诱导子对黄曲霉生物合成乌头碱的诱导作用研究

doi:10.16409/j.cnki.2095-039x.2024.02.015

中国生物防治学报 ›› 2024, Vol. 40 ›› Issue (2): 469-476.DOI: 10.16409/j.cnki.2095-039x.2024.02.015

• 研究论文 • 上一篇

诱导子对黄曲霉生物合成乌头碱的诱导作用研究

王凯, 袁希元, 张明月, 田硕, 何璐

沈阳农业大学植物保护学院, 沈阳 110866

收稿日期:2023-05-29 发布日期:2024-04-28
通讯作者: 何璐,博士,教授,E-mail:87478@163.com。
作者简介:王凯,博士,讲师,E-mail:wangkai12@syau.edu.cn;袁希元,硕士研究生; E-mail:yuanxiyuan123@163.com。
基金资助:
辽宁省民生科技计划（ 2021JH2/10200016）

Induction of Aconitine Biosynthesis in Aspergillus flavus by Inducers

WANG Kai, YUAN Xiyuan, ZHANG Mingyue, TIAN Shuo, HE Lu

College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China

Received:2023-05-29 Published:2024-04-28

摘要/Abstract

摘要： 为探索不同诱导子对生物合成乌头碱产量的影响，本研究在黄曲霉Aspergillus flavus的液体发酵过程中分别加入金属诱导子（ Co²⁺、Ag⁺和Cu²⁺）和化学诱导子（茉莉酸甲酯、水杨酸和硝普钠），以及酵母提取物、种子提取物和共生细菌发酵液等生物诱导子进行诱导试验。试验结果表明，Ag⁺、Cu²⁺和Co²⁺都对乌头碱的生物合成有促进作用，其中Ag⁺浓度为50 μmol/L时，乌头碱产量与对照组相比提高了10倍。化学诱导子MJ和SA诱导效果显著，其中100 μmol/L MJ的加入能显著提高乌头碱的产量，产量为20.74 mg/L，为对照组的11倍。而酵母提取物、种子提取物和共生细菌发酵液对乌头碱的产量诱导效果均不显著。本论文探究了不同诱导子对黄曲霉生物合成乌头碱的诱导作用，进而明确不同诱导子及不同诱导浓度对乌头碱生物合成产量的影响。

关键词: 诱导子, 黄曲霉菌, 乌头碱, 生物合成

Abstract: In order to explore the effect of different inducers on the yield of biosynthesis aconitine,the present study was carried out in the liquid fermentation of Aspergillus flavus by adding metal inducers (Co²⁺,Ag⁺ and Cu²⁺) and chemical inducers (Methyl Jasmonate,MJ,Salicylic Acid,SA and Sodium nitroprusside,SND),as well as YE (Yeast extract),SE (Seed extract) and Bac (Symbiotic bacterial fermentation broth).The results showed that Ag⁺,Cu²⁺ and Co²⁺ all could promote the biosynthesis of aconitine,in which Ag⁺ at 50 μmol/L increased the aconitine yield by 10-fold compared with the control.Induction effects of the chemical inducers MJ and SA were significant,in which the addition of 100 μmol/L MJ significantly increased the production of aconitine with a yield of 20.74 mg/L,which was 11-fold higher than that of the control,while YE,SE and Bac did not have any significant effect on the biosynthesis of aconitine by Aspergillus flavus.

Key words: inducer, Aspergillus flavus, aconitine, biosynthesis

中图分类号:

S476

王凯, 袁希元, 张明月, 田硕, 何璐. 诱导子对黄曲霉生物合成乌头碱的诱导作用研究[J]. 中国生物防治学报, 2024, 40(2): 469-476.

WANG Kai, YUAN Xiyuan, ZHANG Mingyue, TIAN Shuo, HE Lu. Induction of Aconitine Biosynthesis in Aspergillus flavus by Inducers[J]. Chinese Journal of Biological Control, 2024, 40(2): 469-476.

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诱导子对黄曲霉生物合成乌头碱的诱导作用研究

Induction of Aconitine Biosynthesis in Aspergillus flavus by Inducers

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