Abstract: This paper deals with liquid culture of entomopathogenic nemotode Steinernema carpocapsae A24 in air-lift internal loop bioreactor, bubble tank bioreactor and stirred tank bioreactor on the base of flask culture. The result indicates that the highest yield of infective nematode can be obtained in air lift internal loop bioreactor. The research on the effect of amount of aeration on the nematode culture results in the optium amount of aeration: 1.0vvm during 1~6 d, 0.8vvm during 7~12 d and 1 5vvm during 13~18 d.