60Co-NTG复合诱变选育丁烯基多杀菌素高产菌株及其杀虫活性

doi:10.16409/j.cnki.2095-039x.2023.01.027

中国生物防治学报 ›› 2024, Vol. 40 ›› Issue (2): 299-309.DOI: 10.16409/j.cnki.2095-039x.2023.01.027

• 研究论文 • 上一篇

⁶⁰Co-NTG复合诱变选育丁烯基多杀菌素高产菌株及其杀虫活性

徐周钦^1,2, 郭超², 李金萍³, 张逍遥², 王珂慧², 何志平¹, 刘兴泉¹, 王超²

1. 浙江农林大学食品与健康学院, 杭州 311300;
2. 国家粮食和物资储备局科学研究院粮油加工研究所, 北京 100037;
3. 北京市植物保护站, 北京 100029

收稿日期:2023-02-10 发布日期:2024-04-28
通讯作者: 刘兴泉,博士,教授,E-mail:liuxq@zafu.edu.cn;王超,博士,副研究员,E-mail:wc@ags.ac.cn。
作者简介:徐周钦,硕士研究生,E-mail:1347199738@qq.com。
基金资助:
国家重点研发计划（ 2023YFA0914700）；中央公益性科研院所专项课题（ JY2311）；企业合作技术开发项目（ H20054-1）

Breeding of Butenyl-spinosyns High Yielding Strain by ⁶⁰Co-NTG Compound Mutation and Its Insecticidal Activity

XU Zhouqin^1,2, GUO Chao², LI Jinping³, ZHANG Xiaoyao², WANG Kehui², HE Zhiping¹, LIU Xingquan¹, WANG Chao²

1. College of Food and Health, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China;
2. Institute of Cereal&Oil Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, China;
3. Beijing Plant Protection Station, Beijing 100029, China

Received:2023-02-10 Published:2024-04-28

摘要/Abstract

摘要： 为获得稳定高产的丁烯基多杀菌素生产菌株并明确其对农业害虫的杀灭效果，以前期研究获得的须糖多孢菌Saccharopolyspora pogona ASAGF19为初始菌株，通过⁶⁰Co-γ射线和亚硝基胍复合诱变得到1株遗传性状稳定的高产突变株ASAGF30A11，较初始菌株产量提高了61.26%，3次传代产量变化幅度小于10.00%。对高产菌株发酵液进行分离纯化获得了丁烯基多杀菌素粗品，配制成1.5 g/L浓度的母液进行稀释，对蓟马、蚜虫以及小菜蛾进行室内毒力测定，得出其对3种供试害虫的LC₅₀分别为1.92 mg/L、1.23 mg/L和0.27 mg/L。经过比较，丁烯基多杀菌素对蓟马的杀灭效果优于多杀菌素（ LC₅₀为0.57 mg/L）和乙基多杀菌素（ LC₅₀为0.64 mg/L）；对蚜虫的杀灭效果优于多杀菌素（ LC₅₀为72.60 mg/L），低于阿维菌素（ LC₅₀为0.14 mg/L）；对小菜蛾的杀灭效果优于多杀菌素（ LC₅₀为2.87 mg/L），低于乙基多杀菌素（ LC₅₀为0.05 mg/L）。本研究首次探索了⁶⁰Co-γ射线和亚硝基胍复合诱变对须糖多孢菌的影响，并获得了丁烯基多杀菌素对3种农业害虫的防效，为新型生物杀虫剂对农业害虫的防治提供了一些理论依据。

关键词: 须糖多孢菌, ⁶⁰Co-γ射线, 亚硝基胍, 复合诱变, 丁烯基多杀菌素

Abstract: In order to obtain stable and high-yield butenyl-spinosyn-producing strains and determine its lethal efficiency against agricultural pests,a high-yield mutant strain ASAGF30A11 with stable genetic traits was obtained by compound mutagenesis of a parent strain Saccharopolyspora pogona ASAGF19 screened from the previous study using ⁶⁰Co-γ-ray and nitrosoguanidine.The new strain increased yield by 61.26% compared with the parent strain,and the yield variation of 3 generations was less than 10%.The crude butenyl-spinosyn was purified from the fermentation broth of the high-yield strain,and then diluted into the mother liquor with a concentration of 1.5 g/L.The insecticidal activity against Thrips palmi Karny,aphids and Plutella xylostella was investigated and the LC₅₀ was determined to be 1.92 mg/L,1.23 mg/L and 0.27 mg/L,respectively.By comparison,butenyl-spinosyn was of a higher lethal efficiency against T.palmi than spinosyn (LC₅₀ 0.58 mg/L) and spinetoram (LC₅₀ 0.64 mg/L),a higher lethal efficiency against aphids than spinosyn (LC₅₀ 72.60 mg/L) but equal efficiency with avermectin (LC₅₀ 0.14 mg/L),and a higher lethal efficiency against P.xylostella than spinosyn (LC₅₀ 2.9 mg/L) but lower efficiency than spinetoram (LC₅₀ 0.05 mg/L).This study explored the effect of compound mutagenesis using ⁶⁰Co-γ-ray and nitrosoguanidine on S.pogona for the first time and measured the laboratory lethal efficiency of butenyl-spinosyn against three agricultural pests,which serves as part of the theoretical basis for the control of agricultural pests with new biological insecticides.

Key words: Saccharopolyspora pogona, ⁶⁰Co-γ-ray, NTG, combined mutagenesis, butenyl-spinosyns

中图分类号:

S482.3

徐周钦, 郭超, 李金萍, 张逍遥, 王珂慧, 何志平, 刘兴泉, 王超. ⁶⁰Co-NTG复合诱变选育丁烯基多杀菌素高产菌株及其杀虫活性[J]. 中国生物防治学报, 2024, 40(2): 299-309.

XU Zhouqin, GUO Chao, LI Jinping, ZHANG Xiaoyao, WANG Kehui, HE Zhiping, LIU Xingquan, WANG Chao. Breeding of Butenyl-spinosyns High Yielding Strain by ⁶⁰Co-NTG Compound Mutation and Its Insecticidal Activity[J]. Chinese Journal of Biological Control, 2024, 40(2): 299-309.

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⁶⁰Co-NTG复合诱变选育丁烯基多杀菌素高产菌株及其杀虫活性

Breeding of Butenyl-spinosyns High Yielding Strain by ⁶⁰Co-NTG Compound Mutation and Its Insecticidal Activity

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