β-氨基丁酸和茄青枯菌hrp-突变体协同防治辣椒疫病研究

doi:10.16409/j.cnki.2095-039x.2017.04.012

中国生物防治学报 ›› 2017, Vol. 33 ›› Issue (4): 519-524.DOI: 10.16409/j.cnki.2095-039x.2017.04.012

β-氨基丁酸和茄青枯菌hrp-突变体协同防治辣椒疫病研究

杨宇红, 茆振川, 凌键, 谢丙炎

中国农业科学院蔬菜花卉研究所, 北京 100081

收稿日期:2017-04-07 出版日期:2017-08-08 发布日期:2017-08-11
通讯作者: 杨宇红
作者简介:杨宇红,研究员,E-mail:yangyuhong@caas.cn
基金资助:
国家“十三·五”重点研发计划（2016YFD0201000）；国家公益性行业（农业）科研专项（201303014）；现代农业产业技术体系（CARS-25-B-01）；中国农业科学院创新工程（CAAS-ASTIP-IVFCAAS）；农业部园艺作物生物学与种质创制重点实验室项目

Synergistic Efficacy of BABA and Hrp-mutant of Ralstonia solanacearum against Pepper Blight

YANG Yuhong, MAO Zhenchuan, LING Jian, XIE Bingyan

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2017-04-07 Online:2017-08-08 Published:2017-08-11

摘要/Abstract

摘要： 将具有不同作用机理的植物化学诱抗剂β-氨基丁酸（BABA）与无致病力的茄青枯菌hrp-突变体菌株联合使用，研究不同配比对辣椒疫病的协同防治效果和使用技术。结果表明，BABA与hrp-菌株按一定配比混合后于接种前使用一次，与单剂相比对辣椒疫病均有不同程度的增效作用。其中0.08% BABA+hrp-菌株1×10⁷ cfu/mL、0.1% BABA+hrp-菌株1×10⁶ cfu/mL和0.1% BABA+hrp-菌株1×10⁵ cfu/mL等3种配比组合控制辣椒疫病的效果均在80%以上；与BABA单独使用相比，增效作用超过23%；以0.1% BABA+hrp-菌株1×10⁶ cfu/mL配比效果最佳，其增效达到43%。适当增加使用次数后，防治效果及持效期均得到进一步提高。本研究为探索多种生防措施的协调和配合应用开辟了一条新途径。

关键词: 辣椒疫霉, 生物防治, β-氨基丁酸, hrp-突变体, 协同增效

Abstract: The synergistic efficacy and application technique of the mixture of β-aminobutyric acid (BABA) and avirulent hrp-mutant of Ralstonia solanacearum against pepper blight with different proportion were determined. The results showed that pretreatment of the combined use of BABA and hrp-mutant before inoculation obviously increased the control effects on pepper blight compared with single agent. Among six combinations of BABA and hrp-mutant, pretreatment with 0.08% BABA+hrp-strain 1×10⁷cfu/mL, 0.1% BABA+hrp-strain 1×10⁶ cfu/mL, or 0.1% BABA+hrp-strain 1×10⁵ cfu/mL displayed the increase of control effect over 80%, and enhanced the synergy over 23%, compared with the single treatment with BABA. Moreover, pretreatment with the combined with 0.1% BABA+hrp-strain 1×10⁶ cfu/mL showed the best efficacy with the synergy of 43%. The control effect and duration of the efficacy against pepper blight were further improved by increasing the frequency of application treatment. This work explored a new way for revealing the coordination application of multiple biocontrol measures.

Key words: FPhytophthora capsici, biological control, β-aminobutyric acid, avirulent hrp-mutant, synergistic interaction

中图分类号:

S476

杨宇红, 茆振川, 凌键, 谢丙炎. β-氨基丁酸和茄青枯菌hrp-突变体协同防治辣椒疫病研究[J]. 中国生物防治学报, 2017, 33(4): 519-524.

YANG Yuhong, MAO Zhenchuan, LING Jian, XIE Bingyan. Synergistic Efficacy of BABA and Hrp-mutant of Ralstonia solanacearum against Pepper Blight[J]. journal1, 2017, 33(4): 519-524.

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β-氨基丁酸和茄青枯菌hrp-突变体协同防治辣椒疫病研究

Synergistic Efficacy of BABA and Hrp-mutant of Ralstonia solanacearum against Pepper Blight

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