球孢白僵菌病毒BbCV2的流行及其遗传多样性

doi:10.16409/j.cnki.2095-039x.2023.01.048

中国生物防治学报 ›› 2024, Vol. 40 ›› Issue (3): 592-599.DOI: 10.16409/j.cnki.2095-039x.2023.01.048

• 研究论文 • 上一篇

球孢白僵菌病毒BbCV2的流行及其遗传多样性

刘洪宇^1,2, 牛昕泽^1,2, 孙旸¹, 赵宇², 路杨², 隋丽², 李启云^1,2, 张正坤²

1. 吉林农业大学生命科学学院, 长春 130118;
2. 吉林省农业科学院植物保护研究所/农业农村部东北作物有害生物综合治理重点实验室/吉林省农业微生物重点实验室, 长春 136100

收稿日期:2023-03-14 发布日期:2024-06-07
通讯作者: 张正坤,研究员,E-mail:zhangzhegnkun1980@126.com;李启云,研究员,E-mail:qyli1225@126.com。
作者简介:刘洪宇,硕士研究生,E-mail:3090433121@qq.com;牛昕泽,硕士研究生,E-mail:xinzeniu@163.com

Epidemic and Genetic Diversity of Beauveria bassiana chrysovirus 2

LIU Hongyu^1,2, NIU Xinze^1,2, SUN Yang¹, ZHAO Yu², LU Yang², SUI Li², LI Qiyun^1,2, ZHANG Zhengkun²

1. College of Life Sciences, Jilin Agricultural University, Changchun 130118, China;
2. Institute of Plant Protection, Jilin Academy of Agricultural Science/Jilin Key Laboratory of Agricultural Microbiology/Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Gongzhuling 136100, China

Received:2023-03-14 Published:2024-06-07
Contact: 吉林省科技厅中青年科技创新创业卓越人才（团队）项目（创新类)(20230508011RC）
Supported by:
10.16409/j.cnki.2095-039x.2023.01.048

摘要/Abstract

摘要： 球孢白僵菌Beauveria bassian是一种重要的虫生真菌，广泛用于害虫生物防治，毒力退化一直是制约其应用的重要因素。真菌病毒能够影响寄主真菌毒力。为明确一株能够降低寄主毒力的产黄青霉科病毒Beauveria bassiana chrysovirus 2（BbCV2）真菌病毒在田间球孢白僵菌种群中流行性规律，本试验收集了来源于吉林省7个不同地点采集的寄主为亚洲玉米螟Ostrinia furnacalis的球孢白僵菌菌株，通过dsRNA提取和PCR验证BbCV2的感染情况，并进行遗传多样性分析。结果表明，不同采集地的球孢白僵菌均检测到dsRNA病毒感染，尤其是BbCV2在吉林省内分布广泛，菌株带毒率为70%～100%。对BbCV2依赖RNA的RNA聚合酶基因（RdRp）进行遗传多样性分析发现，该病毒不同分离物间RdRp的核苷酸序列和氨基酸序列高度保守，相似度高达99.78%和99.98%，该病毒遗传相对稳定，并且其分布和遗传进化与地理位置无关。由此可见，BbCV2的流行是自然发生的，可能是造成球孢白僵菌田间毒力退化的重要因素，并且球孢白僵菌对该病毒适应性较强，对病毒的遗传选择压力不明显。

关键词: 球孢白僵菌, 真菌病毒, 遗传进化, 生物防治

Abstract: Beauveria bassiana, an important entomogenous fungus, is widely used in biological control of pests. However, the pathogenicity degradation is an important factor restricting its application. Mycovirus could affect the pathogenicity of host fungi. A mycovirus Beauveria bassiana chrysovirus2 (BbCV2) reduces the virulence of host fungi B. bassiana population in the field. With the aim to understand the epidemic of the mycovirus, the infection of mycovirus BbCV2 in strains of B. bassiana isolated from Ostrinia furnacalis collected from 7 different sites in Jilin Province was verified by dsRNA extraction and RT-PCR methods, and the genetic diversity of BbCV2 was analyzed. The results showed that the infection of dsRNA mycovirus, especially the BbCV2, were universal in all samples, with the BbCV2 infection rate ranging from 70% to 100%. The genetic diversity of the RNA-dependent RNA polymerase (RdRp) gene of BbCV2 showed a highly conserved similarity, of which the nucleic acid sequence and amino acid sequence were up to 99.78% and 99.98%, respectively, indicating the virus is genetically stable. Further, the virus distribution and genetic evolution showed no relationship with geographical location. Therefore, the mycovirus BbCV2 is characterized by natural prevalence, which may have caused the pathogenicity degradation in the field. And B. bassiana adapts well to the mycovirus and exerts no strong selective pressure on the virus genetic evolution.

Key words: Beauveria bassiana, mycovirus, genetic evolution, biocontrol

中图分类号:

S476.2

刘洪宇, 牛昕泽, 孙旸, 赵宇, 路杨, 隋丽, 李启云, 张正坤. 球孢白僵菌病毒BbCV2的流行及其遗传多样性[J]. 中国生物防治学报, 2024, 40(3): 592-599.

LIU Hongyu, NIU Xinze, SUN Yang, ZHAO Yu, LU Yang, SUI Li, LI Qiyun, ZHANG Zhengkun. Epidemic and Genetic Diversity of Beauveria bassiana chrysovirus 2[J]. Chinese Journal of Biological Control, 2024, 40(3): 592-599.

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球孢白僵菌病毒BbCV2的流行及其遗传多样性

Epidemic and Genetic Diversity of Beauveria bassiana chrysovirus 2

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