变棕溶杆菌OH23次生代谢产物抗菌活性的分析及其发酵培养基优化

doi:10.16409/j.cnki.2095-039x.2019.03.009

中国生物防治学报 ›› 2019, Vol. 35 ›› Issue (3): 426-436.DOI: 10.16409/j.cnki.2095-039x.2019.03.009

变棕溶杆菌OH23次生代谢产物抗菌活性的分析及其发酵培养基优化

朱润杰^1,2, 赵延存², 凌军², 宋志伟², 赵杨扬², 薛雪¹, 刘凤权^1,2

1. 南京农业大学植物保护学院, 南京 210095;
2. 江苏省农业科学院植物保护研究所, 南京 210014

收稿日期:2018-10-10 出版日期:2019-06-08 发布日期:2019-06-17
通讯作者: 刘凤权,博士,研究员,E-mail:fqliu20011@sina.com
作者简介:朱润杰,硕士研究生,E-mail:zhurunjie1994@163.com
基金资助:
江苏省农业科技自主创新资金（CX-16-1049）；中国博士后面上项目（2017M610310）

Study on the Antibacterial Activity of Secondary Metabolites from Lysobacter brunescens OH23 and Optimization of Fermentation Medium

ZHU Runjie^1,2, ZHAO Yancun², LING Jun², SONG Zhiwei², ZHAO Yangyang², XUE Xue¹, LIU Fengquan^1,2

1. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;
2. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2018-10-10 Online:2019-06-08 Published:2019-06-17

摘要/Abstract

摘要： 本研究通过对变棕溶杆菌Lysobacter brunescens OH23菌株发酵产物的拮抗谱进行检测，发现其发酵产物对黄单胞属植物病原细菌具有特异拮抗活性，并且对水稻黄单胞菌不同菌株的拮抗活性存在差异。本研究进一步对OH23次生抗菌物质稳定性进行了分析，发现OH23产生的次生抗菌物质对高温、紫外光照、蛋白酶、非强碱性环境均表现稳定，但在强碱性环境（pH 11.0）下拮抗活性丧失。最后，采用单因素试验和正交试验设计对OH23发酵培养基组分进行了优化，最终确定OH23高产次生抗菌物质的培养基配方为高聚蛋白胨5 g/L、酵母粉5 g/L、可溶性淀粉6 g/L、FeSO₄·7H₂O 15 mg/L、ZnSO₄·7H₂O 5 mg/L。

关键词: 变棕溶杆菌, 水稻黄单胞菌, 次生代谢产物, 抗菌活性, 培养基优化

Abstract: In this study, the antimicrobial spectrum of secondary metabolites of Lysobacter brunescens OH23 was tested. The results showed that OH23 was a potential biocontrol strain with specific activity against Xanthomonas sp. At the same time, the secondary metabolites of OH23 showed different antagonistic activity against different strains of Xanthomonas oryzae. Stability test indicated that the active secondary metabolites were stable with high temperature, ultraviolet light, protease and non-strong alkaline environment. However, the antagonistic activity almost completely lost under strong alkaline conditions (pH=11.0). Single factor test and orthogonal design were used to screen and optimize the carbon source, nitrogen source and metal ion of OH23 fermentation medium. The final formulation for high yield of active secondary metabolites included high polypeptone at 5 g/L, yeast powder at 5 g/L, soluble starch at 6 g/L, FeSO₄·7H₂O at 15 mg/L and ZnSO₄·7H₂O at 5 mg/L.

Key words: Lysobacter brunescens, Xanthomonas oryzae, secondary metabolites, antibacterial activity, medium optimization

中图分类号:

S476

朱润杰, 赵延存, 凌军, 宋志伟, 赵杨扬, 薛雪, 刘凤权. 变棕溶杆菌OH23次生代谢产物抗菌活性的分析及其发酵培养基优化[J]. 中国生物防治学报, 2019, 35(3): 426-436.

ZHU Runjie, ZHAO Yancun, LING Jun, SONG Zhiwei, ZHAO Yangyang, XUE Xue, LIU Fengquan. Study on the Antibacterial Activity of Secondary Metabolites from Lysobacter brunescens OH23 and Optimization of Fermentation Medium[J]. journal1, 2019, 35(3): 426-436.

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变棕溶杆菌OH23次生代谢产物抗菌活性的分析及其发酵培养基优化

Study on the Antibacterial Activity of Secondary Metabolites from Lysobacter brunescens OH23 and Optimization of Fermentation Medium

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