小麦根部内生细菌ZY-1对小麦白粉菌的抑菌活性及拮抗机理

doi:10.16409/j.cnki.2095-039x.2016.05.013

中国生物防治学报 ›› 2016, Vol. 32 ›› Issue (5): 635-641.DOI: 10.16409/j.cnki.2095-039x.2016.05.013

小麦根部内生细菌ZY-1对小麦白粉菌的抑菌活性及拮抗机理

龚双军, 向礼波, 薛敏峰, 张学江, 史文琦, 曾凡松, 汪华, 杨立军, 喻大昭

湖北省农业科学院植物保护和土壤肥料研究所/农业部华中作物有害生物综合治理重点实验室/农作物重大病虫草害防控湖北省重点实验室, 武汉 430064

收稿日期:2016-03-16 出版日期:2016-10-08 发布日期:2016-10-08
通讯作者: 杨立军, 喻大昭
作者简介:龚双军,E-mail:gsj204@126.com
基金资助:
公益性行业（农业）科研专项（201303016）；小麦产业体系（CARS-03-04B）；湖北省农业科技创新中心项目（2014-620-003-003）

Antifungal Activity and Mechanism of an Endophytic Bacterium ZY-1 from Wheat Root against Blumerial graminis f. sp. Tritici

GONG Shuangjun, XIANG Libo, XUE Minfeng, ZHANG Xuejiang, SHI Wenqi, ZENG Fansong, WANG Hua, YANG Lijun, YU Dazhao

Key Laboratory of Integrated Pest Management on Crop in Central China, Ministry of Agriculture/Hubei Province Key Laboratory for Control of Crop Diseases, Pest and Weeds/Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, China

Received:2016-03-16 Online:2016-10-08 Published:2016-10-08

摘要/Abstract

摘要： 从小麦根部分离到6株代谢表面活性剂的菌株，比较各菌株排油性和抑菌性，结合PCR方法优选出一株具广谱抗菌性且含有srfA、fenA、ituB和bacD等4个关键基因的细菌ZY-1。经BIOLOG生理生化试验测定和16S rDNA序列系统发育学分析，将其鉴定为蜡样芽孢杆菌。平板和盆栽试验结果显示，菌株ZY-1的发酵液对活体专性寄生菌小麦白粉菌的生防效果分别达到（97.94±1.25）%和（82.56±2.11）%，优于10 mg/L的三唑酮化学药剂（P<0.05）。组织学显微观察表明，菌株ZY-1发酵液对小麦白粉病菌的分生孢子萌发和吸器形成具有极显著的抑制作用。产脂肽细菌ZY-1是一株应用前景广阔的生防菌。

关键词: 蜡样芽孢杆菌, 抗菌活性, 作用机制, 小麦白粉菌

Abstract: Six Bacillus strains isolated from wheat roots were evaluated for antifungal activity using oil-spreading and inhibitory tests. One strain, ZY-1, was found to have higher activity than the others, and PCR analysis revealed that it contained at least four antifungal genes including srfA, fenA, ituB, and bacD. Phylogenetic analysis of its 16S rDNA gene revealed that ZY-1 was a new strain of Bacillus cereus. Further investigation using in vitro plate assays and greenhouse experiments revealed that strain ZY-1 provided better control on Blumeria graminis f. sp. tritici than 10 mg/L applications of the chemical fungicide triadimefon, with control efficacies of 97.94%±1.25% and 82.56%±2.11%, respectively (P<0.05). In vivo histological analysis of the culture filtrate showed strain ZY-1 had a dramatic effect on the development of B. graminis, inhibiting both the germination of conidia and the formation of haustoria (P<0.05). Taken together, these results indicated that the lipopeptide-producing B. cereus stain ZY-1 has great potential as a biological control agent against B. graminis.

Key words: Bacillus cereus, antifungal activity, mechanism, Blumeria graminis f. sp. Tritici

中图分类号:

S476

龚双军, 向礼波, 薛敏峰, 张学江, 史文琦, 曾凡松, 汪华, 杨立军, 喻大昭. 小麦根部内生细菌ZY-1对小麦白粉菌的抑菌活性及拮抗机理[J]. 中国生物防治学报, 2016, 32(5): 635-641.

GONG Shuangjun, XIANG Libo, XUE Minfeng, ZHANG Xuejiang, SHI Wenqi, ZENG Fansong, WANG Hua, YANG Lijun, YU Dazhao. Antifungal Activity and Mechanism of an Endophytic Bacterium ZY-1 from Wheat Root against Blumerial graminis f. sp. Tritici[J]. journal1, 2016, 32(5): 635-641.

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小麦根部内生细菌ZY-1对小麦白粉菌的抑菌活性及拮抗机理

Antifungal Activity and Mechanism of an Endophytic Bacterium ZY-1 from Wheat Root against Blumerial graminis f. sp. Tritici

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