Abstract: Wuyiencin was a nucleotide antibiotic produced by Streptomyces ahygroscopicus var. wuyiensis. In order to develop Wuyiencin soluble powder formulation, the absorbability of four carriers including attapulrite, kaolinite, diatomite and white carbon black were tested in this research. Results showed that the adsorbability of white carbon black to Wuyiencin fermentation filtrate was the best, reaching 3.52 ml/g as saturation absorbance. Dispersibility and wettability of Wuyiencin soluble powder prepared with nonionic surfactant GY-JM6180 was better than that with anion surfactant NNO. Control effect of 2% Wuyiencin soluble powder formulation with adjuvant GY-JM6180 against wheat powdery mildew was 73.7% in contrast to 31.5% by Wuyiencin fermentation filtrate when sprayed at the same dosage.