modified L-15 media was different. The four cell lines could be rapidly adapted to L-15-LYG medium, but the time needed for adaptation to L-15-PG and L-15-G was one month and 45-60d, respectively. The growth of the four cell lines in L-15-LYG medium was equal to that in TNM-FH medium. The titers of Anagrapha falcifera multiple nucleopolyhedrovirus (AfaMNPV) for Tn-H5 and Sf9 cell lines cultured in L-15-LYG medium were as good as that in TNM-FH medium, but the production of occlusion bodies (OBs) was decreased.