新洋葱伯克霍尔德氏菌YM12的分离鉴定及其对黄瓜软腐病的防治效果

doi:10.16409/j.cnki.2095-039x.2024.02.059

摘要/Abstract

摘要： 黄瓜软腐病是由巴西果胶杆菌Pectobacterium brasiliense引起的一种细菌性病害，该病害发生严重且难防难控，以筛选具有高效抑菌作用的生防微生物为目的，本研究在黄瓜根际土壤中分离得到了一株对巴西果胶杆菌具有显著抑制效果的菌株YM12。经形态学观察、生理生化特征测定及多基因系统发育树分析，鉴定其为新洋葱伯克霍尔德氏菌Burkholderia cenocepacia；抗生素合成基因检测结果表明，菌株YM12具有产生硝吡咯菌素pyrrolnitrin与藤黄绿脓菌素pyoluteorin的能力；其他生防性状分析表明，YM12具有溶磷能力，还可产生蛋白酶及嗜铁素。经抑菌谱分析，菌株YM12能够抑制5种病原细菌及3种病原真菌的生长。盆栽试验结果表明，菌株YM12对黄瓜软腐病具有明显的防治效果，防效可达62.84%，优于对照药剂春雷霉素。综上，菌株YM12是目前首次报道的对细菌性软腐病具有良好生防效果的新洋葱伯克霍尔德氏菌。

关键词: 新洋葱伯克霍尔德氏菌, 黄瓜软腐病, 抑菌活性, 生物防治

Abstract: Soft rot of cucumber is a bacterial disease caused by Pectobacterium brasiliense. The disease is serious and difficult to control. In order to screen biocontrol microorganisms with high antibacterial effect, a strain YM12 with significant inhibitory effect on P. brasiliense was isolated from cucumber rhizosphere soil. Through morphological observation, physiological and biochemical determination and multi-gene phylogenetic tree analysis, the strain was identified as Burkholderia cenocepacia. Analysis of antibiotic synthesis-related genes showed that strain YM12 might also produce Pyrrolnitrin and Pyhluteorin. Analysis of biocontrol traits characteristics confirmed that YM12 had the ability to produce phosphatase protease and pyoverdine. The strain had antagonistic effects on 5 pathogenic bacteria and 3 pathogenic fungi by antimicrobial spectrum analysis. Pot experiment showed that strain YM12 had obvious control effect on cucumber soft rot, with the efficacy of 62.84%.In summary, strain YM12 is the first reported B. cenocepacia with biocontrol effect on bacterial soft rot, which has high biocontrol research value and development and application potential.

Key words: Burkholderia cenocepacia, cucumber bacterial soft rot, antibacterial activity, biological control

中图分类号:

S476

卫鑫辰, 王巧, 石彬, 谢学文, 石延霞, 柴阿丽, 李宝聚, 李磊. 新洋葱伯克霍尔德氏菌YM12的分离鉴定及其对黄瓜软腐病的防治效果[J]. 中国生物防治学报, 2024, 40(5): 1088-1098.

WEI Xinchen, WANG Qiao, SHI Bin, XIE Xuewen, SHI Yanxia, CHAI Ali, LI Baoju, LI Lei. Isolation and Identification of Burkholderia cenocepacia YM12 and Its Control Effect on Cucumber Soft Rot Disease[J]. Chinese Journal of Biological Control, 2024, 40(5): 1088-1098.

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