金龟子绿僵菌对石蒜绵粉蚧的室内毒力与防治效果

doi:10.16409/j.cnki.2095-039x.2019.06.009

中国生物防治学报 ›› 2019, Vol. 35 ›› Issue (6): 884-890.DOI: 10.16409/j.cnki.2095-039x.2019.06.009

金龟子绿僵菌对石蒜绵粉蚧的室内毒力与防治效果

黄鹏¹, 陈汉鑫², 姚锦爱¹, 黄建成¹, 余德亿¹

1. 福建省农业科学院植物保护研究所/福建省作物有害生物监测与治理重点实验室, 福州 350013;
2. 漳州市农业科学研究所, 漳州 363005

收稿日期:2019-06-12 出版日期:2019-12-08 发布日期:2019-12-18
通讯作者: 余德亿,研究员,E-mail:yudy_2004@126.com
作者简介:黄鹏,助理研究员,E-mail:375202928@qq.com。
基金资助:
福建省省属公益类科研院所基本科研专项（2018R1025-2，2016R1023-6）；福建省农业引导性（重点）项目（2017N0063）；福建省农业科学院项目（STTT2017-2-2，DEC201907，AC2017-12）

Laboratory Assessment of Pathogenicity of Metarhizium anisopliae against Phenacoccus solani

HUANG Peng¹, CHEN Hanxin², YAO Jinai¹, HUANG Jiancheng¹, YU Deyi¹

1. Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
2. Zhangzhou Institute of Agricultural Science, Zhangzhou 363005, China

Received:2019-06-12 Online:2019-12-08 Published:2019-12-18

摘要/Abstract

摘要： 石蒜绵粉蚧是近年我国新记录的一种有害生物，目前在福建省漳州地区的多肉植物种植基地发生为害严重。为探讨金龟子绿僵菌F061和FM-03对该虫的生防潜力和应用前景，本研究测定这2株菌株对石蒜绵粉蚧的室内毒力和防治效果。结果表明，石蒜绵粉蚧受菌株F061和FM-03侵染10 d后1×10⁸孢子/mL处理浓度的累计校正死亡率分别为83.15%和91.95%；构建的2个TDM模型均通过Pearson卡方和Hosmer-Lemeshow检验，2株菌株对石蒜绵粉蚧的时间-剂量互作效应明显，剂量效应随侵染时间的延长逐渐升高，时间效应随菌液浓度的升高逐渐增强，菌株F061侵染10 d后的LC₅₀和1.00×10⁸孢子/mL处理的LT₅₀分别为5.55×10⁵孢子/mL和5.04 d，而菌株FM-03在同等条件下的LC₅₀和LT₅₀分别为1.11×10⁵孢子/mL和4.67 d，毒力强于菌株F061；此外，2株菌株1.00×10⁸孢子/mL处理浓度对石蒜绵粉蚧的室内防治效果也随试验时间的延长而提高，菌剂F061和FM-03喷施10 d后的室内防治效果分别为74.06%和81.32%。综上，2株菌株均可用于防控多肉植物上的石蒜绵粉蚧，菌株FM-03可优先开发应用，菌株F061可作为备选菌株使用。

关键词: 金龟子绿僵菌, 石蒜绵粉蚧, 室内毒力, 室内防效

Abstract: Phenacoccus solani is a newly recorded pest in China. The pest has been posing a serious damage in succulent plants in Zhangzhou, Fujian province. In this study, we assayed the virulence and control efficiency of Metarhizium anisopliae F061 and FM-03 strains against P. solani in the laboratory, so as to evaluate the biocontrol potential and application prospect of the two entomopathogenic fungi strains against the insect pest. Results showed that the corrected accumulative mortality of P. solani were 83.15% and 91.95% when the pest was exposed to 1×10⁸ conidia/mL F061 and FM-03 in ten days, respectively. The Time-Dosage-Mortality (TDM) model showed a significant interaction of dosage-time effects of these two strains against P. solani, as indicated by the Pearson's chi-squared test and Hosmer-Lemeshow test. The dosage effect enhanced with the increase in infection time, or vice versa. The LC₅₀ of F061 was 5.55×10⁵ conidia/mL after an ten-day infection, and the LT₅₀ of 1.0×10⁸ conidia/mL was 5.04 d, both higher than those of FM-03 (1.11×10⁵ conidia/mL and 4.67 d), indicating higher virulence of FM-03 than F061. In addition, the laboratory contro1 efficiency of these two strains also increased with the increase in infection time, being 74.06% and 81.32% for 1×10⁸ conidia/mL of F061 and FM-03 in ten days, respectively. In conclusion, FM-03 can be preferentially used to control P. solani on succulent plants, while F061 can serve as an alternative strain.

Key words: Metarhizium anisopliae, Phenacoccus solani, laboratory virulence, laboratory contro1 efficiency

中图分类号:

S433
S476.1

黄鹏, 陈汉鑫, 姚锦爱, 黄建成, 余德亿. 金龟子绿僵菌对石蒜绵粉蚧的室内毒力与防治效果[J]. 中国生物防治学报, 2019, 35(6): 884-890.

HUANG Peng, CHEN Hanxin, YAO Jinai, HUANG Jiancheng, YU Deyi. Laboratory Assessment of Pathogenicity of Metarhizium anisopliae against Phenacoccus solani[J]. Chinese Journal Of Biological Control, 2019, 35(6): 884-890.

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金龟子绿僵菌对石蒜绵粉蚧的室内毒力与防治效果

Laboratory Assessment of Pathogenicity of Metarhizium anisopliae against Phenacoccus solani

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