Abstract: We assessed the control effects of three fungicide-fertilizer dual purpose bio-organic manure, the mixture of bio-organic fertilizer with compound Trichoderma fungicide,single strain Trichoderma fungicide and Bacillus subtilis fungicide, on Fusarium wilt of banana. The results showed that three fertilizers had inhibitory effects on Fusarium wilt disease with the disease index of Fusarium wilt reduced by 31.8%-50.0%, the controlling efficiency reached to 31.8%—49.6%, and the activity of β-1,3-glucanase in banana root-tip significantly increased 18.95%—37.13% comparing to the control. The population of Fusarim oxysporum, microorganism community in the rhizospheric soil, and community structure of soil microorganism were changed upon application the three bio-organic fertilizers. Comparing to the control, population of rhizospheric bacteria and actinomycetes increased 2.75—4.30 and 2.14—2.33 times, respectively; population of fungi and Fusarim oxysporum in the rhizospheric soil decreased 19.07%—23.26% and 2.18%—80.27%, respectively. The contents of soil microbial biomass carbon and nitrogen increased 760-780 mg·kg^-1 and 13.2-15.8 mg·kg^-1, respectively.