广西首次发现外来入侵害虫桉树叶疱球角姬小蜂（膜翅目：姬小蜂科）

doi:10.16409/j.cnki.2095-039x.2025.07.002

中国生物防治学报 ›› 2025, Vol. 41 ›› Issue (6): 1522-1529.DOI: 10.16409/j.cnki.2095-039x.2025.07.002

• 天敌昆虫物种鉴定 • 上一篇

广西首次发现外来入侵害虫桉树叶疱球角姬小蜂（膜翅目：姬小蜂科）

李俊珏¹, 谢金婷¹, 周乐明¹, 徐秀¹, 杨振德¹, 杨忠岐²

1. 广西大学林学院, 南宁 530001;
2. 中国林业科学研究院森林生态环境与自然保护研究所/国家林草局森林保护学重点实验室, 北京 100091

收稿日期:2024-12-09 发布日期:2025-12-22
通讯作者: 杨振德, 杨忠岐
作者简介:李俊珏，硕士研究生，E-mail：13978755183@163.com。通信作者，杨振德，博士，教授，E-mail:dzyang68@126.com；杨忠岐，研究员，E-mail：yangzhqi@126.com。
基金资助:
国家自然科学基金（32360390，31971664）

First Discovery of Invasive Pest Ophelimus bipolaris (Hymenoptera: Eulophidae) in Guangxi

LI Junjue¹, XIE Jinting¹, ZHOU Leming¹, XU Xiu¹, YANG Zhende¹, YANG Zhongqi²

1. College of Forestry, Guangxi University, Nanning 530001, China;
2. Key Laboratory of Forest Protection of National Forestry and Grassland Administration/Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China

Received:2024-12-09 Published:2025-12-22

摘要/Abstract

摘要： 本文对广西发现可在桉树叶片上形成泡囊状虫瘿，导致叶片变形、枯萎、脱落的一种害虫进行形态学和分子鉴定，确定害虫种类，为野外识别、监测与防治提供参考。在广西贺州市昭平县，采集DH299-5品系桉树上带有虫瘿的叶片30片，置于保鲜袋中密封，带回实验室每天检查桉树叶疱球角姬小蜂羽化状况，进行收集、统计与鉴定。通过体视镜与超景深三维显微系统对小蜂进行形态学鉴定，使用DNA提取试剂盒提取小蜂DNA，对COI和28S rRNA两个片段进行测序，上传NCBI数据库比对后下载同属物种，采用最大似然法，以桉树扁角金绿姬小蜂Closterocerus chamaeleon作为外群，构建系统发育树。从5片叶子中共收集到小蜂984头，其COI和28S rRNA的序列长度分别为640 bp和435 bp，经Blastn同源性比对，与Ophelimus bipolaris相似度最高，结合形态学鉴定，确定该害虫为桉树叶疱球角姬小蜂O. bipolaris。桉树叶疱球角姬小蜂具有侵害多种桉树叶片的能力，导致叶片形成虫瘿并大量脱落，需加强检疫与监测，采取防治措施，特别是利用自然天敌进行生物防治，减少化学农药使用，保障桉树产业健康发展。

关键词: 桉树, 桉树叶疱球角姬小蜂, 外来入侵害虫, 形态学特征, DNA 鉴定

Abstract: An insect species that can induce vesicular gall formation on Eucalyptus leaves, resulting in leaf deformation, desiccation, and abscission, was identified using morphological and molecular methods, which may provide reference for field identification, monitoring, and management. Thirty Eucalyptus leaves with galls from the DH299-5 strain were collected in Zhaoping County, Hezhou City, Guangxi Province, and sealed in airtight preservation bags. Daily inspections were conducted to monitor the insect emergence and to collect and enumerate the insects for identification. Morphological identification was conducted using a stereomicroscope and an ultra-depth-of-field three-dimensional microscope system. DNA extraction was performed using a DNA extraction kit, followed by sequencing of the cytochrome oxidase subunit I (COI) and 28S ribosomal RNA (28S rRNA) genes. The sequences were uploaded to the National Center for Biotechnology Information (NCBI) database for BLAST comparison, and congeneric species sequences were downloaded. A phylogenetic tree was constructed using the maximum likelihood method, with Closterocerus chamaeleon as the outgroup. A total of 984 insects were collected from five leaves. The sequence lengths of COI and 28S rRNA were 640 bp and 435 bp, respectively. Blastn homology comparison revealed the highest similarity to Ophelimus bipolaris. Combined with morphological identification, the pest was confirmed as O. bipolaris. O. bipolaris has a broad host range among various Eucalyptus species, causing gall formation and extensive leaf shedding. Strengthened quarantine measures, intensified monitoring, and preventive actions, particularly biological control utilizing natural enemies and reducing chemical pesticide use, are crucial for safeguarding the healthy development of the Eucalyptus industry.

Key words: Eucalyptus spp., Ophelimus bipolaris, invasive pest, morphological identification, DNA examination

中图分类号:

S476.3

李俊珏, 谢金婷, 周乐明, 徐秀, 杨振德, 杨忠岐. 广西首次发现外来入侵害虫桉树叶疱球角姬小蜂（膜翅目：姬小蜂科）[J]. 中国生物防治学报, 2025, 41(6): 1522-1529.

LI Junjue, XIE Jinting, ZHOU Leming, XU Xiu, YANG Zhende, YANG Zhongqi. First Discovery of Invasive Pest Ophelimus bipolaris (Hymenoptera: Eulophidae) in Guangxi[J]. Chinese Journal of Biological Control, 2025, 41(6): 1522-1529.

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广西首次发现外来入侵害虫桉树叶疱球角姬小蜂（膜翅目：姬小蜂科）

First Discovery of Invasive Pest Ophelimus bipolaris (Hymenoptera: Eulophidae) in Guangxi

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