Abstract: Non-ribosomal peptides are small molecular compounds mainly composed of specific or modified amino acids and oxyacids. They are synthesized via thiotemplate multienzyme mechanism of multifunctional enzyme complex system other than on ribosome. NRPs show a broad range of biological activities, functions of non-ribosomal peptides mainly include antibiotics, immunity inhibitor, cell proliferation inhibitor, siderophore or ionophore, etc. There are eight primary kinds of non-ribosomal peptides produced by entomogenous fungi, inculding efrapeptin, cyclosporin, destruxin, beauvericin, enniatin, bassianolide, beauverolide and serinocyclin. The types, structures, activity, mechanism of these NRPs were reviewed to provide a reference for researchers interested in NPRs.