枯草芽胞杆菌PTS-394可湿性粉剂对辣椒根系及根际微生态影响

doi:10.16409/j.cnki.2095-039x.2018.02.017

中国生物防治学报 ›› 2018, Vol. 34 ›› Issue (2): 287-293.DOI: 10.16409/j.cnki.2095-039x.2018.02.017

枯草芽胞杆菌PTS-394可湿性粉剂对辣椒根系及根际微生态影响

乔俊卿¹, 罗德旭², 彭彪^1,3, 孙玉东², 刘永锋¹, 刘邮洲¹

1. 江苏省农业科学院植物保护研究所, 南京 210014;
2. 江苏省淮安市农业科学研究院, 淮安 223001;
3. 扬州大学生物科学与技术学院, 扬州 225009

收稿日期:2017-11-01 出版日期:2018-04-08 发布日期:2018-04-13
通讯作者: 刘邮洲,博士,研究员,E-mail:shitouren88888@163.com。
作者简介:乔俊卿,博士,副研究员,E-mail:junqingqiao@hotmail.com;罗德旭,硕士,副研究员,E-mail:luodexu2002@163.com
基金资助:
江苏省农业自主创新基金（CX-16-1021）；国家重点研发计划（2016YFD0201007）

The Evaluation of Impact on Pepper Root and Rhizosphere Microbial Community Exerted by Bacillus subtilis PTS-394 Water Powder

QIAO Junqing¹, LUO Dexu², PENG Biao^1,3, SUN Yudong², LIU Yongfeng¹, LIU Youzhou¹

1. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. Huai'an Academy of Agricultural Sciences, Huai'an 223001, China;
3. College of Biology Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2017-11-01 Online:2018-04-08 Published:2018-04-13

摘要/Abstract

摘要： 本研究通过测定辣椒根系活力及生长情况，检测根际土壤可培养微生物数量和土壤酶活性等，以期明确枯草芽胞杆菌PTS-394可湿性粉剂对辣椒根系及根际微生态的影响。结果显示，枯草芽胞杆菌PTS-394可湿性粉剂（1:100）稀释菌液能够显著促进辣椒根系的伸长，促生比例达8.61%，对辣椒根重量也有促进作用，增加比例为19.2%，同时还可以显著提高辣椒的根系活力，尤其是在施用后的第12和24 h；而菌株PTS-394（1:1000）稀释菌液虽然对辣椒的根长、重量和根系活力也都有促进作用，但未达到显著效果。此外，稀释100倍的枯草芽胞杆菌PTS-394可湿性粉剂对辣椒根际土壤中的真菌和放线菌含量均具有显著抑制作用，最高抑制率分别达63.6%和69.5%，而对细菌则无显著影响。同时，该稀释浓度的PTS-394菌液还具有促进土壤脲酶和蔗糖酶活性的作用。本研究结果不仅扩展了枯草芽胞杆菌PTS-394可湿性粉剂的施用范围，丰富了促生机理，更为其在设施辣椒栽培中的安全使用提供了理论基础。

关键词: 枯草芽胞杆菌可湿性粉剂, 辣椒, 根系活力, 根际微生物群落, 土壤酶活

Abstract: In order to clarify the effect on plant root of Bacillus subtilis PTS-394 water powder and its impact on the microbial community in rhizosphere, the pepper root growth, root activity, the amount of microbial of rhizosphere and soil enzyme activity were measured. The results showed that B. subtilis PTS-394 WP (1:100) dilution has ability to promote the growth of pepper root and can increase the root dry weight and root activity. The root length and weight were about 11.60 cm and 0.155 g at 25 day after application of PTS-394 WP (1:100) and higher 8.61% and 19.2% than control respectively. The root activity was stimulated significantly at 12 and 24 h after treatment with PTS-394 WP (1:100). Then, the root activity was decreased to the almost same level with control at sampling time point 96 h. The lower PTS-394 WP (1:1000) dilution has a same effect on the pepper root, but not significantly. After the application of PTS-394 WP (1:100), the amount of rhizosphere soil fungus and actinomycetes were suppressed, the highest inhibitory rates were 63.6% and 69.5% respectively; while the bacterium amount was almost the same as control. In another hand, PTS-394 WP (1:100) is able to increase the soil urease and sucrase activity. All above results were not only expand the use of PTS-394 WP, but enriched the growth promotion mechanisms of B. subtilis PTS-394 on pepper and provided a theoretical basis for the safety of application in the field.

Key words: Bacillus subtilis water powder, pepper, root activity, microbial community in rhizosphere, soil enzyme activity

中图分类号:

S476

乔俊卿, 罗德旭, 彭彪, 孙玉东, 刘永锋, 刘邮洲. 枯草芽胞杆菌PTS-394可湿性粉剂对辣椒根系及根际微生态影响[J]. 中国生物防治学报, 2018, 34(2): 287-293.

QIAO Junqing, LUO Dexu, PENG Biao, SUN Yudong, LIU Yongfeng, LIU Youzhou. The Evaluation of Impact on Pepper Root and Rhizosphere Microbial Community Exerted by Bacillus subtilis PTS-394 Water Powder[J]. journal1, 2018, 34(2): 287-293.

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枯草芽胞杆菌PTS-394可湿性粉剂对辣椒根系及根际微生态影响

The Evaluation of Impact on Pepper Root and Rhizosphere Microbial Community Exerted by Bacillus subtilis PTS-394 Water Powder

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