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胶孢炭疽菌株QZ-97a分生孢子货架期及对温度的耐受力

朱 秦1;强 胜2   

  1. 南京农业大学杂草研究室, 南京210095
  • 收稿日期:2003-06-09 修回日期:1900-01-01 出版日期:2004-05-08 发布日期:2004-05-08
  • 通讯作者: 朱 秦

The Shelf-life and High Temperature Tolerance of Conidia of Colletotrichum gloeosporioides f.sp. veronicae

ZHU Qin;QIANG Sheng   

  1. Weed Research Laboratory, Nanjing Agricultural University, Jiangsu 210095, China
  • Received:2003-06-09 Revised:1900-01-01 Online:2004-05-08 Published:2004-05-08
  • Contact: ZHU Qin

摘要: 将不同含水量的胶孢炭疽菌婆婆纳专化型菌株QZ-97a分生孢子粉置于4℃和室温下,密闭或开放贮存。结果表明,4℃下密闭贮藏6个月,分生孢子萌发率和芽管长度无明显降低,室温贮藏降低也不明显。贮藏10个月后,各种贮藏方式孢子的活力都显著下降。但以4℃、含水量4%、密闭贮藏孢子活力较为稳定,420d孢子萌发率为70.4%,芽管长度为贮藏60d时的43.2%。含水量9.6%、开放室温下贮藏的孢子粉活力下降最大。因此,温度和孢子粉含水量是影响孢子货架期的两个主要因素。分生孢子粉对温度有一定的耐受性,随温度增高,敏感性增强,60℃下分生孢子的活力下降显著,处理6min,孢子芽管长度较对照下降50%。

Abstract: While the fresh conidial powder of Collectotrichum gloeosporioides f. sp. veronicae was stored at 4℃ and room temperature in open or close capsule, germination rates and length of germ tube of conidia were examined at 15 and 60d intervals, respectively. Results showed that there were no significant differences in germination rates and length of germ tube of conidia stored at 4℃ and room temperature within 6 months. However, the germination rates and length of germ tube of conidia decreased significantly after storage for over 10 months at 4℃ or room temperature. In general, the conidia viability were most stable when the conidia powder with 4.0% water content was stored closely at 4℃ because the germination rates were 70.4% and the length of germ tube decreased only by 43.2% by the end of 420d comparing to that by 60d. Whereas, the conidia powder with 9.6% water content stored openly at room temperature were declined the most obviously, the corresponding values were 35.5% and 12.7% respectively. Therefore, storage temperature and water content of conidia were two key factors, influencing the shelf-life of C. gloeosporioides f.sp. veronicae conidia. The conidia were heat-treated in oven at 40-60℃ for 15-120 min to observe tolerance to high temperature of conidia. Results showed that the conidia powder was partly tolerant to temperature, while the susceptivity increased obviously with temperature rising. A regression analysis showed that the length of germ tube of conidia were decreased by 50% when heat-treated at 60℃ for 6 min comparing with that of control.

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